Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in Morocco
Monitoring <i>Campylobacter</i> and antimicrobial resistance is critical, as its presence in the food chain, particularly in poultry, represents a serious threat to public health. However, despite its significant impact, this bacterium remains largely underestimated and under-surveilled...
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MDPI AG
2025-05-01
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| author | Zineb Soubai Nadia Ziyate Sami Darkaoui Rim Rais Siham Fellahi Benaissa Attarassi Nabila Auajjar |
| author_facet | Zineb Soubai Nadia Ziyate Sami Darkaoui Rim Rais Siham Fellahi Benaissa Attarassi Nabila Auajjar |
| author_sort | Zineb Soubai |
| collection | DOAJ |
| description | Monitoring <i>Campylobacter</i> and antimicrobial resistance is critical, as its presence in the food chain, particularly in poultry, represents a serious threat to public health. However, despite its significant impact, this bacterium remains largely underestimated and under-surveilled in many countries, including Morocco. The current study aims to understand the occurrence of <i>Campylobacter</i> spp. in different poultry production systems, highlighting the importance of continuously monitoring antimicrobial susceptibility in these bacteria. For this purpose, 300 poultry samples were collected from a slaughterhouse and 254 from traditional markets in the Rabat-Salé-Kénitra region. The research on <i>Campylobacter</i> was performed according to the standard ISO10272-1(2017), and the confirmation of <i>Campylobacter</i> species was carried out using MALDI-TOF mass spectrometry. Additionally, the disk diffusion method was employed to assess antimicrobial susceptibility, and conventional PCR was utilized to detect the presence of the <i>tet(O)</i> gene in tetracycline-resistant strains. Out of 554 samples collected, 159 (28.7%) tested positive for <i>Campylobacter</i>, of which 84% were identified as <i>Campylobacter coli</i> and 16% as <i>Campylobacter jejuni</i>. Moreover, the results revealed a notably higher detection of <i>Campylobacter</i> in traditional markets (41.33%) than in slaughterhouses (19.85%). Additionally, it was more prevalent in chicken samples (35.37%) than in turkey samples (22.02%). <i>Campylobacter</i> resistance to antimicrobials revealed a resistance rate of 99% to ciprofloxacin, 73% to ampicillin, 82% to tetracycline, 29% to erythromycin, and 0% to gentamicin, as well as to the association of amoxicillin and clavulanic acid. Among the strains with a phenotypic resistance profile to tetracycline, 80% carried the <i>tet(O)</i> gene. The results provide an overview of the current state of antibiotic resistance in <i>Campylobacter</i> in Morocco. They underline the urgent need for the prudent use of antimicrobials and may encourage further research into the development of new antimicrobial agents. They also highlight the importance of raising awareness of the risk of carcass contamination |
| format | Article |
| id | doaj-art-e251b2bd0adc47bb9f5f478c93b4e409 |
| institution | DOAJ |
| issn | 2674-1164 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Poultry |
| spelling | doaj-art-e251b2bd0adc47bb9f5f478c93b4e4092025-08-20T03:16:36ZengMDPI AGPoultry2674-11642025-05-01422310.3390/poultry4020023Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in MoroccoZineb Soubai0Nadia Ziyate1Sami Darkaoui2Rim Rais3Siham Fellahi4Benaissa Attarassi5Nabila Auajjar6Laboratory of Biology and Health, Faculty of Sciences of Kénitra, Ibn Tofail University, Kénitra 14000, MoroccoDivision of Pharmacy and Veterinary Inputs, National Office for Food Safety (ONSSA), Rabat 10050, MoroccoDivision of Pharmacy and Veterinary Inputs, National Office for Food Safety (ONSSA), Rabat 10050, MoroccoLaboratory of Human Pathologies Biology, Department of Biology, Faculty of Sciences, Mohammed V University in Rabat, Rabat 10106, MoroccoAvian Pathology Unit, Department of Pathology and Veterinary Public Health, Hassan II Agronomic and Veterinary Institute, Rabat 10000, MoroccoLaboratory of Biology and Health, Faculty of Sciences of Kénitra, Ibn Tofail University, Kénitra 14000, MoroccoLaboratory of Biology and Health, Faculty of Sciences of Kénitra, Ibn Tofail University, Kénitra 14000, MoroccoMonitoring <i>Campylobacter</i> and antimicrobial resistance is critical, as its presence in the food chain, particularly in poultry, represents a serious threat to public health. However, despite its significant impact, this bacterium remains largely underestimated and under-surveilled in many countries, including Morocco. The current study aims to understand the occurrence of <i>Campylobacter</i> spp. in different poultry production systems, highlighting the importance of continuously monitoring antimicrobial susceptibility in these bacteria. For this purpose, 300 poultry samples were collected from a slaughterhouse and 254 from traditional markets in the Rabat-Salé-Kénitra region. The research on <i>Campylobacter</i> was performed according to the standard ISO10272-1(2017), and the confirmation of <i>Campylobacter</i> species was carried out using MALDI-TOF mass spectrometry. Additionally, the disk diffusion method was employed to assess antimicrobial susceptibility, and conventional PCR was utilized to detect the presence of the <i>tet(O)</i> gene in tetracycline-resistant strains. Out of 554 samples collected, 159 (28.7%) tested positive for <i>Campylobacter</i>, of which 84% were identified as <i>Campylobacter coli</i> and 16% as <i>Campylobacter jejuni</i>. Moreover, the results revealed a notably higher detection of <i>Campylobacter</i> in traditional markets (41.33%) than in slaughterhouses (19.85%). Additionally, it was more prevalent in chicken samples (35.37%) than in turkey samples (22.02%). <i>Campylobacter</i> resistance to antimicrobials revealed a resistance rate of 99% to ciprofloxacin, 73% to ampicillin, 82% to tetracycline, 29% to erythromycin, and 0% to gentamicin, as well as to the association of amoxicillin and clavulanic acid. Among the strains with a phenotypic resistance profile to tetracycline, 80% carried the <i>tet(O)</i> gene. The results provide an overview of the current state of antibiotic resistance in <i>Campylobacter</i> in Morocco. They underline the urgent need for the prudent use of antimicrobials and may encourage further research into the development of new antimicrobial agents. They also highlight the importance of raising awareness of the risk of carcass contaminationhttps://www.mdpi.com/2674-1164/4/2/23poultry<i>Campylobacter</i> spp.antimicrobial resistanceMALDI-TOF MSslaughterhousetraditional market |
| spellingShingle | Zineb Soubai Nadia Ziyate Sami Darkaoui Rim Rais Siham Fellahi Benaissa Attarassi Nabila Auajjar Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in Morocco Poultry poultry <i>Campylobacter</i> spp. antimicrobial resistance MALDI-TOF MS slaughterhouse traditional market |
| title | Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in Morocco |
| title_full | Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in Morocco |
| title_fullStr | Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in Morocco |
| title_full_unstemmed | Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in Morocco |
| title_short | Antimicrobial Susceptibility Profiles of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Processed Chickens and Turkeys in Morocco |
| title_sort | antimicrobial susceptibility profiles of i campylobacter jejuni i and i campylobacter coli i isolated from processed chickens and turkeys in morocco |
| topic | poultry <i>Campylobacter</i> spp. antimicrobial resistance MALDI-TOF MS slaughterhouse traditional market |
| url | https://www.mdpi.com/2674-1164/4/2/23 |
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