Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance
ABSTRACT Bioelectrochemical systems (BESs) offer a sustainable method for chemical production, including the enhanced production of succinic acid. By combining fermentation with BES, it could be possible to achieve sustainable succinic acid production and CO2 fixation using Actinobacillus succinogen...
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Wiley-VCH
2025-01-01
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| Series: | Engineering in Life Sciences |
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| Online Access: | https://doi.org/10.1002/elsc.202400053 |
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| author | Jan‐Niklas Hengsbach Marcel Cwienczek Wolfgang Laudensack Judith Stiefelmaier Nils Tippkötter Roland Ulber |
| author_facet | Jan‐Niklas Hengsbach Marcel Cwienczek Wolfgang Laudensack Judith Stiefelmaier Nils Tippkötter Roland Ulber |
| author_sort | Jan‐Niklas Hengsbach |
| collection | DOAJ |
| description | ABSTRACT Bioelectrochemical systems (BESs) offer a sustainable method for chemical production, including the enhanced production of succinic acid. By combining fermentation with BES, it could be possible to achieve sustainable succinic acid production and CO2 fixation using Actinobacillus succinogenes. In literature, the potential application of BES is commonly associated with increased succinate yields, as it is expected to enhance the availability of NADH, thereby influencing the intracellular nicotinamide adenine dinucleotide (NADH/NAD+) balance. However, it remains unclear whether BES can improve NADH regeneration and achieve higher NADH/NAD+ ratios across all growth phases of A. succinogenes. This study investigates the impact of an applied electrical potential on the intracellular NADH/NAD+ ratio during an electrochemical‐assisted fermentation process. Using an adapted high‐performance liquid chromatography method with a Supelcosil LC‐18‐T column, it was demonstrated that NADH availability in BES, particularly during the stationary growth phase, improved by up to 1.98‐fold compared to the control. This enhancement in reducing power led to a succinate yield of 0.747 ± 0.01 g g−1, representing a 15.65% increase compared to a fermentation without electrochemical assistance. These findings support the expectation that the use of BES could enhance the competitiveness of bio‐based succinate production. |
| format | Article |
| id | doaj-art-e2cd5c6321144d2791b29f88b68f9f68 |
| institution | Kabale University |
| issn | 1618-0240 1618-2863 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj-art-e2cd5c6321144d2791b29f88b68f9f682025-01-30T06:40:30ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632025-01-01251n/an/a10.1002/elsc.202400053Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ BalanceJan‐Niklas Hengsbach0Marcel Cwienczek1Wolfgang Laudensack2Judith Stiefelmaier3Nils Tippkötter4Roland Ulber5Mechanical and Process Engineering RPTU Kaiserslautern‐Landau Kaiserslautern GermanyMechanical and Process Engineering RPTU Kaiserslautern‐Landau Kaiserslautern GermanyMechanical and Process Engineering RPTU Kaiserslautern‐Landau Kaiserslautern GermanyMechanical and Process Engineering RPTU Kaiserslautern‐Landau Kaiserslautern GermanyBioprocess Engineering and Downstream Processing University of Applied Science Aachen Jülich GermanyMechanical and Process Engineering RPTU Kaiserslautern‐Landau Kaiserslautern GermanyABSTRACT Bioelectrochemical systems (BESs) offer a sustainable method for chemical production, including the enhanced production of succinic acid. By combining fermentation with BES, it could be possible to achieve sustainable succinic acid production and CO2 fixation using Actinobacillus succinogenes. In literature, the potential application of BES is commonly associated with increased succinate yields, as it is expected to enhance the availability of NADH, thereby influencing the intracellular nicotinamide adenine dinucleotide (NADH/NAD+) balance. However, it remains unclear whether BES can improve NADH regeneration and achieve higher NADH/NAD+ ratios across all growth phases of A. succinogenes. This study investigates the impact of an applied electrical potential on the intracellular NADH/NAD+ ratio during an electrochemical‐assisted fermentation process. Using an adapted high‐performance liquid chromatography method with a Supelcosil LC‐18‐T column, it was demonstrated that NADH availability in BES, particularly during the stationary growth phase, improved by up to 1.98‐fold compared to the control. This enhancement in reducing power led to a succinate yield of 0.747 ± 0.01 g g−1, representing a 15.65% increase compared to a fermentation without electrochemical assistance. These findings support the expectation that the use of BES could enhance the competitiveness of bio‐based succinate production.https://doi.org/10.1002/elsc.202400053Actinobacillus succinogenesbioelectrochemical systemelectrobiotechnologyNADH/NAD+succinic acid |
| spellingShingle | Jan‐Niklas Hengsbach Marcel Cwienczek Wolfgang Laudensack Judith Stiefelmaier Nils Tippkötter Roland Ulber Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance Engineering in Life Sciences Actinobacillus succinogenes bioelectrochemical system electrobiotechnology NADH/NAD+ succinic acid |
| title | Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance |
| title_full | Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance |
| title_fullStr | Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance |
| title_full_unstemmed | Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance |
| title_short | Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance |
| title_sort | succinic acid production with actinobacillus succinogenes influence of an electric potential on the intercellular nadh nad balance |
| topic | Actinobacillus succinogenes bioelectrochemical system electrobiotechnology NADH/NAD+ succinic acid |
| url | https://doi.org/10.1002/elsc.202400053 |
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