Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury
Stroke is a cerebrovascular disease that results in decreased blood flow. Although Panax notoginseng (PN), a Chinese herbal medicine, has been proven to promote stroke recovery, its molecular mechanism remains unclear. In this study, middle cerebral artery occlusion (MCAO) was induced in rats with t...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/8873261 |
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| author | Lanqing Meng Qing Huang Xuebin Li Ping Liang Yueyong Li Xiaohua Huang Jingjie Zhao Qiuping Chen Rong Qiu Lan Li Chongdong Jian Hongfei Yao Jianmin Huang Xionglin Tang Zechen Wang Zhongheng Wei Jun Wu Liuzhi Wei Qiuju Wei Qianli Tang Lu Huang Jihua Wei Dinggui Lu Qunqiang Luo Kegong Xie Yang Ouyang Jian Chen Genliang Li Linxue Luo Linbo He Chenyi Zhuo Anding Xu Lingzhang Meng |
| author_facet | Lanqing Meng Qing Huang Xuebin Li Ping Liang Yueyong Li Xiaohua Huang Jingjie Zhao Qiuping Chen Rong Qiu Lan Li Chongdong Jian Hongfei Yao Jianmin Huang Xionglin Tang Zechen Wang Zhongheng Wei Jun Wu Liuzhi Wei Qiuju Wei Qianli Tang Lu Huang Jihua Wei Dinggui Lu Qunqiang Luo Kegong Xie Yang Ouyang Jian Chen Genliang Li Linxue Luo Linbo He Chenyi Zhuo Anding Xu Lingzhang Meng |
| author_sort | Lanqing Meng |
| collection | DOAJ |
| description | Stroke is a cerebrovascular disease that results in decreased blood flow. Although Panax notoginseng (PN), a Chinese herbal medicine, has been proven to promote stroke recovery, its molecular mechanism remains unclear. In this study, middle cerebral artery occlusion (MCAO) was induced in rats with thrombi generated by thread and subsequently treated with PN. After that, staining with 2,3,5-triphenyltetrazolium chloride was employed to evaluate the infarcted area, and electron microscopy was used to assess ultrastructural changes of the neurovascular unit. RNA-Seq was performed to determine the differential expressed genes (DEGs) which were then verified by qPCR. In total, 817 DEGs were identified to be related to the therapeutic effect of PN on stroke recovery. Further analysis by Gene Oncology analysis and Kyoto Encyclopedia of Genes and Genomes revealed that most of these genes were involved in the biological function of nerves and blood vessels through the regulation of neuroactive live receptor interactions of PI3K-Akt, Rap1, cAMP, and cGMP-PKG signaling, which included in the 18 pathways identified in our research, of which, 9 were reported firstly that related to PN’s neuroprotective effect. This research sheds light on the potential molecular mechanisms underlying the effects of PN on stroke recovery. |
| format | Article |
| id | doaj-art-e21966602c23496ab2043ec2ffb773af |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e21966602c23496ab2043ec2ffb773af2025-02-03T01:28:03ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/88732618873261Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion InjuryLanqing Meng0Qing Huang1Xuebin Li2Ping Liang3Yueyong Li4Xiaohua Huang5Jingjie Zhao6Qiuping Chen7Rong Qiu8Lan Li9Chongdong Jian10Hongfei Yao11Jianmin Huang12Xionglin Tang13Zechen Wang14Zhongheng Wei15Jun Wu16Liuzhi Wei17Qiuju Wei18Qianli Tang19Lu Huang20Jihua Wei21Dinggui Lu22Qunqiang Luo23Kegong Xie24Yang Ouyang25Jian Chen26Genliang Li27Linxue Luo28Linbo He29Chenyi Zhuo30Anding Xu31Lingzhang Meng32Jinan University, Guangzhou City, Guangdong Province, ChinaDepartment of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Neurology, The First People’s Hospital of Yulin, Yulin City, Guangxi Province, ChinaDepartment of Inverventional Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaJinan University, Guangzhou City, Guangdong Province, ChinaLife Science and Clinical Research Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Rehabilitation Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaGraduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaGraduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaGraduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaGraduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Inverventional Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaUrinary Surgery Department, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaCenter for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaCenter for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaCenter for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaGraduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaTrauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaTrauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaTrauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaTrauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaGraduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Biochemistry and Molecular Biology, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Gynaecology and Obstetrics, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaShangsi People's Hospital, Fangchenggang City, Guangxi Province, ChinaDepartment of Hepatological Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaJinan University, Guangzhou City, Guangdong Province, ChinaCenter for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaStroke is a cerebrovascular disease that results in decreased blood flow. Although Panax notoginseng (PN), a Chinese herbal medicine, has been proven to promote stroke recovery, its molecular mechanism remains unclear. In this study, middle cerebral artery occlusion (MCAO) was induced in rats with thrombi generated by thread and subsequently treated with PN. After that, staining with 2,3,5-triphenyltetrazolium chloride was employed to evaluate the infarcted area, and electron microscopy was used to assess ultrastructural changes of the neurovascular unit. RNA-Seq was performed to determine the differential expressed genes (DEGs) which were then verified by qPCR. In total, 817 DEGs were identified to be related to the therapeutic effect of PN on stroke recovery. Further analysis by Gene Oncology analysis and Kyoto Encyclopedia of Genes and Genomes revealed that most of these genes were involved in the biological function of nerves and blood vessels through the regulation of neuroactive live receptor interactions of PI3K-Akt, Rap1, cAMP, and cGMP-PKG signaling, which included in the 18 pathways identified in our research, of which, 9 were reported firstly that related to PN’s neuroprotective effect. This research sheds light on the potential molecular mechanisms underlying the effects of PN on stroke recovery.http://dx.doi.org/10.1155/2020/8873261 |
| spellingShingle | Lanqing Meng Qing Huang Xuebin Li Ping Liang Yueyong Li Xiaohua Huang Jingjie Zhao Qiuping Chen Rong Qiu Lan Li Chongdong Jian Hongfei Yao Jianmin Huang Xionglin Tang Zechen Wang Zhongheng Wei Jun Wu Liuzhi Wei Qiuju Wei Qianli Tang Lu Huang Jihua Wei Dinggui Lu Qunqiang Luo Kegong Xie Yang Ouyang Jian Chen Genliang Li Linxue Luo Linbo He Chenyi Zhuo Anding Xu Lingzhang Meng Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury Journal of Immunology Research |
| title | Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury |
| title_full | Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury |
| title_fullStr | Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury |
| title_full_unstemmed | Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury |
| title_short | Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury |
| title_sort | genes induced by panax notoginseng in a rodent model of ischemia reperfusion injury |
| url | http://dx.doi.org/10.1155/2020/8873261 |
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