Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone Replacement
ABSTRACT: Objectives: The purpose of this work was to optimise printable polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) biomaterials with high percentages of β-TCP endowed with balanced mechanical characteristics to resemble human cancellous bone, presumably improving osteogenesis. Methods:...
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Elsevier
2024-12-01
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| Series: | International Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0020653924000674 |
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| author | Benedetta Ghezzi Biagio Matera Matteo Meglioli Francesca Rossi Donatella Duraccio Maria Giulia Faga Andrea Zappettini Guido Maria Macaluso Simone Lumetti |
| author_facet | Benedetta Ghezzi Biagio Matera Matteo Meglioli Francesca Rossi Donatella Duraccio Maria Giulia Faga Andrea Zappettini Guido Maria Macaluso Simone Lumetti |
| author_sort | Benedetta Ghezzi |
| collection | DOAJ |
| description | ABSTRACT: Objectives: The purpose of this work was to optimise printable polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) biomaterials with high percentages of β-TCP endowed with balanced mechanical characteristics to resemble human cancellous bone, presumably improving osteogenesis. Methods: PCL/β-TCP scaffolds were obtained from customised filaments for fused deposition modelling (FDM) 3D printing with increasing amounts of β-TCP. Samples mechanical features, surface topography and wettability were evaluated as well as cytocompatibility assays, cell adhesion and differentiation. Results: The parameters of the newly fabricated materila were optimal for PCL/β-TCP scaffold fabrication. Composite surfaces showed higher hydrophilicity compared with the controls, and their surface roughness sharply was higher, possibly due to the presence of β-TCP. The Young's modulus of the composites was significantly higher than that of pristine PCL, indicating that the intrinsic strength of β-TCP is beneficial for enhancing the elastic modulus of the composite biomaterials. All novel composite biomaterials supported greater cellular growth and stronger osteoblastic differentiation compared with the PCL control. Conclusions: This project highlights the possibility to fabricat, through an FDM solvent-free approach, PCL/β-TCP scaffolds of up to 70 % concentrations of β-TCP. overcoming the current lmit of 60 % stated in the literature. The combination of 3D printing and customised biomaterials allowed production of highly personalised scaffolds with optimal mechanical and biological features resembling the natural structure and the composition of bone. This underlines the promise of such structures for innovative approaches for bone and periodontal regeneration. |
| format | Article |
| id | doaj-art-e247fcd93f5944ecb925ebd720a4442e |
| institution | OA Journals |
| issn | 0020-6539 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Dental Journal |
| spelling | doaj-art-e247fcd93f5944ecb925ebd720a4442e2025-08-20T02:18:16ZengElsevierInternational Dental Journal0020-65392024-12-017461220123210.1016/j.identj.2024.02.013Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone ReplacementBenedetta Ghezzi0Biagio Matera1Matteo Meglioli2Francesca Rossi3Donatella Duraccio4Maria Giulia Faga5Andrea Zappettini6Guido Maria Macaluso7Simone Lumetti8Centro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy; Istituto dei Materiali per l'Elettronica ed il Magnetismo, Consiglio Nazionale delle Ricerche, Parma, ItalyCentro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, ItalyCentro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy; Corresponding author. Centro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, Via Gramsci 14, 43126 Parma, Italy.Istituto dei Materiali per l'Elettronica ed il Magnetismo, Consiglio Nazionale delle Ricerche, Parma, ItalyIstituto di Scienze e Tecnologie per l'Energia e la Mobilità Sostenibili, Consiglio Nazionale delle Ricerche, Torino, ItalyIstituto di Scienze e Tecnologie per l'Energia e la Mobilità Sostenibili, Consiglio Nazionale delle Ricerche, Torino, ItalyIstituto dei Materiali per l'Elettronica ed il Magnetismo, Consiglio Nazionale delle Ricerche, Parma, ItalyCentro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy; Istituto dei Materiali per l'Elettronica ed il Magnetismo, Consiglio Nazionale delle Ricerche, Parma, ItalyCentro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy; Istituto dei Materiali per l'Elettronica ed il Magnetismo, Consiglio Nazionale delle Ricerche, Parma, ItalyABSTRACT: Objectives: The purpose of this work was to optimise printable polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) biomaterials with high percentages of β-TCP endowed with balanced mechanical characteristics to resemble human cancellous bone, presumably improving osteogenesis. Methods: PCL/β-TCP scaffolds were obtained from customised filaments for fused deposition modelling (FDM) 3D printing with increasing amounts of β-TCP. Samples mechanical features, surface topography and wettability were evaluated as well as cytocompatibility assays, cell adhesion and differentiation. Results: The parameters of the newly fabricated materila were optimal for PCL/β-TCP scaffold fabrication. Composite surfaces showed higher hydrophilicity compared with the controls, and their surface roughness sharply was higher, possibly due to the presence of β-TCP. The Young's modulus of the composites was significantly higher than that of pristine PCL, indicating that the intrinsic strength of β-TCP is beneficial for enhancing the elastic modulus of the composite biomaterials. All novel composite biomaterials supported greater cellular growth and stronger osteoblastic differentiation compared with the PCL control. Conclusions: This project highlights the possibility to fabricat, through an FDM solvent-free approach, PCL/β-TCP scaffolds of up to 70 % concentrations of β-TCP. overcoming the current lmit of 60 % stated in the literature. The combination of 3D printing and customised biomaterials allowed production of highly personalised scaffolds with optimal mechanical and biological features resembling the natural structure and the composition of bone. This underlines the promise of such structures for innovative approaches for bone and periodontal regeneration.http://www.sciencedirect.com/science/article/pii/S0020653924000674Bone regenerationTissue engineering3D printingPCLβ-TCPSolvent-free |
| spellingShingle | Benedetta Ghezzi Biagio Matera Matteo Meglioli Francesca Rossi Donatella Duraccio Maria Giulia Faga Andrea Zappettini Guido Maria Macaluso Simone Lumetti Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone Replacement International Dental Journal Bone regeneration Tissue engineering 3D printing PCL β-TCP Solvent-free |
| title | Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone Replacement |
| title_full | Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone Replacement |
| title_fullStr | Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone Replacement |
| title_full_unstemmed | Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone Replacement |
| title_short | Composite PCL Scaffold With 70% β-TCP as Suitable Structure for Bone Replacement |
| title_sort | composite pcl scaffold with 70 β tcp as suitable structure for bone replacement |
| topic | Bone regeneration Tissue engineering 3D printing PCL β-TCP Solvent-free |
| url | http://www.sciencedirect.com/science/article/pii/S0020653924000674 |
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