Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites
The Asteraceae are the largest plant family but among the least studied at the genome level. Our work investigated practical methods to reduce the influence of secondary metabolites – specifically, phenolic compounds – on the extraction of DNA from Silphium spp. This genus is in the Heliantheae trib...
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| Language: | English |
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Taylor & Francis Group
2022-03-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2021-0050 |
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| author | Dustin W Bailey Ziv Attia Stephan Reinert Brent S Hulke Nolan C Kane |
| author_facet | Dustin W Bailey Ziv Attia Stephan Reinert Brent S Hulke Nolan C Kane |
| author_sort | Dustin W Bailey |
| collection | DOAJ |
| description | The Asteraceae are the largest plant family but among the least studied at the genome level. Our work investigated practical methods to reduce the influence of secondary metabolites – specifically, phenolic compounds – on the extraction of DNA from Silphium spp. This genus is in the Heliantheae tribe of Asteraceae that also includes sunflower (Helianthus annuus). Three methods were attempted in Silphium, with varying condition and age of the leaf sample. A modified cetyl trimethylammonium bromide (CTAB) method on young leaves resulted in the best DNA yield, with sufficient sample purity. No perceptible difference was observed between fresh and lyophilized samples for any extraction method or leaf age. These results provide an excellent basis for DNA extraction of difficult plant samples. |
| format | Article |
| id | doaj-art-e2bec8df3c534fd682d2ffacac19db31 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-e2bec8df3c534fd682d2ffacac19db312025-08-20T02:25:34ZengTaylor & Francis GroupBioTechniques0736-62051940-98182022-03-01723858910.2144/btn-2021-0050Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolitesDustin W Bailey0Ziv Attia1Stephan Reinert2Brent S Hulke3Nolan C Kane41Ecology & Evolutionary Biology Department, University of Colorado, Boulder, CO 80309, USA1Ecology & Evolutionary Biology Department, University of Colorado, Boulder, CO 80309, USA1Ecology & Evolutionary Biology Department, University of Colorado, Boulder, CO 80309, USA3Sunflower and Plant Biology Research Unit, USDA-ARS Edward T. Schafer Agricultural Research Center, Fargo, ND 58102, USA1Ecology & Evolutionary Biology Department, University of Colorado, Boulder, CO 80309, USAThe Asteraceae are the largest plant family but among the least studied at the genome level. Our work investigated practical methods to reduce the influence of secondary metabolites – specifically, phenolic compounds – on the extraction of DNA from Silphium spp. This genus is in the Heliantheae tribe of Asteraceae that also includes sunflower (Helianthus annuus). Three methods were attempted in Silphium, with varying condition and age of the leaf sample. A modified cetyl trimethylammonium bromide (CTAB) method on young leaves resulted in the best DNA yield, with sufficient sample purity. No perceptible difference was observed between fresh and lyophilized samples for any extraction method or leaf age. These results provide an excellent basis for DNA extraction of difficult plant samples.https://www.future-science.com/doi/10.2144/btn-2021-0050CTABDNA extractionsecondary metabolitesSilphiumsodium metabisulfite |
| spellingShingle | Dustin W Bailey Ziv Attia Stephan Reinert Brent S Hulke Nolan C Kane Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites BioTechniques CTAB DNA extraction secondary metabolites Silphium sodium metabisulfite |
| title | Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites |
| title_full | Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites |
| title_fullStr | Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites |
| title_full_unstemmed | Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites |
| title_short | Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites |
| title_sort | effective strategies for isolating dna from members of asteraceae with high concentrations of secondary metabolites |
| topic | CTAB DNA extraction secondary metabolites Silphium sodium metabisulfite |
| url | https://www.future-science.com/doi/10.2144/btn-2021-0050 |
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