Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variations

Background: Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor and widely known to play an important role in regulating growth traits in bovine. Aim: This study aimed to evaluate the association of PLAG1 polymorphisms with body weight and measurement traits of Brahman cattl...

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Main Authors: Baharudin Trilaksono, Amira Fathin Rodhiyah, Yuli Yanti, Joko Riyanto, Hendra Kurniawan, Muhammad Imron, Delly Nista, Yumoko Ginto, Pita Sudrajad, Muhammad Cahyadi
Format: Article
Language:English
Published: Tripoli University 2024-12-01
Series:Open Veterinary Journal
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Online Access:http://www.ejmanager.com/fulltextpdf.php?mno=214897
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author Baharudin Trilaksono
Amira Fathin Rodhiyah
Yuli Yanti
Joko Riyanto
Hendra Kurniawan
Muhammad Imron
Delly Nista
Yumoko Ginto
Pita Sudrajad
Muhammad Cahyadi
author_facet Baharudin Trilaksono
Amira Fathin Rodhiyah
Yuli Yanti
Joko Riyanto
Hendra Kurniawan
Muhammad Imron
Delly Nista
Yumoko Ginto
Pita Sudrajad
Muhammad Cahyadi
author_sort Baharudin Trilaksono
collection DOAJ
description Background: Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor and widely known to play an important role in regulating growth traits in bovine. Aim: This study aimed to evaluate the association of PLAG1 polymorphisms with body weight and measurement traits of Brahman cattle. Methods: A total of 57 blood samples of Brahman cattle and their body weight and measurement traits have been collected. Moreover, the PCR-RFLP method was used to determine the genotypes of the three polymorphisms in PLAG1, namely g.48308C>T, g.32212 (19 bp indel), and g.45233 T>C. A mix model effect was performed to evaluate the association of PLAG1 with body weight and measurement traits, while Tukey HSD test was used to compare the means among genotypes. Results: Genotype variations were observed for the g.48308C>T SNP and 19 bp indel. In details, the CC and CT genotypes of g.48308C>T SNP were identified and all possible genotypes of 19 bp indel were also identified. On the other hand, g.45233T>C SNP showed no variation. The statistical analysis revealed that SNP g.48308C>T and 19 bp indel had a significant effect on body weight and chest girth (PT SNP and DD genotype of 19 bp indel of the PLAG1 were favorable for those two traits. Conclusion: Based on these results, the SNP g.48308C>T and 19 bp indel in the PLAG1 could be as candidate marker for body weight and chest girth in Brahman cattle population at the Center for Superior Animal Breeding and Forage (BPTU-HPT) Sembawa. [Open Vet J 2024; 14(12.000): 3248-3256]
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publishDate 2024-12-01
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spelling doaj-art-e2643a07a4fb47ff9c48c975a9662cef2025-08-20T03:09:13ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-12-0114123248325610.5455/OVJ.2024.v14.i12.10214897Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variationsBaharudin Trilaksono0Amira Fathin Rodhiyah1Yuli Yanti2Joko Riyanto3Hendra Kurniawan4Muhammad Imron5Delly Nista6Yumoko Ginto7Pita Sudrajad8Muhammad CahyadiDepartment of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU HPT) Sembawa, Ministry of Agriculture Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU HPT) Sembawa, Ministry of Agriculture Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU HPT) Sembawa, Ministry of Agriculture Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU HPT) Sembawa, Ministry of Agriculture Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN) Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, IndonesiaBackground: Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor and widely known to play an important role in regulating growth traits in bovine. Aim: This study aimed to evaluate the association of PLAG1 polymorphisms with body weight and measurement traits of Brahman cattle. Methods: A total of 57 blood samples of Brahman cattle and their body weight and measurement traits have been collected. Moreover, the PCR-RFLP method was used to determine the genotypes of the three polymorphisms in PLAG1, namely g.48308C>T, g.32212 (19 bp indel), and g.45233 T>C. A mix model effect was performed to evaluate the association of PLAG1 with body weight and measurement traits, while Tukey HSD test was used to compare the means among genotypes. Results: Genotype variations were observed for the g.48308C>T SNP and 19 bp indel. In details, the CC and CT genotypes of g.48308C>T SNP were identified and all possible genotypes of 19 bp indel were also identified. On the other hand, g.45233T>C SNP showed no variation. The statistical analysis revealed that SNP g.48308C>T and 19 bp indel had a significant effect on body weight and chest girth (PT SNP and DD genotype of 19 bp indel of the PLAG1 were favorable for those two traits. Conclusion: Based on these results, the SNP g.48308C>T and 19 bp indel in the PLAG1 could be as candidate marker for body weight and chest girth in Brahman cattle population at the Center for Superior Animal Breeding and Forage (BPTU-HPT) Sembawa. [Open Vet J 2024; 14(12.000): 3248-3256]http://www.ejmanager.com/fulltextpdf.php?mno=214897association studybody weightbrahman cattlechest girthplag1 variations
spellingShingle Baharudin Trilaksono
Amira Fathin Rodhiyah
Yuli Yanti
Joko Riyanto
Hendra Kurniawan
Muhammad Imron
Delly Nista
Yumoko Ginto
Pita Sudrajad
Muhammad Cahyadi
Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variations
Open Veterinary Journal
association study
body weight
brahman cattle
chest girth
plag1 variations
title Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variations
title_full Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variations
title_fullStr Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variations
title_full_unstemmed Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variations
title_short Body weight and measurement traits of Brahman cattle affected by pleomorphic adenoma gene 1 (PLAG1) variations
title_sort body weight and measurement traits of brahman cattle affected by pleomorphic adenoma gene 1 plag1 variations
topic association study
body weight
brahman cattle
chest girth
plag1 variations
url http://www.ejmanager.com/fulltextpdf.php?mno=214897
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