Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study
<b>Background/Objectives</b>: Periodontitis is a common oral disease marked by gingival inflammation and alveolar bone loss. This study evaluated the efficacy of chamomile tincture and lidocaine hydrochloride (CLH) gel in mitigating periodontal inflammation and bone loss and uncovered th...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/12/11/2629 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850267378938544128 |
|---|---|
| author | Jiahui Sun Huiyi Wang Junhong Xiao Qiudong Yang Heyu Liu Zhengkun Yang Yuqi Liu Xin Huang Liu Yang Li Ma Zhengguo Cao |
| author_facet | Jiahui Sun Huiyi Wang Junhong Xiao Qiudong Yang Heyu Liu Zhengkun Yang Yuqi Liu Xin Huang Liu Yang Li Ma Zhengguo Cao |
| author_sort | Jiahui Sun |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Periodontitis is a common oral disease marked by gingival inflammation and alveolar bone loss. This study evaluated the efficacy of chamomile tincture and lidocaine hydrochloride (CLH) gel in mitigating periodontal inflammation and bone loss and uncovered the molecular mechanisms involved, both in vitro and in vivo. <b>Methods</b>: A periodontitis model was induced in Sprague Dawley rats by ligating the mandibular first molars. Sixty rats were divided into four groups: control (C), periodontitis (PD), periodontitis + CLH gel once daily (G1), and periodontitis + CLH gel thrice daily (G3). Clinical, micro-computed tomography (micro-CT), biological, and histological evaluations were performed, focusing on osteoclastogenesis, osteogenesis, and inflammatory cytokine production. The effect of CLH gel on inflammatory responses in RAW264.7 cells was also assessed through co-culture assays under <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) infection, with RNA-sequencing, qPCR, and Western blot analyses to explore underlying mechanisms. <b>Results</b>: CLH gel significantly reduced gingival and systemic inflammation and mitigated bone loss by enhancing the bone volume to tissue volume ratio and trabecular thickness via the RANKL/OPG axis in rats. The G3 group showed marked reductions in osteoclasts and increases in osterix-positive cells compared to other groups. In vitro, CLH gel reduced the inflammatory phenotype of macrophages in the periodontitis microenvironment by modulating Type II interferon (IFN-γ) networks. <b>Conclusions</b>: CLH gel reduced inflammation and bone loss in rat periodontitis, promoting osteogenesis and inhibiting osteoclastogenesis. It also suppressed macrophage inflammation via Type II interferon networks under <i>P. gingivalis</i> stimulation. These findings suggest that CLH gel has potential as an adjunctive therapy for periodontitis. |
| format | Article |
| id | doaj-art-e2b6ea8bb006482e80d59a893095a3eb |
| institution | OA Journals |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj-art-e2b6ea8bb006482e80d59a893095a3eb2025-08-20T01:53:49ZengMDPI AGBiomedicines2227-90592024-11-011211262910.3390/biomedicines12112629Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical StudyJiahui Sun0Huiyi Wang1Junhong Xiao2Qiudong Yang3Heyu Liu4Zhengkun Yang5Yuqi Liu6Xin Huang7Liu Yang8Li Ma9Zhengguo Cao10State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China<b>Background/Objectives</b>: Periodontitis is a common oral disease marked by gingival inflammation and alveolar bone loss. This study evaluated the efficacy of chamomile tincture and lidocaine hydrochloride (CLH) gel in mitigating periodontal inflammation and bone loss and uncovered the molecular mechanisms involved, both in vitro and in vivo. <b>Methods</b>: A periodontitis model was induced in Sprague Dawley rats by ligating the mandibular first molars. Sixty rats were divided into four groups: control (C), periodontitis (PD), periodontitis + CLH gel once daily (G1), and periodontitis + CLH gel thrice daily (G3). Clinical, micro-computed tomography (micro-CT), biological, and histological evaluations were performed, focusing on osteoclastogenesis, osteogenesis, and inflammatory cytokine production. The effect of CLH gel on inflammatory responses in RAW264.7 cells was also assessed through co-culture assays under <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) infection, with RNA-sequencing, qPCR, and Western blot analyses to explore underlying mechanisms. <b>Results</b>: CLH gel significantly reduced gingival and systemic inflammation and mitigated bone loss by enhancing the bone volume to tissue volume ratio and trabecular thickness via the RANKL/OPG axis in rats. The G3 group showed marked reductions in osteoclasts and increases in osterix-positive cells compared to other groups. In vitro, CLH gel reduced the inflammatory phenotype of macrophages in the periodontitis microenvironment by modulating Type II interferon (IFN-γ) networks. <b>Conclusions</b>: CLH gel reduced inflammation and bone loss in rat periodontitis, promoting osteogenesis and inhibiting osteoclastogenesis. It also suppressed macrophage inflammation via Type II interferon networks under <i>P. gingivalis</i> stimulation. These findings suggest that CLH gel has potential as an adjunctive therapy for periodontitis.https://www.mdpi.com/2227-9059/12/11/2629periodontitischamomileinflammationType II interferon (IFN-γ) |
| spellingShingle | Jiahui Sun Huiyi Wang Junhong Xiao Qiudong Yang Heyu Liu Zhengkun Yang Yuqi Liu Xin Huang Liu Yang Li Ma Zhengguo Cao Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study Biomedicines periodontitis chamomile inflammation Type II interferon (IFN-γ) |
| title | Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study |
| title_full | Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study |
| title_fullStr | Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study |
| title_full_unstemmed | Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study |
| title_short | Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study |
| title_sort | chamomile tincture and lidocaine hydrochloride gel ameliorates periodontitis a preclinical study |
| topic | periodontitis chamomile inflammation Type II interferon (IFN-γ) |
| url | https://www.mdpi.com/2227-9059/12/11/2629 |
| work_keys_str_mv | AT jiahuisun chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT huiyiwang chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT junhongxiao chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT qiudongyang chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT heyuliu chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT zhengkunyang chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT yuqiliu chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT xinhuang chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT liuyang chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT lima chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy AT zhengguocao chamomiletinctureandlidocainehydrochloridegelamelioratesperiodontitisapreclinicalstudy |