Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus
Considering that counting the percentage of CD4 T lymphocytes can add prognostic information regarding patients infected with HIV, the aim of this study was to evaluate the percentage values of CD4+ T lymphocytes from 81 patients determined by flow cytometry and estimated by flow cytometry in conjun...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/906873 |
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| author | Danielle Cristyane Kalva Borato Emerson Carraro Sônia Regina Weber Ribas Carlos Augusto Kalva-Filho José Carlos Rebuglio Vellosa |
| author_facet | Danielle Cristyane Kalva Borato Emerson Carraro Sônia Regina Weber Ribas Carlos Augusto Kalva-Filho José Carlos Rebuglio Vellosa |
| author_sort | Danielle Cristyane Kalva Borato |
| collection | DOAJ |
| description | Considering that counting the percentage of CD4 T lymphocytes can add prognostic information regarding patients infected with HIV, the aim of this study was to evaluate the percentage values of CD4+ T lymphocytes from 81 patients determined by flow cytometry and estimated by flow cytometry in conjunction with a hematology counter. Means were compared through the Student's t-test. Pearson's correlation was determined, and the agreement between results was tested by Bland-Altman. The level of significance was P<0.05. It was found a significantly higher mean difference between the relative values of CD4+ T lymphocytes to the hematologic counter (P<0.05), for all strata studied. Positive and significant correlations (P<0.01) were found between the strata CD4 < 200 cells/mL (r=0.93), between 200 and 500 cells/mL (r=0.65), and >500 cells/mL (r=0.81). The limits of agreement were 1.0±3.8% for the stratum of CD4 < 200 cells/mL, approximately 2.2±13.5% for the stratum of CD4 between 200 and 500 cells/mL, and approximately 6.2±20.4% for the stratum > 500 cells/mL. The differences in the percentages of CD4+ T lymphocytes obtained by different methodologies could lead to conflict when used in clinical decisions related to the treatment and care of people infected with HIV. |
| format | Article |
| id | doaj-art-e385410d97d245238de585a54105f3e9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e385410d97d245238de585a54105f3e92025-02-03T05:51:50ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/906873906873Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency VirusDanielle Cristyane Kalva Borato0Emerson Carraro1Sônia Regina Weber Ribas2Carlos Augusto Kalva-Filho3José Carlos Rebuglio Vellosa4Pharmaceutical Sciences Post Graduate Program, State University of Ponta Grossa (UEPG), General Carlos Cavalcanti Avenue, 4748 Uvaranas, 84030-900 Ponta Grossa, PR, BrazilDepartment of Pharmacy, State University in the Paraná Midwestern (UNICENTRO), CEDETEG Campus, 85040-080 Guarapuava, PR, BrazilCounty Health Department, Expert Assistance Service (EAS), City Hall, 84051-900 Ponta Grossa, PR, BrazilDepartment of Physiotherapy, State University Paulista (UNESP), Presidente Prudente Campus, 19060-900 Presidente Prudente, SP, BrazilPharmaceutical Sciences Post Graduate Program, State University of Ponta Grossa (UEPG), General Carlos Cavalcanti Avenue, 4748 Uvaranas, 84030-900 Ponta Grossa, PR, BrazilConsidering that counting the percentage of CD4 T lymphocytes can add prognostic information regarding patients infected with HIV, the aim of this study was to evaluate the percentage values of CD4+ T lymphocytes from 81 patients determined by flow cytometry and estimated by flow cytometry in conjunction with a hematology counter. Means were compared through the Student's t-test. Pearson's correlation was determined, and the agreement between results was tested by Bland-Altman. The level of significance was P<0.05. It was found a significantly higher mean difference between the relative values of CD4+ T lymphocytes to the hematologic counter (P<0.05), for all strata studied. Positive and significant correlations (P<0.01) were found between the strata CD4 < 200 cells/mL (r=0.93), between 200 and 500 cells/mL (r=0.65), and >500 cells/mL (r=0.81). The limits of agreement were 1.0±3.8% for the stratum of CD4 < 200 cells/mL, approximately 2.2±13.5% for the stratum of CD4 between 200 and 500 cells/mL, and approximately 6.2±20.4% for the stratum > 500 cells/mL. The differences in the percentages of CD4+ T lymphocytes obtained by different methodologies could lead to conflict when used in clinical decisions related to the treatment and care of people infected with HIV.http://dx.doi.org/10.1100/2012/906873 |
| spellingShingle | Danielle Cristyane Kalva Borato Emerson Carraro Sônia Regina Weber Ribas Carlos Augusto Kalva-Filho José Carlos Rebuglio Vellosa Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus The Scientific World Journal |
| title | Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
| title_full | Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
| title_fullStr | Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
| title_full_unstemmed | Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
| title_short | Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
| title_sort | comparison of two methodologies for cd4 t lymphocytes relative counting on immune monitoring of patients with human immunodeficiency virus |
| url | http://dx.doi.org/10.1100/2012/906873 |
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