Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis
Background: The mortality rate of meningitis is still alarmingly high in certain regions across the globe. The objective of this research is to identify the most effective primers for detecting Streptococcus (S.) pneumoniae, Haemophilus (H.) influenzae, and Neisseria (N.) meningitidis using Real-Tim...
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| Format: | Article |
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German Medical Science GMS Publishing House
2025-04-01
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| Series: | GMS Hygiene and Infection Control |
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| Online Access: | https://journals.publisso.de/en/journals/hic/volume20/dgkh000545 |
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| author | Azimi, Leila Shirkavand, Fatemeh Armin, Shahnaz Karbasian, Fereshteh Khodaei, Hannan |
| author_facet | Azimi, Leila Shirkavand, Fatemeh Armin, Shahnaz Karbasian, Fereshteh Khodaei, Hannan |
| author_sort | Azimi, Leila |
| collection | DOAJ |
| description | Background: The mortality rate of meningitis is still alarmingly high in certain regions across the globe. The objective of this research is to identify the most effective primers for detecting Streptococcus (S.) pneumoniae, Haemophilus (H.) influenzae, and Neisseria (N.) meningitidis using Real-Time PCR technology.
Materials and methods: Two sets of primers were developed for detecting S. pneumoniae, H. influenzae, and N. meningitidis using the Primer Biosoft Allele ID 7.6 application. The study examined the minimum bacterial copy numbers detectable by each primer, as well as their specificity.
Results: CtrA and hpd2 could detect the 400 copy numbers/ml of H. influenzae, and N. meningitidis and LytA2 could detect the 40 copy numbers/ml of S. pneumoniae. The sensitivity and specificity of all primers was 100% (CI: 95%).
Conclusion: Using more sensitive primers to detect the bacterial agent responsible for causing bacterial meningitis increases the chance of identifying the causative bacteria. The primers designed in this study could identify the selected bacteria with at least 10 times more sensitivity than the currently available commercial diagnostic kits in Iran. |
| format | Article |
| id | doaj-art-e3afecc9c2124372bbb5c9c751acd821 |
| institution | OA Journals |
| issn | 2196-5226 |
| language | deu |
| publishDate | 2025-04-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj-art-e3afecc9c2124372bbb5c9c751acd8212025-08-20T02:32:38ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262025-04-0120Doc1610.3205/dgkh000545Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidisAzimi, Leila0https://orcid.org/0000-0002-7216-2530Shirkavand, Fatemeh1https://orcid.org/0009-0001-5207-4883Armin, Shahnaz2https://orcid.org/0000-0002-4993-482XKarbasian, Fereshteh3https://orcid.org/0000-0003-2494-6231Khodaei, Hannan4https://orcid.org/0000-0002-2297-4733Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, IranPediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, IranPediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of pediatric gastroenterology and hepatology, Ali-Asghar children’s hospital, Iran University of Medical Sciences, Tehran, IranPediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, IranBackground: The mortality rate of meningitis is still alarmingly high in certain regions across the globe. The objective of this research is to identify the most effective primers for detecting Streptococcus (S.) pneumoniae, Haemophilus (H.) influenzae, and Neisseria (N.) meningitidis using Real-Time PCR technology. Materials and methods: Two sets of primers were developed for detecting S. pneumoniae, H. influenzae, and N. meningitidis using the Primer Biosoft Allele ID 7.6 application. The study examined the minimum bacterial copy numbers detectable by each primer, as well as their specificity. Results: CtrA and hpd2 could detect the 400 copy numbers/ml of H. influenzae, and N. meningitidis and LytA2 could detect the 40 copy numbers/ml of S. pneumoniae. The sensitivity and specificity of all primers was 100% (CI: 95%). Conclusion: Using more sensitive primers to detect the bacterial agent responsible for causing bacterial meningitis increases the chance of identifying the causative bacteria. The primers designed in this study could identify the selected bacteria with at least 10 times more sensitivity than the currently available commercial diagnostic kits in Iran.https://journals.publisso.de/en/journals/hic/volume20/dgkh000545streptococcus pneumoniaehaemophilus influenzaeneisseria meningitidisprimersensitivity |
| spellingShingle | Azimi, Leila Shirkavand, Fatemeh Armin, Shahnaz Karbasian, Fereshteh Khodaei, Hannan Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis GMS Hygiene and Infection Control streptococcus pneumoniae haemophilus influenzae neisseria meningitidis primer sensitivity |
| title | Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis |
| title_full | Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis |
| title_fullStr | Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis |
| title_full_unstemmed | Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis |
| title_short | Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis |
| title_sort | design and development of primers for detection of streptococcus pneumoniae haemophilus influenzae and neisseria meningitidis |
| topic | streptococcus pneumoniae haemophilus influenzae neisseria meningitidis primer sensitivity |
| url | https://journals.publisso.de/en/journals/hic/volume20/dgkh000545 |
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