Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi
Inflammatory response in Chagas disease is related to parasite and host factors. However, immune system regulation has not been fully elucidated. Thus, this study is aimed at evaluating IL-4 influence on acute phase of Trypanosoma cruzi experimental infection through dosage of cytokine levels in car...
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| Format: | Article |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/3421897 |
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| author | Marcos Vinicius da Silva Vera Lúcia de Almeida Wendyson Duarte de Oliveira Natália Carasek Matos Cascudo Pollyana Guimarães de Oliveira Crislaine Aparecida da Silva Aline Cristina Souza da Silva Maria Luíza Gonçalves dos Reis Monteiro Rosana Rosa Miranda Correa Milton Adriano Pelli Oliveira Ruy de Sousa Lino-Júnior Mara Rúbia Nunes Celes Juliana Reis Machado |
| author_facet | Marcos Vinicius da Silva Vera Lúcia de Almeida Wendyson Duarte de Oliveira Natália Carasek Matos Cascudo Pollyana Guimarães de Oliveira Crislaine Aparecida da Silva Aline Cristina Souza da Silva Maria Luíza Gonçalves dos Reis Monteiro Rosana Rosa Miranda Correa Milton Adriano Pelli Oliveira Ruy de Sousa Lino-Júnior Mara Rúbia Nunes Celes Juliana Reis Machado |
| author_sort | Marcos Vinicius da Silva |
| collection | DOAJ |
| description | Inflammatory response in Chagas disease is related to parasite and host factors. However, immune system regulation has not been fully elucidated. Thus, this study is aimed at evaluating IL-4 influence on acute phase of Trypanosoma cruzi experimental infection through dosage of cytokine levels in cardiac homogenate of infected Balb/c WT and Balb/c IL-4−/− as well as its histopathological repercussions. For such purpose, mice were divided into two groups: an infected group with 100 forms of the Colombian strain and an uninfected group. After 21 days of infection, animals were euthanized and the blood, spleen, and heart were collected. The spleen was used to culture splenic cells in 48 h. Subsequently, cytokines TNF-α, IL-12p70, IL-10, IFN-γ, and IL-17 were measured in the blood, culture supernatant, and heart apex by ELISA. The base of the heart was used for histopathological analysis. From these analysis, infected Balb/c IL-4−/− mice showed milder inflammatory infiltrate compared to Balb/c WT, but without changes in nest density and collagen deposition. IL-4 absence culminated in lower cardiac tissue IFN-γ production, although it did not affect TNF-α expression in situ. It also decreased TNF-α systemic production and increased IL-10, both systemically and in situ. In addition, IL-4 absence did not influence IL-17 expression. Splenocytes of IL-4-deficient mice produced higher amounts of IFN-γ, TNF-α, and IL-17 and lower amounts of IL-10. Thus, IL-4 absence in acute phase of experimental infection with T. cruzi Colombian strain reduces myocarditis due to lower IFN-γ production and greater IL-10 production in situ and this pattern is not influenced by splenocyte general repertoire. |
| format | Article |
| id | doaj-art-e2c0cd818f9a4cc18f175c6a3883d164 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-e2c0cd818f9a4cc18f175c6a3883d1642025-02-03T01:21:31ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/34218973421897Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruziMarcos Vinicius da Silva0Vera Lúcia de Almeida1Wendyson Duarte de Oliveira2Natália Carasek Matos Cascudo3Pollyana Guimarães de Oliveira4Crislaine Aparecida da Silva5Aline Cristina Souza da Silva6Maria Luíza Gonçalves dos Reis Monteiro7Rosana Rosa Miranda Correa8Milton Adriano Pelli Oliveira9Ruy de Sousa Lino-Júnior10Mara Rúbia Nunes Celes11Juliana Reis Machado12Department of Parasitology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, BrazilInstitute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, BrazilInstitute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, BrazilInstitute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, BrazilInstitute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, BrazilGeneral Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, BrazilGeneral Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, BrazilGeneral Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, BrazilGeneral Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, BrazilInstitute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, BrazilInstitute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, BrazilInstitute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, BrazilGeneral Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, BrazilInflammatory response in Chagas disease is related to parasite and host factors. However, immune system regulation has not been fully elucidated. Thus, this study is aimed at evaluating IL-4 influence on acute phase of Trypanosoma cruzi experimental infection through dosage of cytokine levels in cardiac homogenate of infected Balb/c WT and Balb/c IL-4−/− as well as its histopathological repercussions. For such purpose, mice were divided into two groups: an infected group with 100 forms of the Colombian strain and an uninfected group. After 21 days of infection, animals were euthanized and the blood, spleen, and heart were collected. The spleen was used to culture splenic cells in 48 h. Subsequently, cytokines TNF-α, IL-12p70, IL-10, IFN-γ, and IL-17 were measured in the blood, culture supernatant, and heart apex by ELISA. The base of the heart was used for histopathological analysis. From these analysis, infected Balb/c IL-4−/− mice showed milder inflammatory infiltrate compared to Balb/c WT, but without changes in nest density and collagen deposition. IL-4 absence culminated in lower cardiac tissue IFN-γ production, although it did not affect TNF-α expression in situ. It also decreased TNF-α systemic production and increased IL-10, both systemically and in situ. In addition, IL-4 absence did not influence IL-17 expression. Splenocytes of IL-4-deficient mice produced higher amounts of IFN-γ, TNF-α, and IL-17 and lower amounts of IL-10. Thus, IL-4 absence in acute phase of experimental infection with T. cruzi Colombian strain reduces myocarditis due to lower IFN-γ production and greater IL-10 production in situ and this pattern is not influenced by splenocyte general repertoire.http://dx.doi.org/10.1155/2018/3421897 |
| spellingShingle | Marcos Vinicius da Silva Vera Lúcia de Almeida Wendyson Duarte de Oliveira Natália Carasek Matos Cascudo Pollyana Guimarães de Oliveira Crislaine Aparecida da Silva Aline Cristina Souza da Silva Maria Luíza Gonçalves dos Reis Monteiro Rosana Rosa Miranda Correa Milton Adriano Pelli Oliveira Ruy de Sousa Lino-Júnior Mara Rúbia Nunes Celes Juliana Reis Machado Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi Mediators of Inflammation |
| title | Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi |
| title_full | Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi |
| title_fullStr | Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi |
| title_full_unstemmed | Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi |
| title_short | Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi |
| title_sort | upregulation of cardiac il 10 and downregulation of ifn γ in balb c il 4 in acute chagasic myocarditis due to colombian strain of trypanosoma cruzi |
| url | http://dx.doi.org/10.1155/2018/3421897 |
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