Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice
The present work is devoted to the study of pharmacokinetics of infrared photosensitizer (PS) based on hydroxyaluminium tetra‑3‑phenylthiophthalocyanine in a sterically stabilized liposomal form. The study was carried out on adult female mice. The PS was administered once intravenously at a dose of...
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| Format: | Article |
| Language: | Russian |
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Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"
2019-01-01
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| Series: | Biomedical Photonics |
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| Online Access: | https://www.pdt-journal.com/jour/article/view/271 |
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| author | A. P. Budko Z. G. Deichman G. A. Meerovich L. M. Borisova I. G. Мeerovich A. V. Lantsova Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. |
| author_facet | A. P. Budko Z. G. Deichman G. A. Meerovich L. M. Borisova I. G. Мeerovich A. V. Lantsova Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. |
| author_sort | A. P. Budko |
| collection | DOAJ |
| description | The present work is devoted to the study of pharmacokinetics of infrared photosensitizer (PS) based on hydroxyaluminium tetra‑3‑phenylthiophthalocyanine in a sterically stabilized liposomal form. The study was carried out on adult female mice. The PS was administered once intravenously at a dose of 6 mg / kg. Evaluation of the PS accumulation dynamics in the mice tissues and organs was performed at time intervals from 5 minutes to 7 days using spectral‑fluorescent method. The maximum accumulation of the PS photoactive form was recorded in lungs (32 µg / g in the interval of 5–30 minutes after introduction), liver (20.8 µg / g in the interval of 4–24 hours after introduction) and spleen (28 µg / g 4 hours after introduction). At the same time, by the end of the observation period (7 days after administration), trace amounts of the PS photoactive form were still detected in the liver and the spleen at a calculated concentration of 0.5‑1 µg / g. The PS accumulated the least in muscles and skin. The fluorescent signal from the PS accumulated in skin was detectable almost immediately, and its concentration remained at the same level (1.2‑1.5 µg / g) for up to 3 days of observation. In the muscles, the concentration of the PS reached 1.5 µg / g 15 minutes after administration, and then gradually decreased until 0.25 µg / g at 24 hours. Data on the pharmacokinetics of PS in blood, basic organs and tissues of animals were obtained, pharmacokinetic parameters were calculated. 7 days after the administration, the PS concentration in the skin and muscles was below the detection limit. The studies confrmed that PEGylation of the PS liposomal form slows down the process of its capture by reticulo‑endothelial system. It was shown that the PS circulates in blood and organs of mice for a long time and it completely distributes only when 4 hours pass after administration. |
| format | Article |
| id | doaj-art-e2fe4b2ea66e4ea4aef285762f16a300 |
| institution | DOAJ |
| issn | 2413-9432 |
| language | Russian |
| publishDate | 2019-01-01 |
| publisher | Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association" |
| record_format | Article |
| series | Biomedical Photonics |
| spelling | doaj-art-e2fe4b2ea66e4ea4aef285762f16a3002025-08-20T03:00:53ZrusNon-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"Biomedical Photonics2413-94322019-01-0174162210.24931/2413-9432-2018-7-4-16-22151Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on miceA. P. Budko0Z. G. Deichman1G. A. Meerovich2L. M. Borisova3I. G. Мeerovich4A. V. Lantsova5Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. Kulbachevskaya N.Yu.6N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, Moscow, Russia of the Ministry of Health of the Russian Federation, MoscowN.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, Moscow, Russia of the Ministry of Health of the Russian Federation, MoscowProkhorov General Physics Institute of the Russian Academy of Sciences, Moscow; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), MoscowN.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, Moscow, Russia of the Ministry of Health of the Russian Federation, MoscowBach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, MoscowN.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, Moscow, Russia of the Ministry of Health of the Russian Federation, MoscowN.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, Moscow, Russia of the Ministry of Health of the Russian Federation, MoscowThe present work is devoted to the study of pharmacokinetics of infrared photosensitizer (PS) based on hydroxyaluminium tetra‑3‑phenylthiophthalocyanine in a sterically stabilized liposomal form. The study was carried out on adult female mice. The PS was administered once intravenously at a dose of 6 mg / kg. Evaluation of the PS accumulation dynamics in the mice tissues and organs was performed at time intervals from 5 minutes to 7 days using spectral‑fluorescent method. The maximum accumulation of the PS photoactive form was recorded in lungs (32 µg / g in the interval of 5–30 minutes after introduction), liver (20.8 µg / g in the interval of 4–24 hours after introduction) and spleen (28 µg / g 4 hours after introduction). At the same time, by the end of the observation period (7 days after administration), trace amounts of the PS photoactive form were still detected in the liver and the spleen at a calculated concentration of 0.5‑1 µg / g. The PS accumulated the least in muscles and skin. The fluorescent signal from the PS accumulated in skin was detectable almost immediately, and its concentration remained at the same level (1.2‑1.5 µg / g) for up to 3 days of observation. In the muscles, the concentration of the PS reached 1.5 µg / g 15 minutes after administration, and then gradually decreased until 0.25 µg / g at 24 hours. Data on the pharmacokinetics of PS in blood, basic organs and tissues of animals were obtained, pharmacokinetic parameters were calculated. 7 days after the administration, the PS concentration in the skin and muscles was below the detection limit. The studies confrmed that PEGylation of the PS liposomal form slows down the process of its capture by reticulo‑endothelial system. It was shown that the PS circulates in blood and organs of mice for a long time and it completely distributes only when 4 hours pass after administration.https://www.pdt-journal.com/jour/article/view/271photosensitizerpharmacokineticsfluorescence. |
| spellingShingle | A. P. Budko Z. G. Deichman G. A. Meerovich L. M. Borisova I. G. Мeerovich A. V. Lantsova Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice Biomedical Photonics photosensitizer pharmacokinetics fluorescence. |
| title | Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice |
| title_full | Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice |
| title_fullStr | Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice |
| title_full_unstemmed | Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice |
| title_short | Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice |
| title_sort | study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra 3 phenylthiophthalocyanine on mice |
| topic | photosensitizer pharmacokinetics fluorescence. |
| url | https://www.pdt-journal.com/jour/article/view/271 |
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