Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway
Although the number of studies documenting the presence of Microplastics (MP) in fish is increasing, research studies focused on its detoxification are very limited. In this study, rainbow trout (Oncorhyncus mykiss) were randomly divided into two groups after being fed with MPs (15% polypropylene [P...
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| Format: | Article |
| Language: | English |
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Sciendo
2023-06-01
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| Series: | Oceanological and Hydrobiological Studies |
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| Online Access: | https://doi.org/10.26881/oahs-2023.2.05 |
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| author | Atamanalp Muhammed Kırıcı Muammer Köktürk Mine Kırıcı Mahinur Alwazeer Duried Kocaman Esat Mahmut Ucar Arzu Parlak Veysel Özcan Sinan Alak Gonca |
| author_facet | Atamanalp Muhammed Kırıcı Muammer Köktürk Mine Kırıcı Mahinur Alwazeer Duried Kocaman Esat Mahmut Ucar Arzu Parlak Veysel Özcan Sinan Alak Gonca |
| author_sort | Atamanalp Muhammed |
| collection | DOAJ |
| description | Although the number of studies documenting the presence of Microplastics (MP) in fish is increasing, research studies focused on its detoxification are very limited. In this study, rainbow trout (Oncorhyncus mykiss) were randomly divided into two groups after being fed with MPs (15% polypropylene [PP] +15% polyethylene [PE]) for 2 months. MP excretion without any application (PP+PE) in group I fish, and excretion of MPs with hydrogen-rich water (HRW) application (PP+PE+HRW) in group 2 were investigated under semi-static conditions for 21 days. This effect was also compared by using positive and negative control groups (Control [no treatment, free PP, PE or/and HRW] and only HRW group). In this direction, the following were determined: PP+PE chronic toxicity in aquatic organisms, the toxicity mechanism and the effect of HRW as a possible treatment method in blood tissue; with hematological indices ([RBC count [RBC], leukocyte count [WBC], hemoglobin value [Hb], hematocrit ratio [Hct], platelet count [PLT], hemoglobin count per erythrocyte [MCHC], mean hemoglobin amount per erythrocyte [MCH] and mean erythrocyte volume [MCV]) in other tissues (liver, gill and brain tissue) oxidative stress response (catalase [CAT]), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), glutathione (GSH), reactive oxygen products (ROS), malondialdehyde ([MDA] levels), DNA damage (8-OHdG: 8-Hydroxy-2-Deoxyguanosine), and the apoptosis (caspase 3) levels were investigated. In addition, acetylcholinesterase enzyme (AChE) activity, which is important in neurotoxicity pathways in the brain, was determined. The presence of plastics (PP/PE) in target tissues (muscle, liver, gill and gastrointestinal tract) was also obtained. |
| format | Article |
| id | doaj-art-e3ca83ced96348c1ae8a2e42ecaed830 |
| institution | Kabale University |
| issn | 1897-3191 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Oceanological and Hydrobiological Studies |
| spelling | doaj-art-e3ca83ced96348c1ae8a2e42ecaed8302025-01-20T11:10:23ZengSciendoOceanological and Hydrobiological Studies1897-31912023-06-0152220622010.26881/oahs-2023.2.05Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathwayAtamanalp Muhammed0Kırıcı Muammer1Köktürk Mine2Kırıcı Mahinur3Alwazeer Duried4Kocaman Esat Mahmut5Ucar Arzu6Parlak Veysel7Özcan Sinan8Alak Gonca9Department of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030Erzurum, TürkiyeDepartment of Veterinary Health, Food Agriculture and Livestock Vocational School, Bingöl University, Bingöl12000, TürkiyeDepartment of Organic Agriculture Management, Faculty of Applied Sciences, Igdir University, TR-76000Igdir, TürkiyeDepartment of Chemistry, Faculty of Arts and Science, Bingöl University, Bingöl12000, TürkiyeResearch Center for Redox Applications in Foods (RCRAF), Igdir University, 76000, Igdir, Turkey.Department of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030Erzurum, TürkiyeDepartment of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030Erzurum, TürkiyeDepartment of Basic Sciences, Faculty of Fisheries, Ataturk University, Erzurum25240, TürkiyeDepartment of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030Erzurum, TürkiyeDepartment of Seafood Processing Technology, Faculty of Fisheries, Ataturk University, Erzurum, TürkiyeAlthough the number of studies documenting the presence of Microplastics (MP) in fish is increasing, research studies focused on its detoxification are very limited. In this study, rainbow trout (Oncorhyncus mykiss) were randomly divided into two groups after being fed with MPs (15% polypropylene [PP] +15% polyethylene [PE]) for 2 months. MP excretion without any application (PP+PE) in group I fish, and excretion of MPs with hydrogen-rich water (HRW) application (PP+PE+HRW) in group 2 were investigated under semi-static conditions for 21 days. This effect was also compared by using positive and negative control groups (Control [no treatment, free PP, PE or/and HRW] and only HRW group). In this direction, the following were determined: PP+PE chronic toxicity in aquatic organisms, the toxicity mechanism and the effect of HRW as a possible treatment method in blood tissue; with hematological indices ([RBC count [RBC], leukocyte count [WBC], hemoglobin value [Hb], hematocrit ratio [Hct], platelet count [PLT], hemoglobin count per erythrocyte [MCHC], mean hemoglobin amount per erythrocyte [MCH] and mean erythrocyte volume [MCV]) in other tissues (liver, gill and brain tissue) oxidative stress response (catalase [CAT]), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), glutathione (GSH), reactive oxygen products (ROS), malondialdehyde ([MDA] levels), DNA damage (8-OHdG: 8-Hydroxy-2-Deoxyguanosine), and the apoptosis (caspase 3) levels were investigated. In addition, acetylcholinesterase enzyme (AChE) activity, which is important in neurotoxicity pathways in the brain, was determined. The presence of plastics (PP/PE) in target tissues (muscle, liver, gill and gastrointestinal tract) was also obtained.https://doi.org/10.26881/oahs-2023.2.05oxy-hydrogen gashydrogen-rich watermolecular hydrogenmps/npsblood indicesrainbow troutantioxidant statusfish |
| spellingShingle | Atamanalp Muhammed Kırıcı Muammer Köktürk Mine Kırıcı Mahinur Alwazeer Duried Kocaman Esat Mahmut Ucar Arzu Parlak Veysel Özcan Sinan Alak Gonca Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway Oceanological and Hydrobiological Studies oxy-hydrogen gas hydrogen-rich water molecular hydrogen mps/nps blood indices rainbow trout antioxidant status fish |
| title | Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway |
| title_full | Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway |
| title_fullStr | Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway |
| title_full_unstemmed | Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway |
| title_short | Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway |
| title_sort | does hydrogen rich water mitigate mp toxicity in rainbow trout oncorhyncus mykiss monitoring with hematology dna damage and apoptosis via ros gsh mda pathway |
| topic | oxy-hydrogen gas hydrogen-rich water molecular hydrogen mps/nps blood indices rainbow trout antioxidant status fish |
| url | https://doi.org/10.26881/oahs-2023.2.05 |
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