Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions
Background. In many European countries, patients with a variety of chronical inflammatory diseases are treated with low-dose radiotherapy (LD-RT). In contrast to high-dose irradiation given to tumor patients, little is known about radiobiological mechanisms underlying this clinical successful LD-RT...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/2856518 |
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| author | Sabine Schröder Stephan Kriesen Daniel Paape Guido Hildebrandt Katrin Manda |
| author_facet | Sabine Schröder Stephan Kriesen Daniel Paape Guido Hildebrandt Katrin Manda |
| author_sort | Sabine Schröder |
| collection | DOAJ |
| description | Background. In many European countries, patients with a variety of chronical inflammatory diseases are treated with low-dose radiotherapy (LD-RT). In contrast to high-dose irradiation given to tumor patients, little is known about radiobiological mechanisms underlying this clinical successful LD-RT application. The objective of this study was to gain a better insight into the modulation of inflammatory reactions after LD-RT on the basis of endothelial cells (EC) as major participants and regulators of inflammation. Methods. Three murine EC lines were cultivated under 2D and 3D culture conditions and irradiated with doses from 0.01 Gy to 2 Gy. To simulate an inflammatory situation, cells were activated with TNF-α. After LD-RT, a screening of numerous inflammatory markers was determined by multiplex assay, followed by detailed analyses of four cytokines (KC, MCP-1, RANTES, and G-CSF). Additionally, the monocyte binding to EC was analyzed. Results. Cytokine concentrations were dependent on culture condition, IR dose, time point after IR, and EC origin. IR caused nonlinear dose-dependent effects on secretion of the proinflammatory cytokines KC, MCP-1, and RANTES. The monocyte adhesion was significantly enhanced after IR as well as activation. Conclusions. The study shows that LD-RT, also using very low radiation doses, has a clear immunomodulatory effect on EC as major participants and regulators of inflammation. |
| format | Article |
| id | doaj-art-e319db85c6aa4a6882ced64817ed7458 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e319db85c6aa4a6882ced64817ed74582025-02-03T05:51:04ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/28565182856518Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture ConditionsSabine Schröder0Stephan Kriesen1Daniel Paape2Guido Hildebrandt3Katrin Manda4Department of Radiotherapy and Radiation Oncology, University Medical Center Rostock, Südring 75, 18059 Rostock, GermanyDepartment of Radiotherapy and Radiation Oncology, University Medical Center Rostock, Südring 75, 18059 Rostock, GermanyDepartment of Radiotherapy and Radiation Oncology, University Medical Center Rostock, Südring 75, 18059 Rostock, GermanyDepartment of Radiotherapy and Radiation Oncology, University Medical Center Rostock, Südring 75, 18059 Rostock, GermanyDepartment of Radiotherapy and Radiation Oncology, University Medical Center Rostock, Südring 75, 18059 Rostock, GermanyBackground. In many European countries, patients with a variety of chronical inflammatory diseases are treated with low-dose radiotherapy (LD-RT). In contrast to high-dose irradiation given to tumor patients, little is known about radiobiological mechanisms underlying this clinical successful LD-RT application. The objective of this study was to gain a better insight into the modulation of inflammatory reactions after LD-RT on the basis of endothelial cells (EC) as major participants and regulators of inflammation. Methods. Three murine EC lines were cultivated under 2D and 3D culture conditions and irradiated with doses from 0.01 Gy to 2 Gy. To simulate an inflammatory situation, cells were activated with TNF-α. After LD-RT, a screening of numerous inflammatory markers was determined by multiplex assay, followed by detailed analyses of four cytokines (KC, MCP-1, RANTES, and G-CSF). Additionally, the monocyte binding to EC was analyzed. Results. Cytokine concentrations were dependent on culture condition, IR dose, time point after IR, and EC origin. IR caused nonlinear dose-dependent effects on secretion of the proinflammatory cytokines KC, MCP-1, and RANTES. The monocyte adhesion was significantly enhanced after IR as well as activation. Conclusions. The study shows that LD-RT, also using very low radiation doses, has a clear immunomodulatory effect on EC as major participants and regulators of inflammation.http://dx.doi.org/10.1155/2018/2856518 |
| spellingShingle | Sabine Schröder Stephan Kriesen Daniel Paape Guido Hildebrandt Katrin Manda Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions Journal of Immunology Research |
| title | Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions |
| title_full | Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions |
| title_fullStr | Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions |
| title_full_unstemmed | Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions |
| title_short | Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions |
| title_sort | modulation of inflammatory reactions by low dose ionizing radiation cytokine release of murine endothelial cells is dependent on culture conditions |
| url | http://dx.doi.org/10.1155/2018/2856518 |
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