A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin
Protein purification is a critical step in both life sciences and biomanufacturing. Traditional affinity chromatography (AC) methods, including His-tag-based purification, provide high-purity proteins but are limited by the high cost of resins and the need for additional tag-removal steps. In this p...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2025-04-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5270&type=0 |
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| author | Xiaofeng Yang Zhanglin Lin Ya Xiang Binrui Chen Zisha Lao |
| author_facet | Xiaofeng Yang Zhanglin Lin Ya Xiang Binrui Chen Zisha Lao |
| author_sort | Xiaofeng Yang |
| collection | DOAJ |
| description | Protein purification is a critical step in both life sciences and biomanufacturing. Traditional affinity chromatography (AC) methods, including His-tag-based purification, provide high-purity proteins but are limited by the high cost of resins and the need for additional tag-removal steps. In this protocol, we present a reusable SpyDock-modified epoxy resin coupled with a pH-inducible self-cleaving intein for direct purification of proteins with authentic N-termini. This method enables efficient protein purification from cell lysates, achieving high purity (>90%) and yields comparable to the His-tag approach, without requiring tag removal. The SpyDock-modified resin protocol is robust, easy to implement, and cost-effective, making it suitable for both research and large-scale industrial applications. |
| format | Article |
| id | doaj-art-e3b4e9ccd3ee41ac8def98cb768ad62e |
| institution | DOAJ |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-e3b4e9ccd3ee41ac8def98cb768ad62e2025-08-20T03:11:21ZengBio-protocol LLCBio-Protocol2331-83252025-04-0115810.21769/BioProtoc.5270A Robust and Easy Protein Purification Method Using SpyDock-Modified ResinXiaofeng Yang0Zhanglin Lin1Ya Xiang2Binrui Chen3Zisha Lao4School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, ChinaProtein purification is a critical step in both life sciences and biomanufacturing. Traditional affinity chromatography (AC) methods, including His-tag-based purification, provide high-purity proteins but are limited by the high cost of resins and the need for additional tag-removal steps. In this protocol, we present a reusable SpyDock-modified epoxy resin coupled with a pH-inducible self-cleaving intein for direct purification of proteins with authentic N-termini. This method enables efficient protein purification from cell lysates, achieving high purity (>90%) and yields comparable to the His-tag approach, without requiring tag removal. The SpyDock-modified resin protocol is robust, easy to implement, and cost-effective, making it suitable for both research and large-scale industrial applications.https://bio-protocol.org/en/bpdetail?id=5270&type=0 |
| spellingShingle | Xiaofeng Yang Zhanglin Lin Ya Xiang Binrui Chen Zisha Lao A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin Bio-Protocol |
| title | A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin |
| title_full | A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin |
| title_fullStr | A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin |
| title_full_unstemmed | A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin |
| title_short | A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin |
| title_sort | robust and easy protein purification method using spydock modified resin |
| url | https://bio-protocol.org/en/bpdetail?id=5270&type=0 |
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