What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from Brazil
The rise of antibiotic resistance is a growing challenge, affecting humans, the environment, and animals. Under the One Health framework, this study investigated resistance mechanisms to critically important antibiotics in frozen chicken nuggets imported from Brazil.Eighty nugget samples were cultur...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | One Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352771425002071 |
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| author | Nicolás F. Cordeiro Nadia Coppola Federica Ferreira Rafael Vignoli Inés Bado |
| author_facet | Nicolás F. Cordeiro Nadia Coppola Federica Ferreira Rafael Vignoli Inés Bado |
| author_sort | Nicolás F. Cordeiro |
| collection | DOAJ |
| description | The rise of antibiotic resistance is a growing challenge, affecting humans, the environment, and animals. Under the One Health framework, this study investigated resistance mechanisms to critically important antibiotics in frozen chicken nuggets imported from Brazil.Eighty nugget samples were cultured on selective media containing ceftriaxone, ciprofloxacin, or colistin. Isolates were identified using MALDI-TOF, and antibiotic susceptibility was assessed by disk diffusion. Eight samples were also analyzed using shotgun metagenomic sequencing processed through the SqueezeMeta pipeline.Nineteen Enterobacterales resistant mainly to β-lactams and to a lesser extent, to quinolones and aminoglycosides, were identified. Eight Pseudomonas spp. were recovered, including one P. fulva resistant to colistin. Metagenomics revealed predominant Firmicutes, (Bacillaceae, Lactobacillaceae, and Paenibacillaceae) with low γ-Proteobacteria levels.Additionally, we detected resistance genes against several antibiotics.This study highlights the role of imported food in spreading AMR and the value of combining metagenomics with conventional microbiology to strengthen One Health surveillance. |
| format | Article |
| id | doaj-art-e2e2d391c3af4a81b894414bc92600a8 |
| institution | Kabale University |
| issn | 2352-7714 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | One Health |
| spelling | doaj-art-e2e2d391c3af4a81b894414bc92600a82025-08-25T04:14:35ZengElsevierOne Health2352-77142025-12-012110117110.1016/j.onehlt.2025.101171What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from BrazilNicolás F. Cordeiro0Nadia Coppola1Federica Ferreira2Rafael Vignoli3Inés Bado4Departamento de Bacteriología y Virología, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Av. Alfredo Navarro 3051, CP. 11600 Montevideo, UruguayDepartamento de Bacteriología y Virología, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Av. Alfredo Navarro 3051, CP. 11600 Montevideo, UruguayDepartamento de Bacteriología y Virología, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Av. Alfredo Navarro 3051, CP. 11600 Montevideo, UruguayDepartamento de Bacteriología y Virología, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Av. Alfredo Navarro 3051, CP. 11600 Montevideo, UruguayCorresponding author.; Departamento de Bacteriología y Virología, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Av. Alfredo Navarro 3051, CP. 11600 Montevideo, UruguayThe rise of antibiotic resistance is a growing challenge, affecting humans, the environment, and animals. Under the One Health framework, this study investigated resistance mechanisms to critically important antibiotics in frozen chicken nuggets imported from Brazil.Eighty nugget samples were cultured on selective media containing ceftriaxone, ciprofloxacin, or colistin. Isolates were identified using MALDI-TOF, and antibiotic susceptibility was assessed by disk diffusion. Eight samples were also analyzed using shotgun metagenomic sequencing processed through the SqueezeMeta pipeline.Nineteen Enterobacterales resistant mainly to β-lactams and to a lesser extent, to quinolones and aminoglycosides, were identified. Eight Pseudomonas spp. were recovered, including one P. fulva resistant to colistin. Metagenomics revealed predominant Firmicutes, (Bacillaceae, Lactobacillaceae, and Paenibacillaceae) with low γ-Proteobacteria levels.Additionally, we detected resistance genes against several antibiotics.This study highlights the role of imported food in spreading AMR and the value of combining metagenomics with conventional microbiology to strengthen One Health surveillance.http://www.sciencedirect.com/science/article/pii/S2352771425002071NuggetsFrozen foodAntibiotic resistanceMetagenomicsOne health |
| spellingShingle | Nicolás F. Cordeiro Nadia Coppola Federica Ferreira Rafael Vignoli Inés Bado What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from Brazil One Health Nuggets Frozen food Antibiotic resistance Metagenomics One health |
| title | What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from Brazil |
| title_full | What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from Brazil |
| title_fullStr | What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from Brazil |
| title_full_unstemmed | What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from Brazil |
| title_short | What are we eating?. Detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from Brazil |
| title_sort | what are we eating detection of antibiotic resistance mechanisms in frozen chicken nuggets imported from brazil |
| topic | Nuggets Frozen food Antibiotic resistance Metagenomics One health |
| url | http://www.sciencedirect.com/science/article/pii/S2352771425002071 |
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