Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants.
RNA silencing is one of the main defense mechanisms employed by plants to fight viruses. In change, viruses have evolved silencing suppressor proteins to neutralize antiviral silencing. Since the endogenous and antiviral functions of RNA silencing pathway rely on common components, it was suggested...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-10-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005935&type=printable |
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| author | Levente Kontra Tibor Csorba Mario Tavazza Alessandra Lucioli Raffaela Tavazza Simon Moxon Viktória Tisza Anna Medzihradszky Massimo Turina József Burgyán |
| author_facet | Levente Kontra Tibor Csorba Mario Tavazza Alessandra Lucioli Raffaela Tavazza Simon Moxon Viktória Tisza Anna Medzihradszky Massimo Turina József Burgyán |
| author_sort | Levente Kontra |
| collection | DOAJ |
| description | RNA silencing is one of the main defense mechanisms employed by plants to fight viruses. In change, viruses have evolved silencing suppressor proteins to neutralize antiviral silencing. Since the endogenous and antiviral functions of RNA silencing pathway rely on common components, it was suggested that viral suppressors interfere with endogenous silencing pathway contributing to viral symptom development. In this work, we aimed to understand the effects of the tombusviral p19 suppressor on endogenous and antiviral silencing during genuine virus infection. We showed that ectopically expressed p19 sequesters endogenous small RNAs (sRNAs) in the absence, but not in the presence of virus infection. Our presented data question the generalized model in which the sequestration of endogenous sRNAs by the viral suppressor contributes to the viral symptom development. We further showed that p19 preferentially binds the perfectly paired ds-viral small interfering RNAs (vsiRNAs) but does not select based on their sequence or the type of the 5' nucleotide. Finally, co-immunoprecipitation of sRNAs with AGO1 or AGO2 from virus-infected plants revealed that p19 specifically impairs vsiRNA loading into AGO1 but not AGO2. Our findings, coupled with the fact that p19-expressing wild type Cymbidium ringspot virus (CymRSV) overcomes the Nicotiana benthamiana silencing based defense killing the host, suggest that AGO1 is the main effector of antiviral silencing in this host-virus combination. |
| format | Article |
| id | doaj-art-e31a580c034f4e2ebd46ffdd4e672272 |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2016-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-e31a580c034f4e2ebd46ffdd4e6722722025-08-20T02:45:37ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-10-011210e100593510.1371/journal.ppat.1005935Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants.Levente KontraTibor CsorbaMario TavazzaAlessandra LucioliRaffaela TavazzaSimon MoxonViktória TiszaAnna MedzihradszkyMassimo TurinaJózsef BurgyánRNA silencing is one of the main defense mechanisms employed by plants to fight viruses. In change, viruses have evolved silencing suppressor proteins to neutralize antiviral silencing. Since the endogenous and antiviral functions of RNA silencing pathway rely on common components, it was suggested that viral suppressors interfere with endogenous silencing pathway contributing to viral symptom development. In this work, we aimed to understand the effects of the tombusviral p19 suppressor on endogenous and antiviral silencing during genuine virus infection. We showed that ectopically expressed p19 sequesters endogenous small RNAs (sRNAs) in the absence, but not in the presence of virus infection. Our presented data question the generalized model in which the sequestration of endogenous sRNAs by the viral suppressor contributes to the viral symptom development. We further showed that p19 preferentially binds the perfectly paired ds-viral small interfering RNAs (vsiRNAs) but does not select based on their sequence or the type of the 5' nucleotide. Finally, co-immunoprecipitation of sRNAs with AGO1 or AGO2 from virus-infected plants revealed that p19 specifically impairs vsiRNA loading into AGO1 but not AGO2. Our findings, coupled with the fact that p19-expressing wild type Cymbidium ringspot virus (CymRSV) overcomes the Nicotiana benthamiana silencing based defense killing the host, suggest that AGO1 is the main effector of antiviral silencing in this host-virus combination.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005935&type=printable |
| spellingShingle | Levente Kontra Tibor Csorba Mario Tavazza Alessandra Lucioli Raffaela Tavazza Simon Moxon Viktória Tisza Anna Medzihradszky Massimo Turina József Burgyán Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants. PLoS Pathogens |
| title | Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants. |
| title_full | Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants. |
| title_fullStr | Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants. |
| title_full_unstemmed | Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants. |
| title_short | Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants. |
| title_sort | distinct effects of p19 rna silencing suppressor on small rna mediated pathways in plants |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005935&type=printable |
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