A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Bioactive ceramics have been used in bone tissue repair and regeneration. However, because of the complex in vivo osteogenesis process, long cycle, and difficulty of accurately tracking, the mechanism of interaction between materials and cells has yet to be fully understood, hindering its developmen...
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24004894 |
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| author | Sheng Ye Quanle Cao Panxianzhi Ni Shuting Xiong Meng Zhong Tun Yuan Jing Shan Jie Liang Yujiang Fan Xingdong Zhang |
| author_facet | Sheng Ye Quanle Cao Panxianzhi Ni Shuting Xiong Meng Zhong Tun Yuan Jing Shan Jie Liang Yujiang Fan Xingdong Zhang |
| author_sort | Sheng Ye |
| collection | DOAJ |
| description | Bioactive ceramics have been used in bone tissue repair and regeneration. However, because of the complex in vivo osteogenesis process, long cycle, and difficulty of accurately tracking, the mechanism of interaction between materials and cells has yet to be fully understood, hindering its development. The ceramic microbridge microfluidic chip system may solve the problem and provide an in vitro method to simulate the microenvironment in vivo. Nevertheless, the complex microenvironment parameters of the chip system need to be studied in detail. Computer simulation bionics can provide clues for the setting of microenvironment parameters. This study used a computational bionic model to simulate the bone growth process in the presence of immune-related factors. The osteoblast differentiation of mesenchymal stem cells of calcium phosphate ceramics in a macrophage-dominated immune microenvironment was studied using a microfluidic chip system. The computational biomimetic model and microfluidic chip findings were basically consistent with the reported results of the animal experiments. These findings suggest that studying the osteogenic behavior of calcium phosphate ceramics using a microfluidic chip model is feasible. The method model provided in this study can be extended to other biomaterials, providing a viable path for their research and evaluation. |
| format | Article |
| id | doaj-art-e2c6f4fe9d3c44fcabb1267fbfe5d899 |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-e2c6f4fe9d3c44fcabb1267fbfe5d8992025-01-26T05:04:19ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-03-0145520533A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironmentSheng Ye0Quanle Cao1Panxianzhi Ni2Shuting Xiong3Meng Zhong4Tun Yuan5Jing Shan6Jie Liang7Yujiang Fan8Xingdong Zhang9College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, China; School of Big Health & Intelligent Engineering, Chengdu Medical College, Chengdu, Sichuan, 610500, China; Nuclear Industry 416 Hospital, the 2nd Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, 610057, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, China; Sichuan Testing Centre for Biomaterials and Medical Devices, Chengdu, Sichuan, 610064, China; Corresponding author. College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, China.Department of Gastroenterology, the 3rd People's Hospital of Chengdu, Southwest Jiaotong University, Chengdu, Sichuan, 610064, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, China; Sichuan Testing Centre for Biomaterials and Medical Devices, Chengdu, Sichuan, 610064, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610064, ChinaBioactive ceramics have been used in bone tissue repair and regeneration. However, because of the complex in vivo osteogenesis process, long cycle, and difficulty of accurately tracking, the mechanism of interaction between materials and cells has yet to be fully understood, hindering its development. The ceramic microbridge microfluidic chip system may solve the problem and provide an in vitro method to simulate the microenvironment in vivo. Nevertheless, the complex microenvironment parameters of the chip system need to be studied in detail. Computer simulation bionics can provide clues for the setting of microenvironment parameters. This study used a computational bionic model to simulate the bone growth process in the presence of immune-related factors. The osteoblast differentiation of mesenchymal stem cells of calcium phosphate ceramics in a macrophage-dominated immune microenvironment was studied using a microfluidic chip system. The computational biomimetic model and microfluidic chip findings were basically consistent with the reported results of the animal experiments. These findings suggest that studying the osteogenic behavior of calcium phosphate ceramics using a microfluidic chip model is feasible. The method model provided in this study can be extended to other biomaterials, providing a viable path for their research and evaluation.http://www.sciencedirect.com/science/article/pii/S2452199X24004894Bioactive ceramicsThe ceramic microbridge microfluidic chip systemComputer simulation bionicsImmune microenvironmentResearch and evaluation |
| spellingShingle | Sheng Ye Quanle Cao Panxianzhi Ni Shuting Xiong Meng Zhong Tun Yuan Jing Shan Jie Liang Yujiang Fan Xingdong Zhang A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment Bioactive Materials Bioactive ceramics The ceramic microbridge microfluidic chip system Computer simulation bionics Immune microenvironment Research and evaluation |
| title | A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment |
| title_full | A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment |
| title_fullStr | A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment |
| title_full_unstemmed | A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment |
| title_short | A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment |
| title_sort | ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment |
| topic | Bioactive ceramics The ceramic microbridge microfluidic chip system Computer simulation bionics Immune microenvironment Research and evaluation |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24004894 |
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