Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed
The Zabaikalskaya horse is an indigenous breed of horses from Siberia with diverse use. It is characterized by e durance and good adaptability to year-round herd maintenance in the harsh conditions of the Baikal steppes. To determine the genetic characteristics of the maternal lineage of the Zabaika...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2021-09-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/3106 |
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| author | L. A. Khrabrova N. V. Blohina B. Z. Bazaron T. N. Khamiruev |
| author_facet | L. A. Khrabrova N. V. Blohina B. Z. Bazaron T. N. Khamiruev |
| author_sort | L. A. Khrabrova |
| collection | DOAJ |
| description | The Zabaikalskaya horse is an indigenous breed of horses from Siberia with diverse use. It is characterized by e durance and good adaptability to year-round herd maintenance in the harsh conditions of the Baikal steppes. To determine the genetic characteristics of the maternal lineage of the Zabaikalskaya horse breed based on mitochondrial DNA polymorphisms, we collected hair samples from 31 horses belonging to breeding farms in the Trans-Baikal Territory. Analysis of the 530 bp sequence of the mtDNA D-loop was performed using the maximum composite likelihood (MCL) model in combination with bootstrap analysis. When studying the polymorphism of the hypervariable region of the mtDNA D-loop in Zabaikalskaya horses, we identified 31 haplotypes representing 8 haplogroups: B, C, G, H, L, M, Q and R according to modern classification. The sequenced fragment of the D-loop from nucleotide position 15471 to 16000 contained 17 polymorphic sites, mainly represented by the A→G, G→A and T→C transitions. The haplogroups Q (25.81 %), B (19.35 %), G (16.13 %) and H (12.90 %) were prevailing in the mtDNA structure of this breed. Genetic analysis of the mitochondrial genome of the Zabaikalskaya horse revealed a high level of diversity of haplotypes and haplogroups, which are typical for the horse populations of Eurasia. |
| format | Article |
| id | doaj-art-e3636fb0796d48c985d983b2d378da28 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-e3636fb0796d48c985d983b2d378da282025-02-01T09:58:10ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592021-09-0125548649110.18699/VJ21.0551182Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breedL. A. Khrabrova0N. V. Blohina1B. Z. Bazaron2T. N. Khamiruev3All-Russian Research Institute for Horse BreedingAll-Russian Research Institute for Horse BreedingScientific Research Institute of Veterinary Medicine of Eastern Siberia – Branch of the Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of SciencesScientific Research Institute of Veterinary Medicine of Eastern Siberia – Branch of the Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of SciencesThe Zabaikalskaya horse is an indigenous breed of horses from Siberia with diverse use. It is characterized by e durance and good adaptability to year-round herd maintenance in the harsh conditions of the Baikal steppes. To determine the genetic characteristics of the maternal lineage of the Zabaikalskaya horse breed based on mitochondrial DNA polymorphisms, we collected hair samples from 31 horses belonging to breeding farms in the Trans-Baikal Territory. Analysis of the 530 bp sequence of the mtDNA D-loop was performed using the maximum composite likelihood (MCL) model in combination with bootstrap analysis. When studying the polymorphism of the hypervariable region of the mtDNA D-loop in Zabaikalskaya horses, we identified 31 haplotypes representing 8 haplogroups: B, C, G, H, L, M, Q and R according to modern classification. The sequenced fragment of the D-loop from nucleotide position 15471 to 16000 contained 17 polymorphic sites, mainly represented by the A→G, G→A and T→C transitions. The haplogroups Q (25.81 %), B (19.35 %), G (16.13 %) and H (12.90 %) were prevailing in the mtDNA structure of this breed. Genetic analysis of the mitochondrial genome of the Zabaikalskaya horse revealed a high level of diversity of haplotypes and haplogroups, which are typical for the horse populations of Eurasia.https://vavilov.elpub.ru/jour/article/view/3106genetic diversityhaplogroups mtdnahorsephylogenetic analysiszabaikalskaya breed |
| spellingShingle | L. A. Khrabrova N. V. Blohina B. Z. Bazaron T. N. Khamiruev Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed Вавиловский журнал генетики и селекции genetic diversity haplogroups mtdna horse phylogenetic analysis zabaikalskaya breed |
| title | Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed |
| title_full | Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed |
| title_fullStr | Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed |
| title_full_unstemmed | Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed |
| title_short | Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed |
| title_sort | variability of mitochondrial dna d loop sequences in zabaikalskaya horse breed |
| topic | genetic diversity haplogroups mtdna horse phylogenetic analysis zabaikalskaya breed |
| url | https://vavilov.elpub.ru/jour/article/view/3106 |
| work_keys_str_mv | AT lakhrabrova variabilityofmitochondrialdnadloopsequencesinzabaikalskayahorsebreed AT nvblohina variabilityofmitochondrialdnadloopsequencesinzabaikalskayahorsebreed AT bzbazaron variabilityofmitochondrialdnadloopsequencesinzabaikalskayahorsebreed AT tnkhamiruev variabilityofmitochondrialdnadloopsequencesinzabaikalskayahorsebreed |