Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis
Objective. Hemophilic arthropathy is characterized by recurrent bleeding episodes in patients with hemophilia leading to irreversible joint degeneration. The involvement of CX3CL1 (fractalkine) and its receptor CX3CR1 was observed in the pathogenesis of numerous arthritis-associated diseases. Taking...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/2932696 |
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| author | Piotr Wojdasiewicz Łukasz A. Poniatowski Andrzej Kotela Marta Skoda Michał Pyzlak Aleksandra Stangret Ireneusz Kotela Dariusz Szukiewicz |
| author_facet | Piotr Wojdasiewicz Łukasz A. Poniatowski Andrzej Kotela Marta Skoda Michał Pyzlak Aleksandra Stangret Ireneusz Kotela Dariusz Szukiewicz |
| author_sort | Piotr Wojdasiewicz |
| collection | DOAJ |
| description | Objective. Hemophilic arthropathy is characterized by recurrent bleeding episodes in patients with hemophilia leading to irreversible joint degeneration. The involvement of CX3CL1 (fractalkine) and its receptor CX3CR1 was observed in the pathogenesis of numerous arthritis-associated diseases. Taking this into account, we have presented a study investigating the role of the CX3CL1/CX3XR1 axis in the course of hemophilic arthropathy, including the CX3CL1-dependent expression of CD56+, CD68+, and CD31+ cells along with evaluation of articular cartilage and synovial membrane morphology. Methods. The study was carried out using cases (n=20) of end-stage hemophilic arthropathy with a severe type of hemophilia A and control cases (n=20) diagnosed with osteoarthritis. The biofluids including blood serum and synovial fluid were obtained intraoperatively for the evaluation of CX3CL1 using the ELISA test. Tissue specimens including articular cartilage and synovial membrane were similarly collected during surgery and stained immunohistologically using selected antibodies including anti-CX3CR1, anti-CD56, anti-CD68, and anti-CD31. Additionally, the analysis included the assessment of articular cartilage, synovial membrane, and blood vessel morphology. Results. In our study, we have documented increased average concentration of CX3CL1 in the blood serum of the study group (7.16±0.53 ng/ml) compared to the control group (5.85±0.70 ng/ml) without statistically significant difference in synovial fluid concentration at the same time. We have observed an increased macrophage presence with more marked proliferation and fibrosis of the synovial membrane in the study group. Remaining results such as expression of CX3CR1 presence of NK cells and larger surface area of blood vessels within the synovial membrane were noted also without statistical significance. Conclusions. This study has demonstrated collective CX3CL1/CX3CR1 axis involvement in hemophilic arthropathy pathogenesis introducing new interesting diagnostics and a therapeutic target. |
| format | Article |
| id | doaj-art-e30ee5988e3c4452b481aecb402f364b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e30ee5988e3c4452b481aecb402f364b2025-02-03T06:45:48ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/29326962932696Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and OsteoarthritisPiotr Wojdasiewicz0Łukasz A. Poniatowski1Andrzej Kotela2Marta Skoda3Michał Pyzlak4Aleksandra Stangret5Ireneusz Kotela6Dariusz Szukiewicz7Department of General and Experimental Pathology, Centre for Preclinical Research and Technology (CePT), Medical University of Warsaw, Pawińskiego 3C, 02-106 Warsaw, PolandDepartment of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology (CePT), Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, PolandDepartment of Orthopedics and Traumatology, 1st Faculty of Medicine, Medical University of Warsaw, Lindleya 4, 02-005 Warsaw, PolandDepartment of General and Experimental Pathology, Centre for Preclinical Research and Technology (CePT), Medical University of Warsaw, Pawińskiego 3C, 02-106 Warsaw, PolandDepartment of General and Experimental Pathology, Centre for Preclinical Research and Technology (CePT), Medical University of Warsaw, Pawińskiego 3C, 02-106 Warsaw, PolandDepartment of General and Experimental Pathology, Centre for Preclinical Research and Technology (CePT), Medical University of Warsaw, Pawińskiego 3C, 02-106 Warsaw, PolandDepartment of Orthopedics and Traumatology, Central Clinical Hospital of the Ministry of the Interior and Administration, Wołoska 137, 02-507 Warsaw, PolandDepartment of General and Experimental Pathology, Centre for Preclinical Research and Technology (CePT), Medical University of Warsaw, Pawińskiego 3C, 02-106 Warsaw, PolandObjective. Hemophilic arthropathy is characterized by recurrent bleeding episodes in patients with hemophilia leading to irreversible joint degeneration. The involvement of CX3CL1 (fractalkine) and its receptor CX3CR1 was observed in the pathogenesis of numerous arthritis-associated diseases. Taking this into account, we have presented a study investigating the role of the CX3CL1/CX3XR1 axis in the course of hemophilic arthropathy, including the CX3CL1-dependent expression of CD56+, CD68+, and CD31+ cells along with evaluation of articular cartilage and synovial membrane morphology. Methods. The study was carried out using cases (n=20) of end-stage hemophilic arthropathy with a severe type of hemophilia A and control cases (n=20) diagnosed with osteoarthritis. The biofluids including blood serum and synovial fluid were obtained intraoperatively for the evaluation of CX3CL1 using the ELISA test. Tissue specimens including articular cartilage and synovial membrane were similarly collected during surgery and stained immunohistologically using selected antibodies including anti-CX3CR1, anti-CD56, anti-CD68, and anti-CD31. Additionally, the analysis included the assessment of articular cartilage, synovial membrane, and blood vessel morphology. Results. In our study, we have documented increased average concentration of CX3CL1 in the blood serum of the study group (7.16±0.53 ng/ml) compared to the control group (5.85±0.70 ng/ml) without statistically significant difference in synovial fluid concentration at the same time. We have observed an increased macrophage presence with more marked proliferation and fibrosis of the synovial membrane in the study group. Remaining results such as expression of CX3CR1 presence of NK cells and larger surface area of blood vessels within the synovial membrane were noted also without statistical significance. Conclusions. This study has demonstrated collective CX3CL1/CX3CR1 axis involvement in hemophilic arthropathy pathogenesis introducing new interesting diagnostics and a therapeutic target.http://dx.doi.org/10.1155/2020/2932696 |
| spellingShingle | Piotr Wojdasiewicz Łukasz A. Poniatowski Andrzej Kotela Marta Skoda Michał Pyzlak Aleksandra Stangret Ireneusz Kotela Dariusz Szukiewicz Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis Journal of Immunology Research |
| title | Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis |
| title_full | Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis |
| title_fullStr | Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis |
| title_full_unstemmed | Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis |
| title_short | Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis |
| title_sort | comparative analysis of the occurrence and role of cx3cl1 fractalkine and its receptor cx3cr1 in hemophilic arthropathy and osteoarthritis |
| url | http://dx.doi.org/10.1155/2020/2932696 |
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