Yeast Surface-Displayed H5N1 Avian Influenza Vaccines
Highly pathogenic H5N1 avian influenza viruses pose a pandemic threat to human health. A rapid vaccine production against fast outbreak is desired. We report, herein, a paradigm-shift influenza vaccine technology by presenting H5N1 hemagglutinin (HA) to the surface of yeast. We demonstrated, for the...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4131324 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850167232768770048 |
|---|---|
| author | Han Lei Sha Jin Erik Karlsson Stacey Schultz-Cherry Kaiming Ye |
| author_facet | Han Lei Sha Jin Erik Karlsson Stacey Schultz-Cherry Kaiming Ye |
| author_sort | Han Lei |
| collection | DOAJ |
| description | Highly pathogenic H5N1 avian influenza viruses pose a pandemic threat to human health. A rapid vaccine production against fast outbreak is desired. We report, herein, a paradigm-shift influenza vaccine technology by presenting H5N1 hemagglutinin (HA) to the surface of yeast. We demonstrated, for the first time, that the HA surface-presented yeast can be used as influenza vaccines to elicit both humoral and cell-mediated immunity in mice. The HI titer of antisera reached up to 128 in vaccinated mice. A high level of H5N1 HA-specific IgG1 and IgG2a antibody production was detected after boost immunization. Furthermore, we demonstrated that the yeast surface-displayed HA preserves its antigenic sites. It preferentially binds to both avian- and human-type receptors. In addition, the vaccine exhibited high cross-reactivity to both homologous and heterologous H5N1 viruses. A high level production of anti-HA antibodies was detected in the mice five months after vaccination. Finally, our animal experimental results indicated that the yeast vaccine offered complete protection of mice from lethal H5N1 virus challenge. No severe side effect of yeast vaccines was noted in animal studies. This new technology allows for rapid and large-scale production of influenza vaccines for prepandemic preparation. |
| format | Article |
| id | doaj-art-e2b59236dcab4bbdbcf445f783a30975 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e2b59236dcab4bbdbcf445f783a309752025-08-20T02:21:14ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/41313244131324Yeast Surface-Displayed H5N1 Avian Influenza VaccinesHan Lei0Sha Jin1Erik Karlsson2Stacey Schultz-Cherry3Kaiming Ye4Department of Biomedical Engineering, Watson School of Engineering and Applied Sciences, Binghamton University, State University of New York (SUNY), Binghamton, NY 13902, USADepartment of Biomedical Engineering, Watson School of Engineering and Applied Sciences, Binghamton University, State University of New York (SUNY), Binghamton, NY 13902, USADepartment of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Biomedical Engineering, Watson School of Engineering and Applied Sciences, Binghamton University, State University of New York (SUNY), Binghamton, NY 13902, USAHighly pathogenic H5N1 avian influenza viruses pose a pandemic threat to human health. A rapid vaccine production against fast outbreak is desired. We report, herein, a paradigm-shift influenza vaccine technology by presenting H5N1 hemagglutinin (HA) to the surface of yeast. We demonstrated, for the first time, that the HA surface-presented yeast can be used as influenza vaccines to elicit both humoral and cell-mediated immunity in mice. The HI titer of antisera reached up to 128 in vaccinated mice. A high level of H5N1 HA-specific IgG1 and IgG2a antibody production was detected after boost immunization. Furthermore, we demonstrated that the yeast surface-displayed HA preserves its antigenic sites. It preferentially binds to both avian- and human-type receptors. In addition, the vaccine exhibited high cross-reactivity to both homologous and heterologous H5N1 viruses. A high level production of anti-HA antibodies was detected in the mice five months after vaccination. Finally, our animal experimental results indicated that the yeast vaccine offered complete protection of mice from lethal H5N1 virus challenge. No severe side effect of yeast vaccines was noted in animal studies. This new technology allows for rapid and large-scale production of influenza vaccines for prepandemic preparation.http://dx.doi.org/10.1155/2016/4131324 |
| spellingShingle | Han Lei Sha Jin Erik Karlsson Stacey Schultz-Cherry Kaiming Ye Yeast Surface-Displayed H5N1 Avian Influenza Vaccines Journal of Immunology Research |
| title | Yeast Surface-Displayed H5N1 Avian Influenza Vaccines |
| title_full | Yeast Surface-Displayed H5N1 Avian Influenza Vaccines |
| title_fullStr | Yeast Surface-Displayed H5N1 Avian Influenza Vaccines |
| title_full_unstemmed | Yeast Surface-Displayed H5N1 Avian Influenza Vaccines |
| title_short | Yeast Surface-Displayed H5N1 Avian Influenza Vaccines |
| title_sort | yeast surface displayed h5n1 avian influenza vaccines |
| url | http://dx.doi.org/10.1155/2016/4131324 |
| work_keys_str_mv | AT hanlei yeastsurfacedisplayedh5n1avianinfluenzavaccines AT shajin yeastsurfacedisplayedh5n1avianinfluenzavaccines AT erikkarlsson yeastsurfacedisplayedh5n1avianinfluenzavaccines AT staceyschultzcherry yeastsurfacedisplayedh5n1avianinfluenzavaccines AT kaimingye yeastsurfacedisplayedh5n1avianinfluenzavaccines |