Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer
Colorectal cancer (CRC) is a prevalent malignant tumor worldwide, with a high mortality rate due to its complex etiology and limited early screening techniques. This study aimed to identify potential biomarkers for early detection of CRC utilizing targeted metabolite profiling of platelet‐rich plasm...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | Molecular Oncology |
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| Online Access: | https://doi.org/10.1002/1878-0261.13791 |
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| author | Zuojian Hu Fenglin Shen Yang Liu Ziqing Zhong Yongling Chen Zhiyuan Xia Cuiju Mo Hongxiu Yu |
| author_facet | Zuojian Hu Fenglin Shen Yang Liu Ziqing Zhong Yongling Chen Zhiyuan Xia Cuiju Mo Hongxiu Yu |
| author_sort | Zuojian Hu |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is a prevalent malignant tumor worldwide, with a high mortality rate due to its complex etiology and limited early screening techniques. This study aimed to identify potential biomarkers for early detection of CRC utilizing targeted metabolite profiling of platelet‐rich plasma (PRP). Based on multiple reaction monitoring (MRM) mode, liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis identified metabolites in PRP collected from patients with CRC (n = 70) and healthy controls (n = 30). A total of 302 metabolites were identified and quantified in this study, including various categories such as lipids, lipid mediators, amino acids, and derivatives, organic acids and derivatives, nucleotides and derivatives, alkaloids, carbohydrates, vitamins and derivatives, and others. The differential analysis revealed that five carbohydrates and organic acids (lactose, glycerol‐3‐phosphate, 2−hydroxyglutaric acid, isocitric acid, and citric acid) involved in the carbohydrate metabolism pathway displayed consistent upregulation within PRP derived from patients with CRC. To further validate the abundance of differential metabolites, 10 pairs of CRC tissues, adjacent tissues, and matched PRP were collected. Ultimately, five carbohydrate metabolites were validated in PRP, and compared with carcinoembryonic antigen (CEA) and cancer antigen 19‐9 (CA199), the five carbohydrate metabolites significantly improved the specificity of differentiating patients with CRC from healthy controls. Furthermore, the diagnostic efficacy of the combined five‐carbohydrate metabolite panel was superior to that of individual metabolites, CEA and CA199. The sensitivity, specificity, and AUC of the metabolite panel in distinguishing patients with CRC from healthy controls were 90.00%, 96.67%, and 0.961 (95% CI 0.922–0.998), respectively. Collectively, metabolomics was used to identify and validate differential metabolites in the PRP of CRC, which may serve as potential early screening markers for patients with CRC. |
| format | Article |
| id | doaj-art-e2d0ff9d37c44a3895c88b2bcaa28e71 |
| institution | Kabale University |
| issn | 1574-7891 1878-0261 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Oncology |
| spelling | doaj-art-e2d0ff9d37c44a3895c88b2bcaa28e712025-08-20T03:25:33ZengWileyMolecular Oncology1574-78911878-02612025-06-011961737175010.1002/1878-0261.13791Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancerZuojian Hu0Fenglin Shen1Yang Liu2Ziqing Zhong3Yongling Chen4Zhiyuan Xia5Cuiju Mo6Hongxiu Yu7Shanghai Stomatological Hospital & School of Stomatology & Institutes of Biomedical Sciences Fudan University Shanghai ChinaShanghai Stomatological Hospital & School of Stomatology & Institutes of Biomedical Sciences Fudan University Shanghai ChinaShanghai Stomatological Hospital & School of Stomatology & Institutes of Biomedical Sciences Fudan University Shanghai ChinaDepartment of Clinical Laboratory First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Clinical Laboratory First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Colorectal & Anal Surgery First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Clinical Laboratory First Affiliated Hospital of Guangxi Medical University Nanning ChinaShanghai Stomatological Hospital & School of Stomatology & Institutes of Biomedical Sciences Fudan University Shanghai ChinaColorectal cancer (CRC) is a prevalent malignant tumor worldwide, with a high mortality rate due to its complex etiology and limited early screening techniques. This study aimed to identify potential biomarkers for early detection of CRC utilizing targeted metabolite profiling of platelet‐rich plasma (PRP). Based on multiple reaction monitoring (MRM) mode, liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis identified metabolites in PRP collected from patients with CRC (n = 70) and healthy controls (n = 30). A total of 302 metabolites were identified and quantified in this study, including various categories such as lipids, lipid mediators, amino acids, and derivatives, organic acids and derivatives, nucleotides and derivatives, alkaloids, carbohydrates, vitamins and derivatives, and others. The differential analysis revealed that five carbohydrates and organic acids (lactose, glycerol‐3‐phosphate, 2−hydroxyglutaric acid, isocitric acid, and citric acid) involved in the carbohydrate metabolism pathway displayed consistent upregulation within PRP derived from patients with CRC. To further validate the abundance of differential metabolites, 10 pairs of CRC tissues, adjacent tissues, and matched PRP were collected. Ultimately, five carbohydrate metabolites were validated in PRP, and compared with carcinoembryonic antigen (CEA) and cancer antigen 19‐9 (CA199), the five carbohydrate metabolites significantly improved the specificity of differentiating patients with CRC from healthy controls. Furthermore, the diagnostic efficacy of the combined five‐carbohydrate metabolite panel was superior to that of individual metabolites, CEA and CA199. The sensitivity, specificity, and AUC of the metabolite panel in distinguishing patients with CRC from healthy controls were 90.00%, 96.67%, and 0.961 (95% CI 0.922–0.998), respectively. Collectively, metabolomics was used to identify and validate differential metabolites in the PRP of CRC, which may serve as potential early screening markers for patients with CRC.https://doi.org/10.1002/1878-0261.13791carbohydrate metabolitescolorectal cancerdiagnostic modelmetabolomicsplatelet rich plasma |
| spellingShingle | Zuojian Hu Fenglin Shen Yang Liu Ziqing Zhong Yongling Chen Zhiyuan Xia Cuiju Mo Hongxiu Yu Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer Molecular Oncology carbohydrate metabolites colorectal cancer diagnostic model metabolomics platelet rich plasma |
| title | Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer |
| title_full | Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer |
| title_fullStr | Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer |
| title_full_unstemmed | Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer |
| title_short | Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer |
| title_sort | targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer |
| topic | carbohydrate metabolites colorectal cancer diagnostic model metabolomics platelet rich plasma |
| url | https://doi.org/10.1002/1878-0261.13791 |
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