Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075
γ-Aminobutyric acid (GABA) is a valuable amino acid widely used in food, healthcare, and agriculture. GABA bioproduction by budding yeasts has been commonly reported, but related studies using non-conventional yeasts remain limited. In this study, two non-conventional natural yeast strains, namely,...
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2024-12-01
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| author | Ting-Ting Fan Chao Chen Du-Wen Zeng Feng-Lou Wang Zhao-Xian Xu Ming-Jie Jin Yue Zou Jun Li Xin-Qing Zhao |
| author_facet | Ting-Ting Fan Chao Chen Du-Wen Zeng Feng-Lou Wang Zhao-Xian Xu Ming-Jie Jin Yue Zou Jun Li Xin-Qing Zhao |
| author_sort | Ting-Ting Fan |
| collection | DOAJ |
| description | γ-Aminobutyric acid (GABA) is a valuable amino acid widely used in food, healthcare, and agriculture. GABA bioproduction by budding yeasts has been commonly reported, but related studies using non-conventional yeasts remain limited. In this study, two non-conventional natural yeast strains, namely, <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075, were identified as promising GABA producers, and <i>M. reukaufii</i> JMY075 was discovered to be a GABA producer. Enhanced GABA production was observed in the two yeast strains under stress conditions, including high temperature and high ethanol and acetic acid levels. In particular, <i>K. marxianus</i> JMY140K showed 7.93 times higher GABA titers under thermal stress than that of the control. External stress conditions significantly influenced the GABA production of these two yeast strains. The culture filtrate of <i>K. marxianus</i> JMY140K also showed promising activities in human skin cells. In addition, <i>K. marxianus</i> JMY140K could also produce GABA using rice straw hydrolysate, which indicated that it has the potential to produce GABA using renewable biomass. Our studies provide insight for further enhancing the GABA production of natural yeasts and promoting its biotechnology applications. |
| format | Article |
| id | doaj-art-e2ed3d31a8304f19b128788d9efb2251 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj-art-e2ed3d31a8304f19b128788d9efb22512025-01-24T13:37:15ZengMDPI AGJournal of Fungi2309-608X2024-12-011112010.3390/jof11010020Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075Ting-Ting Fan0Chao Chen1Du-Wen Zeng2Feng-Lou Wang3Zhao-Xian Xu4Ming-Jie Jin5Yue Zou6Jun Li7Xin-Qing Zhao8State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaCollege of Life Science, Dalian Minzu University, Dalian 116600, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaR&D Center, Shanghai CHANDO Group Co., Ltd., Shanghai 200233, ChinaSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaR&D Center, Shanghai CHANDO Group Co., Ltd., Shanghai 200233, ChinaR&D Center, Shanghai CHANDO Group Co., Ltd., Shanghai 200233, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, Chinaγ-Aminobutyric acid (GABA) is a valuable amino acid widely used in food, healthcare, and agriculture. GABA bioproduction by budding yeasts has been commonly reported, but related studies using non-conventional yeasts remain limited. In this study, two non-conventional natural yeast strains, namely, <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075, were identified as promising GABA producers, and <i>M. reukaufii</i> JMY075 was discovered to be a GABA producer. Enhanced GABA production was observed in the two yeast strains under stress conditions, including high temperature and high ethanol and acetic acid levels. In particular, <i>K. marxianus</i> JMY140K showed 7.93 times higher GABA titers under thermal stress than that of the control. External stress conditions significantly influenced the GABA production of these two yeast strains. The culture filtrate of <i>K. marxianus</i> JMY140K also showed promising activities in human skin cells. In addition, <i>K. marxianus</i> JMY140K could also produce GABA using rice straw hydrolysate, which indicated that it has the potential to produce GABA using renewable biomass. Our studies provide insight for further enhancing the GABA production of natural yeasts and promoting its biotechnology applications.https://www.mdpi.com/2309-608X/11/1/20non-conventional yeast<i>K. marxianus</i><i>M. reukaufii</i>γ-aminobutyric acid productionstress stimulation |
| spellingShingle | Ting-Ting Fan Chao Chen Du-Wen Zeng Feng-Lou Wang Zhao-Xian Xu Ming-Jie Jin Yue Zou Jun Li Xin-Qing Zhao Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075 Journal of Fungi non-conventional yeast <i>K. marxianus</i> <i>M. reukaufii</i> γ-aminobutyric acid production stress stimulation |
| title | Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075 |
| title_full | Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075 |
| title_fullStr | Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075 |
| title_full_unstemmed | Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075 |
| title_short | Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075 |
| title_sort | stress driven production of γ aminobutyric acid using non conventional yeast strains i kluyveromyces marxianus i jmy140k and i metschnikowia reukaufii i jmy075 |
| topic | non-conventional yeast <i>K. marxianus</i> <i>M. reukaufii</i> γ-aminobutyric acid production stress stimulation |
| url | https://www.mdpi.com/2309-608X/11/1/20 |
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