GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES
Graphene-based materials have an opportunity for use in biomedicine, thanks to their properties. Nevertheless, due to its cytotoxic effects, the use of graphene-based drugs is problematic. However, the surface modification of graphene oxide (GO) nanoparticles with a polyethyleneglycol (PEG) is one w...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2021-10-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/2414 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849240762081869824 |
|---|---|
| author | S. V. Uzhviyuk M. S. Bochkova V. P. Timganova P. V. Khramtsov K. Yu. Shardina M. D. Kropaneva A. I. Nechaev M. B. Rayev S. A. Zamorina |
| author_facet | S. V. Uzhviyuk M. S. Bochkova V. P. Timganova P. V. Khramtsov K. Yu. Shardina M. D. Kropaneva A. I. Nechaev M. B. Rayev S. A. Zamorina |
| author_sort | S. V. Uzhviyuk |
| collection | DOAJ |
| description | Graphene-based materials have an opportunity for use in biomedicine, thanks to their properties. Nevertheless, due to its cytotoxic effects, the use of graphene-based drugs is problematic. However, the surface modification of graphene oxide (GO) nanoparticles with a polyethyleneglycol (PEG) is one way to reduce the harmful effects of graphene on cells. Applying nanoparticles implies their interaction with the immune system, which protects the body. Monocytes are innate immunity cells and the first line of defenсe of the human organism from microorganisms and other alien objects. One of the monocytes’ reactions to a stimulus of any nature is to produce reactive oxygen species (ROS). Published data shows an incomplete picture of modified graphene oxide nanoparticles’ effects on ROS formation by human monocytes. Thus, it was essential to evaluate the pegylated graphene oxide (GO-PEG and GO-8armedPEG) effect on ROS production by human monocytes, assessed by the luminol-dependent chemiluminescence (LCL). The objects of the study were CD14+-cells isolated from mononuclear cells of healthy donors. ROS production was stimulated by opsonized zymosan (OZ), spontaneous LCL was used as a control. PEG-modified (GO-PEG and GO-8armedPEG) GO nanoparticles with sizes of 100-200 nm (“small”) and 1-5 μm (“big”) with PEG covering ~ 20% were used at concentrations of 5 and 25 μg/ml. The study showed that small size nanoparticles at a low concentration of 5 μg/ml and big nanoparticles coated with 8-armed PEG at both concentrations have a significant suppressive effect on spontaneous ROS production. In the stimulated LCL reaction variant, it was found that small nanoparticles (25 μg/ml) also have a suppressive effect on ROS production, such as big-sized particles coated with linear PEG at the same concentration. Thus, we have established for the first time that graphene oxide nanoparticles functionalized with PEG are capable of inhibiting the ROS production by human monocytes, and therefore, we can speak of the antioxidant activity of GO-PEG. |
| format | Article |
| id | doaj-art-e2503c36b7d34cfc9f9d2ab7de92a4dc |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2021-10-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-e2503c36b7d34cfc9f9d2ab7de92a4dc2025-08-20T04:00:27ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2021-10-0123464765210.15789/1563-0625-GON-22071441GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTESS. V. Uzhviyuk0M. S. Bochkova1V. P. Timganova2P. V. Khramtsov3K. Yu. Shardina4M. D. Kropaneva5A. I. Nechaev6M. B. Rayev7S. A. Zamorina8Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State National Research UniversityInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of SciencesInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State National Research UniversityInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State National Research UniversityGraphene-based materials have an opportunity for use in biomedicine, thanks to their properties. Nevertheless, due to its cytotoxic effects, the use of graphene-based drugs is problematic. However, the surface modification of graphene oxide (GO) nanoparticles with a polyethyleneglycol (PEG) is one way to reduce the harmful effects of graphene on cells. Applying nanoparticles implies their interaction with the immune system, which protects the body. Monocytes are innate immunity cells and the first line of defenсe of the human organism from microorganisms and other alien objects. One of the monocytes’ reactions to a stimulus of any nature is to produce reactive oxygen species (ROS). Published data shows an incomplete picture of modified graphene oxide nanoparticles’ effects on ROS formation by human monocytes. Thus, it was essential to evaluate the pegylated graphene oxide (GO-PEG and GO-8armedPEG) effect on ROS production by human monocytes, assessed by the luminol-dependent chemiluminescence (LCL). The objects of the study were CD14+-cells isolated from mononuclear cells of healthy donors. ROS production was stimulated by opsonized zymosan (OZ), spontaneous LCL was used as a control. PEG-modified (GO-PEG and GO-8armedPEG) GO nanoparticles with sizes of 100-200 nm (“small”) and 1-5 μm (“big”) with PEG covering ~ 20% were used at concentrations of 5 and 25 μg/ml. The study showed that small size nanoparticles at a low concentration of 5 μg/ml and big nanoparticles coated with 8-armed PEG at both concentrations have a significant suppressive effect on spontaneous ROS production. In the stimulated LCL reaction variant, it was found that small nanoparticles (25 μg/ml) also have a suppressive effect on ROS production, such as big-sized particles coated with linear PEG at the same concentration. Thus, we have established for the first time that graphene oxide nanoparticles functionalized with PEG are capable of inhibiting the ROS production by human monocytes, and therefore, we can speak of the antioxidant activity of GO-PEG.https://www.mimmun.ru/mimmun/article/view/2414graphene oxidesurface modification of nanoparticlespolyethyleneglycolluminol-dependent chemiluminescencemonocytesreactive oxygen species |
| spellingShingle | S. V. Uzhviyuk M. S. Bochkova V. P. Timganova P. V. Khramtsov K. Yu. Shardina M. D. Kropaneva A. I. Nechaev M. B. Rayev S. A. Zamorina GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES Медицинская иммунология graphene oxide surface modification of nanoparticles polyethyleneglycol luminol-dependent chemiluminescence monocytes reactive oxygen species |
| title | GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES |
| title_full | GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES |
| title_fullStr | GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES |
| title_full_unstemmed | GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES |
| title_short | GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES |
| title_sort | graphene oxide nanoparticles in the regulation of the oxidative activity of human monocytes |
| topic | graphene oxide surface modification of nanoparticles polyethyleneglycol luminol-dependent chemiluminescence monocytes reactive oxygen species |
| url | https://www.mimmun.ru/mimmun/article/view/2414 |
| work_keys_str_mv | AT svuzhviyuk grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT msbochkova grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT vptimganova grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT pvkhramtsov grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT kyushardina grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT mdkropaneva grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT ainechaev grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT mbrayev grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes AT sazamorina grapheneoxidenanoparticlesintheregulationoftheoxidativeactivityofhumanmonocytes |