Effects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men
Plant-based diets that replace animal-based proteins with plant-based proteins have received increased attention for cardiovascular protection. Nitric oxide (NO) plays an essential role in the maintenance of endothelial function. However, under higher oxidative stress, NO generation produces peroxyn...
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| Format: | Article |
| Language: | English |
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The Japan Endocrine Society
2024-02-01
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| Series: | Endocrine Journal |
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| Online Access: | https://www.jstage.jst.go.jp/article/endocrj/71/2/71_EJ23-0355/_html/-char/en |
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| author | Tomoko Kaneko Masaki Yoshioka Futo Kawahara Natsumi Nishitani Shoya Mori Jiyeon Park Takashi Tarumi Keisei Kosaki Seiji Maeda |
| author_facet | Tomoko Kaneko Masaki Yoshioka Futo Kawahara Natsumi Nishitani Shoya Mori Jiyeon Park Takashi Tarumi Keisei Kosaki Seiji Maeda |
| author_sort | Tomoko Kaneko |
| collection | DOAJ |
| description | Plant-based diets that replace animal-based proteins with plant-based proteins have received increased attention for cardiovascular protection. Nitric oxide (NO) plays an essential role in the maintenance of endothelial function. However, under higher oxidative stress, NO generation produces peroxynitrite, a powerful oxidant and vasoconstrictor. Diet-replaced protein sources has been reported to decrease oxidative stress. However, the effects of plant-based protein on NO and peroxynitrite have not yet been clarified. Therefore, this study aimed to compare the effects of plant- and animal-based-protein meals for a day on NO, peroxynitrite, and NO/peroxynitrite balance. A crossover trial of two meal conditions involving nine healthy men was performed. Participants ate standard meals during day 1. On day 2, baseline measurements were performed and the participants were provided with plant-based-protein meals or animal-based-protein meals. The standard and test meals consisted of breakfast, lunch, and dinner and were designed to be isocaloric. Plant-based-protein meals contained no animal protein. Blood samples were collected in the morning after overnight fasting before and after the test meals consumption. In the plant-based-protein meal condition, serum NOx levels (the sum of serum nitrite and nitrate) significantly increased, while serum peroxynitrite levels did not change significantly. Animal-based-protein meals significantly increased serum peroxynitrite levels but showed a trend of reduction in the serum NOx levels. Furthermore, serum NO/peroxynitrite balance significantly increased after plant-based-protein meals consumption, but significantly decreased after animal-based-protein meals consumption. These results suggest that, compared with animal-based-protein meals, plant-based-protein meals increase NO levels and NO/peroxynitrite balance, which reflects increased endothelial function. |
| format | Article |
| id | doaj-art-e2f861f0eee64e8491e6123e2108e4a6 |
| institution | Kabale University |
| issn | 1348-4540 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | The Japan Endocrine Society |
| record_format | Article |
| series | Endocrine Journal |
| spelling | doaj-art-e2f861f0eee64e8491e6123e2108e4a62025-01-22T06:33:21ZengThe Japan Endocrine SocietyEndocrine Journal1348-45402024-02-0171211912710.1507/endocrj.EJ23-0355endocrjEffects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young menTomoko Kaneko0Masaki Yoshioka1Futo Kawahara2Natsumi Nishitani3Shoya Mori4Jiyeon Park5Takashi Tarumi6Keisei Kosaki7Seiji Maeda8Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8574, JapanGraduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8574, JapanGraduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8574, JapanGraduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8574, JapanInstitute of Health and Sport Sciences, University of Tsukuba, Ibaraki 305-8574, JapanInstitute of Health and Sport Sciences, University of Tsukuba, Ibaraki 305-8574, JapanGraduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8574, JapanInstitute of Health and Sport Sciences, University of Tsukuba, Ibaraki 305-8574, JapanInstitute of Health and Sport Sciences, University of Tsukuba, Ibaraki 305-8574, JapanPlant-based diets that replace animal-based proteins with plant-based proteins have received increased attention for cardiovascular protection. Nitric oxide (NO) plays an essential role in the maintenance of endothelial function. However, under higher oxidative stress, NO generation produces peroxynitrite, a powerful oxidant and vasoconstrictor. Diet-replaced protein sources has been reported to decrease oxidative stress. However, the effects of plant-based protein on NO and peroxynitrite have not yet been clarified. Therefore, this study aimed to compare the effects of plant- and animal-based-protein meals for a day on NO, peroxynitrite, and NO/peroxynitrite balance. A crossover trial of two meal conditions involving nine healthy men was performed. Participants ate standard meals during day 1. On day 2, baseline measurements were performed and the participants were provided with plant-based-protein meals or animal-based-protein meals. The standard and test meals consisted of breakfast, lunch, and dinner and were designed to be isocaloric. Plant-based-protein meals contained no animal protein. Blood samples were collected in the morning after overnight fasting before and after the test meals consumption. In the plant-based-protein meal condition, serum NOx levels (the sum of serum nitrite and nitrate) significantly increased, while serum peroxynitrite levels did not change significantly. Animal-based-protein meals significantly increased serum peroxynitrite levels but showed a trend of reduction in the serum NOx levels. Furthermore, serum NO/peroxynitrite balance significantly increased after plant-based-protein meals consumption, but significantly decreased after animal-based-protein meals consumption. These results suggest that, compared with animal-based-protein meals, plant-based-protein meals increase NO levels and NO/peroxynitrite balance, which reflects increased endothelial function.https://www.jstage.jst.go.jp/article/endocrj/71/2/71_EJ23-0355/_html/-char/enplant-based proteinnitric oxideperoxynitriteendothelial functioncardiovascular disease |
| spellingShingle | Tomoko Kaneko Masaki Yoshioka Futo Kawahara Natsumi Nishitani Shoya Mori Jiyeon Park Takashi Tarumi Keisei Kosaki Seiji Maeda Effects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men Endocrine Journal plant-based protein nitric oxide peroxynitrite endothelial function cardiovascular disease |
| title | Effects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men |
| title_full | Effects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men |
| title_fullStr | Effects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men |
| title_full_unstemmed | Effects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men |
| title_short | Effects of plant- and animal-based-protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men |
| title_sort | effects of plant and animal based protein meals for a day on serum nitric oxide and peroxynitrite levels in healthy young men |
| topic | plant-based protein nitric oxide peroxynitrite endothelial function cardiovascular disease |
| url | https://www.jstage.jst.go.jp/article/endocrj/71/2/71_EJ23-0355/_html/-char/en |
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