Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheat
Studies of the genetic base and polymorphism of bread wheat cultivars aimed at identifying alleles of genes associated with high baking and other economically valuable traits seem to be relevant, since bread wheat, along with all representatives of the Triticeae tribe, has a huge genetic potential f...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2023-09-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/3856 |
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| author | A. A. Galimova B. R. Kuluev |
| author_facet | A. A. Galimova B. R. Kuluev |
| author_sort | A. A. Galimova |
| collection | DOAJ |
| description | Studies of the genetic base and polymorphism of bread wheat cultivars aimed at identifying alleles of genes associated with high baking and other economically valuable traits seem to be relevant, since bread wheat, along with all representatives of the Triticeae tribe, has a huge genetic potential for creating cultivars with high technological and rheological properties of grain flour. The aim of this study was sequencing and analysis of the nucleotide sequences of the Glu-B1-1 gene, and analysis of the predicted amino acid sequences of its protein product in three cultivars of bread wheat. Thus, in the course of genotyping cultivars and lines of bread wheat for the Glu-B1-1 gene, in the cultivars ‘Avesta’, ‘Leningradka krupnozernaya’ and line C-75094, previously undescribed changes in the size of amplifiable regions of the Glu-B1-1 gene for high-molecular-weight glutenins were found. Comparative analysis of the nucleotide sequences of these genes with known sequences showed the presence of two deletions in ‘Avesta’ and C-75094 and the presence of seven single-nucleotide substitutions in ‘Leningradka krupnozernaya’. Alignment of the predicted Glu-B1 amino acid sequences of the studied accessions and the standard cultivar carrying the Glu-B1-a allele showed that deletions in the amino acid sequences of ‘Avesta’ and C-75094 accessions are localized in the central domain of the protein and affect the amount of tri-, hexa-, and nonapeptides, and in ‘Leningradka krupnozernaya’, a decrease in GQQ and PGQGQQ by one unit was revealed. In addition, substitutions of five amino acids were found in ‘Leningradka krupnozernaya’. Thus, we have found previously undescribed deletions and substitutions in the nucleotide sequences of the Glu-B1-1 gene for high-molecular-weight glutenins, which lead to changes in amino acid sequences in functionally important regions, namely, in the central domains of protein molecules. The identified mutations can be used for genotyping bread wheat cultivars. |
| format | Article |
| id | doaj-art-e2b52f78dd1648f69827954d94c6524f |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-e2b52f78dd1648f69827954d94c6524f2025-02-01T09:58:12ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592023-09-0127543343910.18699/VJGB-23-521369Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheatA. A. Galimova0B. R. Kuluev1Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences; Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences; Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Studies of the genetic base and polymorphism of bread wheat cultivars aimed at identifying alleles of genes associated with high baking and other economically valuable traits seem to be relevant, since bread wheat, along with all representatives of the Triticeae tribe, has a huge genetic potential for creating cultivars with high technological and rheological properties of grain flour. The aim of this study was sequencing and analysis of the nucleotide sequences of the Glu-B1-1 gene, and analysis of the predicted amino acid sequences of its protein product in three cultivars of bread wheat. Thus, in the course of genotyping cultivars and lines of bread wheat for the Glu-B1-1 gene, in the cultivars ‘Avesta’, ‘Leningradka krupnozernaya’ and line C-75094, previously undescribed changes in the size of amplifiable regions of the Glu-B1-1 gene for high-molecular-weight glutenins were found. Comparative analysis of the nucleotide sequences of these genes with known sequences showed the presence of two deletions in ‘Avesta’ and C-75094 and the presence of seven single-nucleotide substitutions in ‘Leningradka krupnozernaya’. Alignment of the predicted Glu-B1 amino acid sequences of the studied accessions and the standard cultivar carrying the Glu-B1-a allele showed that deletions in the amino acid sequences of ‘Avesta’ and C-75094 accessions are localized in the central domain of the protein and affect the amount of tri-, hexa-, and nonapeptides, and in ‘Leningradka krupnozernaya’, a decrease in GQQ and PGQGQQ by one unit was revealed. In addition, substitutions of five amino acids were found in ‘Leningradka krupnozernaya’. Thus, we have found previously undescribed deletions and substitutions in the nucleotide sequences of the Glu-B1-1 gene for high-molecular-weight glutenins, which lead to changes in amino acid sequences in functionally important regions, namely, in the central domains of protein molecules. The identified mutations can be used for genotyping bread wheat cultivars.https://vavilov.elpub.ru/jour/article/view/3856baking qualityhigh-molecular-weight glutenin subunits<i>glu-1</i> genesgenotyping |
| spellingShingle | A. A. Galimova B. R. Kuluev Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheat Вавиловский журнал генетики и селекции baking quality high-molecular-weight glutenin subunits <i>glu-1</i> genes genotyping |
| title | Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheat |
| title_full | Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheat |
| title_fullStr | Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheat |
| title_full_unstemmed | Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheat |
| title_short | Identification of new nucleotide sequences of the <i>Glu-B1-1</i> gene encoding x-type glutenins in bread wheat |
| title_sort | identification of new nucleotide sequences of the i glu b1 1 i gene encoding x type glutenins in bread wheat |
| topic | baking quality high-molecular-weight glutenin subunits <i>glu-1</i> genes genotyping |
| url | https://vavilov.elpub.ru/jour/article/view/3856 |
| work_keys_str_mv | AT aagalimova identificationofnewnucleotidesequencesoftheiglub11igeneencodingxtypegluteninsinbreadwheat AT brkuluev identificationofnewnucleotidesequencesoftheiglub11igeneencodingxtypegluteninsinbreadwheat |