Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus
The distribution of Culex quinquefasciatus as a vector for several tropical diseases in Indonesia could affect the transmission of various pathogens, including filariasis, West Nile, encephalitis, and Rift Valley fever. The use of insecticides to control mosquito populations is carried out massivel...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2025-07-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/46670 |
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| author | Dzikra Nasyaya Mahfudhah Fistara Lesti Rahmafitria Teguh Hari Sucipto Shifa Fauziyah Mirwa Adiprahara Anggarani Nuniek Herdyastuti Sin War Naw Adiana Mutamsari Witaningrum |
| author_facet | Dzikra Nasyaya Mahfudhah Fistara Lesti Rahmafitria Teguh Hari Sucipto Shifa Fauziyah Mirwa Adiprahara Anggarani Nuniek Herdyastuti Sin War Naw Adiana Mutamsari Witaningrum |
| author_sort | Dzikra Nasyaya Mahfudhah |
| collection | DOAJ |
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The distribution of Culex quinquefasciatus as a vector for several tropical diseases in Indonesia could affect the transmission of various pathogens, including filariasis, West Nile, encephalitis, and Rift Valley fever. The use of insecticides to control mosquito populations is carried out massively. However, this effort is threatened by an increase in mosquito resistance to insecticides, in this case, pyrethroids that target Voltage-gated sodium channel (VGSC) as a complex protein in mosquito nerve cells that plays a role in the movement of sodium ions, where these compounds can have a knockdown effect and lead to death. The existence of a single nucleotide mutation in this specific codon results in knockdown resistance (kdr) in mosquitoes. This study aims to determine whether there is a kdr mutation in the Vgsc-L1014F target using the latest molecular method ETAS-PCR to type tri-allelic variation at Vgsc-1014 in Cx. quinquefasciatus samples collected in three areas in the cities of Surabaya and Sidoarjo, namely Wonocolo, Sukodono, and Wonoayu. The result showed ten male pool samples (C1-C10) of Cx. quinquefasciatus did not carry mutations or present as homozygous wild type (TTT/TTT), with a DNA band size of 181 bp. Hence, this preliminary study could be extended for further research to build vector control monitoring programs.
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| format | Article |
| id | doaj-art-e34fee8d69e2440997f84331a2cb658d |
| institution | DOAJ |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-e34fee8d69e2440997f84331a2cb658d2025-08-20T02:39:48ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942025-07-0132610.4308/hjb.32.6.1400-1406Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatusDzikra Nasyaya Mahfudhah0Fistara Lesti Rahmafitria1Teguh Hari Sucipto2Shifa Fauziyah3Mirwa Adiprahara Anggarani4Nuniek Herdyastuti5Sin War Naw6Adiana Mutamsari Witaningrum7Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya 60231, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya 60231, IndonesiaDengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, IndonesiaDelima Husada Gresik Health Academy, Gresik 61111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya 60231, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya 60231, IndonesiaDepartment of Chemistry, Myitkyina University, MyanmarDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia The distribution of Culex quinquefasciatus as a vector for several tropical diseases in Indonesia could affect the transmission of various pathogens, including filariasis, West Nile, encephalitis, and Rift Valley fever. The use of insecticides to control mosquito populations is carried out massively. However, this effort is threatened by an increase in mosquito resistance to insecticides, in this case, pyrethroids that target Voltage-gated sodium channel (VGSC) as a complex protein in mosquito nerve cells that plays a role in the movement of sodium ions, where these compounds can have a knockdown effect and lead to death. The existence of a single nucleotide mutation in this specific codon results in knockdown resistance (kdr) in mosquitoes. This study aims to determine whether there is a kdr mutation in the Vgsc-L1014F target using the latest molecular method ETAS-PCR to type tri-allelic variation at Vgsc-1014 in Cx. quinquefasciatus samples collected in three areas in the cities of Surabaya and Sidoarjo, namely Wonocolo, Sukodono, and Wonoayu. The result showed ten male pool samples (C1-C10) of Cx. quinquefasciatus did not carry mutations or present as homozygous wild type (TTT/TTT), with a DNA band size of 181 bp. Hence, this preliminary study could be extended for further research to build vector control monitoring programs. https://journal.ipb.ac.id/index.php/hayati/article/view/46670 |
| spellingShingle | Dzikra Nasyaya Mahfudhah Fistara Lesti Rahmafitria Teguh Hari Sucipto Shifa Fauziyah Mirwa Adiprahara Anggarani Nuniek Herdyastuti Sin War Naw Adiana Mutamsari Witaningrum Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus Hayati Journal of Biosciences |
| title | Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus |
| title_full | Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus |
| title_fullStr | Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus |
| title_full_unstemmed | Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus |
| title_short | Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus |
| title_sort | detection of vgsc l1014f allele knockdown resistance mutation in male culex quinquefasciatus |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/46670 |
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