Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease
Crohn’s Disease (CD) is a chronic inflammatory bowel disease affecting the gastrointestinal tract. The search continues for new markers for assessing the activity of CD. Among them, pro-inflammatory and anti-inflammatory cytokines appear promising. We performed the analysis of cytokine concentration...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/15/1/116 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588939871911936 |
|---|---|
| author | Justyna Lorenc-Góra Dariusz Waniczek Zenon P. Czuba Mariusz Kryj Zbigniew Lorenc Małgorzata Muc-Wierzgoń |
| author_facet | Justyna Lorenc-Góra Dariusz Waniczek Zenon P. Czuba Mariusz Kryj Zbigniew Lorenc Małgorzata Muc-Wierzgoń |
| author_sort | Justyna Lorenc-Góra |
| collection | DOAJ |
| description | Crohn’s Disease (CD) is a chronic inflammatory bowel disease affecting the gastrointestinal tract. The search continues for new markers for assessing the activity of CD. Among them, pro-inflammatory and anti-inflammatory cytokines appear promising. We performed the analysis of cytokine concentrations in blood serum using the Bio-Plex Multiplex system (Bio-Rad), and their correlations with radiological parameters were assessed by magnetic resonance enterography (MRE), and fecal calprotectin levels were measured quantitatively by ELISA and clinical evaluation according to the Crohn’s Disease Activity Index (CDAI). Our study found that measuring cytokine serum concentrations can be a valuable tool in the diagnosis and treatment of CD. Positive correlations were reported between contrast enhancement on DCE-MRE and the concentrations of PDGF-BB and RANTES. Also, a positive correlation was found between the delayed-phase of DCE and IL-10 concentration, a strong negative correlation between the delayed-phase of DCE and IL-12 concentration, and a strong positive correlation between the delayed-phase of DCE and RANTES concentrations. A strong positive correlation was also observed between the thickness of the intestinal wall on T2-weighted images and RANTES concentration. Therefore, concentrations of PDGF-BB, RANTES, IL-10 and IL-12 are promising markers of CD activity. The study also demonstrated significant correlations between the severity of disease activity assessed by the CDAI and the concentrations of IL-5, IL-8 and IL-9, as well as positive correlations between the levels of fecal calprotectin and the concentrations of IL-1RA and VEGF. Therefore, the levels of IL-5, IL-8, IL-9, VEGF and IL-1RA may be useful markers in the diagnosis and clinical assessment of disease activity. |
| format | Article |
| id | doaj-art-e252d399c4754e4b8a1a80cbdfce4d51 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-e252d399c4754e4b8a1a80cbdfce4d512025-01-24T13:25:15ZengMDPI AGBiomolecules2218-273X2025-01-0115111610.3390/biom15010116Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s DiseaseJustyna Lorenc-Góra0Dariusz Waniczek1Zenon P. Czuba2Mariusz Kryj3Zbigniew Lorenc4Małgorzata Muc-Wierzgoń5Department of Imaging Diagnostics, St Barbara Regional Specialist Hospital No 5, 41-214 Sosnowiec, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, PolandDepartment of Microbiology and Immunology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, PolandDepartment of General and Colorectal Surgery and Multiple Trauma, St Barbara Regional Specialist Hospital No 5, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Internal Diseases Propaedeutics and Emergency Medicine, Faculty of Public Health in Bytom, Medical University of Silesia in Katowice, Piekarska 18, 44-902 Bytom, PolandCrohn’s Disease (CD) is a chronic inflammatory bowel disease affecting the gastrointestinal tract. The search continues for new markers for assessing the activity of CD. Among them, pro-inflammatory and anti-inflammatory cytokines appear promising. We performed the analysis of cytokine concentrations in blood serum using the Bio-Plex Multiplex system (Bio-Rad), and their correlations with radiological parameters were assessed by magnetic resonance enterography (MRE), and fecal calprotectin levels were measured quantitatively by ELISA and clinical evaluation according to the Crohn’s Disease Activity Index (CDAI). Our study found that measuring cytokine serum concentrations can be a valuable tool in the diagnosis and treatment of CD. Positive correlations were reported between contrast enhancement on DCE-MRE and the concentrations of PDGF-BB and RANTES. Also, a positive correlation was found between the delayed-phase of DCE and IL-10 concentration, a strong negative correlation between the delayed-phase of DCE and IL-12 concentration, and a strong positive correlation between the delayed-phase of DCE and RANTES concentrations. A strong positive correlation was also observed between the thickness of the intestinal wall on T2-weighted images and RANTES concentration. Therefore, concentrations of PDGF-BB, RANTES, IL-10 and IL-12 are promising markers of CD activity. The study also demonstrated significant correlations between the severity of disease activity assessed by the CDAI and the concentrations of IL-5, IL-8 and IL-9, as well as positive correlations between the levels of fecal calprotectin and the concentrations of IL-1RA and VEGF. Therefore, the levels of IL-5, IL-8, IL-9, VEGF and IL-1RA may be useful markers in the diagnosis and clinical assessment of disease activity.https://www.mdpi.com/2218-273X/15/1/116Crohn’s Disease (CD)inflammatory markerscytokinescalprotectinmagnetic resonance enterography (MRE)CDAI |
| spellingShingle | Justyna Lorenc-Góra Dariusz Waniczek Zenon P. Czuba Mariusz Kryj Zbigniew Lorenc Małgorzata Muc-Wierzgoń Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease Biomolecules Crohn’s Disease (CD) inflammatory markers cytokines calprotectin magnetic resonance enterography (MRE) CDAI |
| title | Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease |
| title_full | Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease |
| title_fullStr | Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease |
| title_full_unstemmed | Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease |
| title_short | Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease |
| title_sort | assessment of the utility of selected inflammatory markers in correlation with magnetic resonance enterography mre findings in the diagnosis of crohn s disease |
| topic | Crohn’s Disease (CD) inflammatory markers cytokines calprotectin magnetic resonance enterography (MRE) CDAI |
| url | https://www.mdpi.com/2218-273X/15/1/116 |
| work_keys_str_mv | AT justynalorencgora assessmentoftheutilityofselectedinflammatorymarkersincorrelationwithmagneticresonanceenterographymrefindingsinthediagnosisofcrohnsdisease AT dariuszwaniczek assessmentoftheutilityofselectedinflammatorymarkersincorrelationwithmagneticresonanceenterographymrefindingsinthediagnosisofcrohnsdisease AT zenonpczuba assessmentoftheutilityofselectedinflammatorymarkersincorrelationwithmagneticresonanceenterographymrefindingsinthediagnosisofcrohnsdisease AT mariuszkryj assessmentoftheutilityofselectedinflammatorymarkersincorrelationwithmagneticresonanceenterographymrefindingsinthediagnosisofcrohnsdisease AT zbigniewlorenc assessmentoftheutilityofselectedinflammatorymarkersincorrelationwithmagneticresonanceenterographymrefindingsinthediagnosisofcrohnsdisease AT małgorzatamucwierzgon assessmentoftheutilityofselectedinflammatorymarkersincorrelationwithmagneticresonanceenterographymrefindingsinthediagnosisofcrohnsdisease |