Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection
Periportal fibrosis in schistosomiasis has been associated to the host immune response to parasite antigens. We evaluated the immune response in S. mansoni infected individuals with different degrees of periportal fibrosis. Cytokine and chemokines were measured in serum and in supernatants of PBMC c...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2012/394981 |
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| author | Robson Da Paixão De Souza Luciana Santos Cardoso Giuseppe Tittoni Varela Lopes Maria Cecília F. Almeida Ricardo Riccio Oliveira Leda Maria Alcântara Edgar M. Carvalho Maria Ilma Araujo |
| author_facet | Robson Da Paixão De Souza Luciana Santos Cardoso Giuseppe Tittoni Varela Lopes Maria Cecília F. Almeida Ricardo Riccio Oliveira Leda Maria Alcântara Edgar M. Carvalho Maria Ilma Araujo |
| author_sort | Robson Da Paixão De Souza |
| collection | DOAJ |
| description | Periportal fibrosis in schistosomiasis has been associated to the host immune response to parasite antigens. We evaluated the immune response in S. mansoni infected individuals with different degrees of periportal fibrosis. Cytokine and chemokines were measured in serum and in supernatants of PBMC cultures stimulated with the soluble adult worm (SWAP) or egg (SEA) antigens, using a sandwich ELISA. The levels of IL-5 in response to SEA were higher in individuals with moderate to severe fibrosis (310.9 pg/mL) compared to individuals without fibrosis (36.8 pg/mL; P=0.0418). There was also a higher production of TNF-α in cultures stimulated with SWAP in patients with insipient fibrosis (1446 pg/mL) compared to those without fibrosis (756.1 pg/mL; P=0.0319). The serum levels of IL-13 and MIP-1α were higher in subjects without fibrosis than in those with moderate to severe fibrosis. However a positive association between serum levels of IL-13, TNF-α, MIP-1α, and RANTES and S. mansoni parasite burden was found. From these data we conclude that IL-5 and TNF-α may participate in liver pathology in schistosomiasis. The positive association between IL-13, TNF-α, MIP-1α, and RANTES with parasite burden, however, might predict the development of liver pathology. |
| format | Article |
| id | doaj-art-e26fc906906a438d877f77da5258a4a3 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-e26fc906906a438d877f77da5258a4a32025-02-03T00:59:52ZengWileyJournal of Parasitology Research2090-00232090-00312012-01-01201210.1155/2012/394981394981Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni InfectionRobson Da Paixão De Souza0Luciana Santos Cardoso1Giuseppe Tittoni Varela Lopes2Maria Cecília F. Almeida3Ricardo Riccio Oliveira4Leda Maria Alcântara5Edgar M. Carvalho6Maria Ilma Araujo7Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, BrazilPeriportal fibrosis in schistosomiasis has been associated to the host immune response to parasite antigens. We evaluated the immune response in S. mansoni infected individuals with different degrees of periportal fibrosis. Cytokine and chemokines were measured in serum and in supernatants of PBMC cultures stimulated with the soluble adult worm (SWAP) or egg (SEA) antigens, using a sandwich ELISA. The levels of IL-5 in response to SEA were higher in individuals with moderate to severe fibrosis (310.9 pg/mL) compared to individuals without fibrosis (36.8 pg/mL; P=0.0418). There was also a higher production of TNF-α in cultures stimulated with SWAP in patients with insipient fibrosis (1446 pg/mL) compared to those without fibrosis (756.1 pg/mL; P=0.0319). The serum levels of IL-13 and MIP-1α were higher in subjects without fibrosis than in those with moderate to severe fibrosis. However a positive association between serum levels of IL-13, TNF-α, MIP-1α, and RANTES and S. mansoni parasite burden was found. From these data we conclude that IL-5 and TNF-α may participate in liver pathology in schistosomiasis. The positive association between IL-13, TNF-α, MIP-1α, and RANTES with parasite burden, however, might predict the development of liver pathology.http://dx.doi.org/10.1155/2012/394981 |
| spellingShingle | Robson Da Paixão De Souza Luciana Santos Cardoso Giuseppe Tittoni Varela Lopes Maria Cecília F. Almeida Ricardo Riccio Oliveira Leda Maria Alcântara Edgar M. Carvalho Maria Ilma Araujo Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection Journal of Parasitology Research |
| title | Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection |
| title_full | Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection |
| title_fullStr | Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection |
| title_full_unstemmed | Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection |
| title_short | Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection |
| title_sort | cytokine and chemokine profile in individuals with different degrees of periportal fibrosis due to schistosoma mansoni infection |
| url | http://dx.doi.org/10.1155/2012/394981 |
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