Generation of Chimeric African Swine Fever Viruses Through <i>In Vitro</i> and <i>In Vivo</i> Intergenotypic Gene Complementation

Generation of Chimeric African Swine Fever Viruses Through <i>In Vitro</i> and <i>In Vivo</i> Intergenotypic Gene Complementation

<b>Background/Objectives</b>: African swine fever (ASF), a fatal febrile hemorrhagic disease in domestic pigs and Eurasian wild boars, is caused by ASF virus (ASFV). ASF continues to spread across the globe, causing a significant impact on the world’s pig industry. Recently, highly virul...

Full description

Saved in:
Bibliographic Details
Main Authors: Tomoya Kitamura, Kentaro Masujin, Mitsutaka Ikezawa, Aruna Ambagala, Takehiro Kokuho
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Vaccines
Subjects:
African swine fever virus
vaccines
chimerization
<i>in vitro</i>
<i>in vivo</i>
Online Access:https://www.mdpi.com/2076-393X/13/5/462
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<b>Background/Objectives</b>: African swine fever (ASF), a fatal febrile hemorrhagic disease in domestic pigs and Eurasian wild boars, is caused by ASF virus (ASFV). ASF continues to spread across the globe, causing a significant impact on the world’s pig industry. Recently, highly virulent chimeric ASFV (chASFV) strains with recombined genomes of the p72 genotype I and II viruses have been reported in China, Vietnam and Russia. <b>Methods</b>: In order to understand the propensity of ASFV genome for recombination, we attempted to experimentally generate chASFVs both in vitro and <i>in vivo</i> employing two distinct attenuated ASFV strains: OUR T88/3 (genotype I) and AQSΔB119L (genotype II). <b>Results</b>: When IPKM cells were co-infected with ASFV OUR T88/3 and AQSΔB119L strains, three genetically distinct chASFV emerged. When pigs were inoculated with the individual chASFV isolates, all pigs developed acute ASF. When four pigs were co-infected with ASFV OUR T88/3 and AQSΔB119L, all of them developed acute ASF and died or were euthanized. Three chASFV strains were successfully isolated from splenic homogenates from each pig. <b>Conclusions</b>: Our research indicates that genotype I and II chASFV with diverse genomes can be easily generated experimentally both in vitro and <i>in vivo</i>.
ISSN:2076-393X

Similar Items