Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding
Abstract Owing to the inherently gradual nature of coagulation, the body fails in covalently crosslinking to stabilize clots rapidly, even with the aid of topical hemostats, thus inducing hemostatic failure and potential rebleeding. Although recently developed adhesives confer sealing bleeding sites...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-58204-8 |
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| author | Ting Chen Chaonan Xiao Xianjun Chen Ziyi Yang Jingwei Zhao Bingkun Bao Qingmei Zeng Li Jiang Xinyi Huang Yi Yang Qiuning Lin Wei Gong Linyong Zhu |
| author_facet | Ting Chen Chaonan Xiao Xianjun Chen Ziyi Yang Jingwei Zhao Bingkun Bao Qingmei Zeng Li Jiang Xinyi Huang Yi Yang Qiuning Lin Wei Gong Linyong Zhu |
| author_sort | Ting Chen |
| collection | DOAJ |
| description | Abstract Owing to the inherently gradual nature of coagulation, the body fails in covalently crosslinking to stabilize clots rapidly, even with the aid of topical hemostats, thus inducing hemostatic failure and potential rebleeding. Although recently developed adhesives confer sealing bleeding sites independently of coagulation, interfacial blood hampers their adhesion and practical applications. Here, we report a covalently reactive hemostat based on blood-imbibing and -crosslinking microparticles. Once contacting blood, the microparticles automatically mix with blood via imbibition and covalently crosslink with blood proteins and the tissue matrix before natural coagulation operates, rapidly forming a fortified clot with enhanced mechanical strength and tissue adhesion. In contrast to commercial hemostats, the microparticles achieve rapid hemostasis (within 30 seconds) and less blood loss (approximately 35 mg and 1 g in the rat and coagulopathic pig models, respectively), while effectively preventing blood-pressure-elevation-induced rebleeding in a rabbit model. This work advances the development and clinical translation of hemostats for rapid hemostasis and rebleeding prevention. |
| format | Article |
| id | doaj-art-e2955fd1abed424ca8fe8ecc03d8794b |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e2955fd1abed424ca8fe8ecc03d8794b2025-08-20T02:17:52ZengNature PortfolioNature Communications2041-17232025-04-0116111610.1038/s41467-025-58204-8Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleedingTing Chen0Chaonan Xiao1Xianjun Chen2Ziyi Yang3Jingwei Zhao4Bingkun Bao5Qingmei Zeng6Li Jiang7Xinyi Huang8Yi Yang9Qiuning Lin10Wei Gong11Linyong Zhu12School of Biomedical Engineering, Shanghai Jiao Tong UniversitySchool of Biomedical Engineering, Shanghai Jiao Tong UniversityOptogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyDepartment of General Surgery, Xinhua Hospital, affiliated to Shanghai Jiao Tong University School of MedicineSchool of Biomedical Engineering, Shanghai Jiao Tong UniversitySchool of Biomedical Engineering, Shanghai Jiao Tong UniversitySchool of Biomedical Engineering, Shanghai Jiao Tong UniversitySchool of Biomedical Engineering, Shanghai Jiao Tong UniversitySchool of Biomedical Engineering, Shanghai Jiao Tong UniversityOptogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologySchool of Biomedical Engineering, Shanghai Jiao Tong UniversityDepartment of General Surgery, Xinhua Hospital, affiliated to Shanghai Jiao Tong University School of MedicineSchool of Biomedical Engineering, Shanghai Jiao Tong UniversityAbstract Owing to the inherently gradual nature of coagulation, the body fails in covalently crosslinking to stabilize clots rapidly, even with the aid of topical hemostats, thus inducing hemostatic failure and potential rebleeding. Although recently developed adhesives confer sealing bleeding sites independently of coagulation, interfacial blood hampers their adhesion and practical applications. Here, we report a covalently reactive hemostat based on blood-imbibing and -crosslinking microparticles. Once contacting blood, the microparticles automatically mix with blood via imbibition and covalently crosslink with blood proteins and the tissue matrix before natural coagulation operates, rapidly forming a fortified clot with enhanced mechanical strength and tissue adhesion. In contrast to commercial hemostats, the microparticles achieve rapid hemostasis (within 30 seconds) and less blood loss (approximately 35 mg and 1 g in the rat and coagulopathic pig models, respectively), while effectively preventing blood-pressure-elevation-induced rebleeding in a rabbit model. This work advances the development and clinical translation of hemostats for rapid hemostasis and rebleeding prevention.https://doi.org/10.1038/s41467-025-58204-8 |
| spellingShingle | Ting Chen Chaonan Xiao Xianjun Chen Ziyi Yang Jingwei Zhao Bingkun Bao Qingmei Zeng Li Jiang Xinyi Huang Yi Yang Qiuning Lin Wei Gong Linyong Zhu Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding Nature Communications |
| title | Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding |
| title_full | Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding |
| title_fullStr | Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding |
| title_full_unstemmed | Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding |
| title_short | Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding |
| title_sort | covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding |
| url | https://doi.org/10.1038/s41467-025-58204-8 |
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