Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology

The aim of this study was to develop a novel kefir beverage using date syrup, whey permeate, and whey. The levels of the kefir grain inoculum (2–5% w/v), fruit syrup (10–50% w/v), and whey permeate (0–5% w/v) on pH, total phenolic content, antioxidant activity, lactic acid bacteria and yeast counts,...

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Main Authors: Sana M’hir, Kais Rtibi, Asma Mejri, Manel Ziadi, Hajer Aloui, Moktar Hamdi, Lamia Ayed
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2019/1218058
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author Sana M’hir
Kais Rtibi
Asma Mejri
Manel Ziadi
Hajer Aloui
Moktar Hamdi
Lamia Ayed
author_facet Sana M’hir
Kais Rtibi
Asma Mejri
Manel Ziadi
Hajer Aloui
Moktar Hamdi
Lamia Ayed
author_sort Sana M’hir
collection DOAJ
description The aim of this study was to develop a novel kefir beverage using date syrup, whey permeate, and whey. The levels of the kefir grain inoculum (2–5% w/v), fruit syrup (10–50% w/v), and whey permeate (0–5% w/v) on pH, total phenolic content, antioxidant activity, lactic acid bacteria and yeast counts, and overall acceptability were investigated using central composite design. The use of response surface methodology allowed us to obtain a formulation with acceptable organoleptic properties and high antioxidant activities. The obtained beverages had total phenolic content, % DPPH scavenging activity, and overall acceptability ranging from 24 to 74 mg GAE/mL, from 74.80 to 91.37 mg GAE/mL, and from 3.50 to 6 mg GAE/mL (based on a 1 to 9 preference scale), respectively. Date syrup of 36.76% (w/v), whey permeates of 2.99%, and kefir grains inoculum size of 2.08% were the optimized process conditions achieved.
format Article
id doaj-art-e394fbecfb464a689e42e9e97d025759
institution Kabale University
issn 2090-9063
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language English
publishDate 2019-01-01
publisher Wiley
record_format Article
series Journal of Chemistry
spelling doaj-art-e394fbecfb464a689e42e9e97d0257592025-02-03T05:50:41ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/12180581218058Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface MethodologySana M’hir0Kais Rtibi1Asma Mejri2Manel Ziadi3Hajer Aloui4Moktar Hamdi5Lamia Ayed6Laboratory of Microbial Ecology and Technology (LETMI), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, BP 676, 1080 Tunis, TunisiaLaboratory of Functional Physiology and Valorization of Bio-resources, Higher Institute of Biotechnology of Beja, University of Jendouba, BP 382, 9000 Beja, TunisiaLaboratory of Microbial Ecology and Technology (LETMI), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, BP 676, 1080 Tunis, TunisiaLaboratory of Microbial Ecology and Technology (LETMI), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, BP 676, 1080 Tunis, TunisiaInstitut National de Recherche et d’Analyse Physico-Chimique, Laboratoire des Substances Naturelles, Tunis, TunisiaLaboratory of Microbial Ecology and Technology (LETMI), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, BP 676, 1080 Tunis, TunisiaLaboratory of Microbial Ecology and Technology (LETMI), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, BP 676, 1080 Tunis, TunisiaThe aim of this study was to develop a novel kefir beverage using date syrup, whey permeate, and whey. The levels of the kefir grain inoculum (2–5% w/v), fruit syrup (10–50% w/v), and whey permeate (0–5% w/v) on pH, total phenolic content, antioxidant activity, lactic acid bacteria and yeast counts, and overall acceptability were investigated using central composite design. The use of response surface methodology allowed us to obtain a formulation with acceptable organoleptic properties and high antioxidant activities. The obtained beverages had total phenolic content, % DPPH scavenging activity, and overall acceptability ranging from 24 to 74 mg GAE/mL, from 74.80 to 91.37 mg GAE/mL, and from 3.50 to 6 mg GAE/mL (based on a 1 to 9 preference scale), respectively. Date syrup of 36.76% (w/v), whey permeates of 2.99%, and kefir grains inoculum size of 2.08% were the optimized process conditions achieved.http://dx.doi.org/10.1155/2019/1218058
spellingShingle Sana M’hir
Kais Rtibi
Asma Mejri
Manel Ziadi
Hajer Aloui
Moktar Hamdi
Lamia Ayed
Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology
Journal of Chemistry
title Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology
title_full Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology
title_fullStr Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology
title_full_unstemmed Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology
title_short Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology
title_sort development of a novel whey date beverage fermented with kefir grains using response surface methodology
url http://dx.doi.org/10.1155/2019/1218058
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