Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study

Background: There is currently no specific requirement for operator’s attires performing amniocentesis, especially for repetitive amniocenteses. We investigated the contamination status on the surface of operators with or without long-sleeved surgical gowns during repetitive amniocenteses to examine...

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Main Authors: Jinyu Wang, Ying Song, Lingling Sun, Xijing Liu, Fan Zhou, Qianying Zhao, Jing Wang, Xuemei Zhang, Mei Yang, Dan Xie, Ting Hu, Shanling Liu
Format: Article
Language:English
Published: IMR Press 2024-07-01
Series:Clinical and Experimental Obstetrics & Gynecology
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Online Access:https://www.imrpress.com/journal/CEOG/51/7/10.31083/j.ceog5107162
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author Jinyu Wang
Ying Song
Lingling Sun
Xijing Liu
Fan Zhou
Qianying Zhao
Jing Wang
Xuemei Zhang
Mei Yang
Dan Xie
Ting Hu
Shanling Liu
author_facet Jinyu Wang
Ying Song
Lingling Sun
Xijing Liu
Fan Zhou
Qianying Zhao
Jing Wang
Xuemei Zhang
Mei Yang
Dan Xie
Ting Hu
Shanling Liu
author_sort Jinyu Wang
collection DOAJ
description Background: There is currently no specific requirement for operator’s attires performing amniocentesis, especially for repetitive amniocenteses. We investigated the contamination status on the surface of operators with or without long-sleeved surgical gowns during repetitive amniocenteses to examine whether they are different. Methods: In a prospective cohort study, consecutive samples were collected from the surface of amniocentesis operators with short-sleeved scrubs (bare arms) or with long-sleeved surgical gowns on 4 sites (including the left and right proximal medial forearm, and the left and right ventral wrist). There were 7 time spots during each sampling period. Results: The highest colony count among all plates was 65 colony forming units (cfu)/plate. We observed 85.3% (382/448) and 65.1% (255/392) plates with 0 colony count from operators with bare arms and in surgical gowns, respectively. Moreover, the bacterial load of the group with surgical gowns had an increasing trend with time while the other group showed a contrary trend. Conclusions: All colony counts observed were within the hygiene specification of the National Health Commission of China. Instead of attires, other measures with high-quality evidence including surveillance of hand hygiene and control of indoor air bacterial load should be given more attention.
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spelling doaj-art-e263cd8c56f84feb8010ff8973fdbd102025-08-20T02:04:21ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632024-07-0151716210.31083/j.ceog5107162S0390-6663(24)02433-3Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort StudyJinyu Wang0Ying Song1Lingling Sun2Xijing Liu3Fan Zhou4Qianying Zhao5Jing Wang6Xuemei Zhang7Mei Yang8Dan Xie9Ting Hu10Shanling Liu11Department of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, ChinaBackground: There is currently no specific requirement for operator’s attires performing amniocentesis, especially for repetitive amniocenteses. We investigated the contamination status on the surface of operators with or without long-sleeved surgical gowns during repetitive amniocenteses to examine whether they are different. Methods: In a prospective cohort study, consecutive samples were collected from the surface of amniocentesis operators with short-sleeved scrubs (bare arms) or with long-sleeved surgical gowns on 4 sites (including the left and right proximal medial forearm, and the left and right ventral wrist). There were 7 time spots during each sampling period. Results: The highest colony count among all plates was 65 colony forming units (cfu)/plate. We observed 85.3% (382/448) and 65.1% (255/392) plates with 0 colony count from operators with bare arms and in surgical gowns, respectively. Moreover, the bacterial load of the group with surgical gowns had an increasing trend with time while the other group showed a contrary trend. Conclusions: All colony counts observed were within the hygiene specification of the National Health Commission of China. Instead of attires, other measures with high-quality evidence including surveillance of hand hygiene and control of indoor air bacterial load should be given more attention.https://www.imrpress.com/journal/CEOG/51/7/10.31083/j.ceog5107162amniocentesissurgical gownsurgical scrubsurgical site infectioncolony countoperating room
spellingShingle Jinyu Wang
Ying Song
Lingling Sun
Xijing Liu
Fan Zhou
Qianying Zhao
Jing Wang
Xuemei Zhang
Mei Yang
Dan Xie
Ting Hu
Shanling Liu
Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study
Clinical and Experimental Obstetrics & Gynecology
amniocentesis
surgical gown
surgical scrub
surgical site infection
colony count
operating room
title Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study
title_full Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study
title_fullStr Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study
title_full_unstemmed Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study
title_short Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study
title_sort bacterial load on the surface of amniocentesis operators with or without long sleeved surgical gowns a prospective cohort study
topic amniocentesis
surgical gown
surgical scrub
surgical site infection
colony count
operating room
url https://www.imrpress.com/journal/CEOG/51/7/10.31083/j.ceog5107162
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