PET Quantification of Cerebral Oxygen Metabolism in Small Animals
Understanding cerebral oxygen metabolism is of great importance in both clinical diagnosis and animal experiments because oxygen is a fundamental source of brain energy and supports brain functional activities. Since small animals such as rats are widely used to study various diseases including cere...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/159103 |
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| author | Takashi Temma Kazuhiro Koshino Tetsuaki Moriguchi Jun-ichiro Enmi Hidehiro Iida |
| author_facet | Takashi Temma Kazuhiro Koshino Tetsuaki Moriguchi Jun-ichiro Enmi Hidehiro Iida |
| author_sort | Takashi Temma |
| collection | DOAJ |
| description | Understanding cerebral oxygen metabolism is of great importance in both clinical diagnosis and animal experiments because oxygen is a fundamental source of brain energy and supports brain functional activities. Since small animals such as rats are widely used to study various diseases including cerebral ischemia, cerebrovascular diseases, and neurodegenerative diseases, the development of a noninvasive in vivo measurement method of cerebral oxygen metabolic parameters such as oxygen extraction fraction (OEF) and cerebral metabolic rate of oxygen (CMRO2) as well as cerebral blood flow (CBF) and cerebral blood volume (CBV) has been a priority. Although positron emission tomography (PET) with 15O labeled gas tracers has been recognized as a powerful way to evaluate cerebral oxygen metabolism in humans, this method could not be applied to rats due to technical problems and there were no reports of PET measurement of cerebral oxygen metabolism in rats until an 15O-O2 injection method was developed a decade ago. Herein, we introduce an intravenous administration method using two types of injectable 15O-O2 and an 15O-O2 gas inhalation method through an airway placed in the trachea, which enables oxygen metabolism measurements in rats. |
| format | Article |
| id | doaj-art-e216544aa56b48cf8b4b86222fa4e1f5 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e216544aa56b48cf8b4b86222fa4e1f52025-02-03T07:23:33ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/159103159103PET Quantification of Cerebral Oxygen Metabolism in Small AnimalsTakashi Temma0Kazuhiro Koshino1Tetsuaki Moriguchi2Jun-ichiro Enmi3Hidehiro Iida4Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, JapanDepartment of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, JapanDepartment of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, JapanDepartment of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, JapanDepartment of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, JapanUnderstanding cerebral oxygen metabolism is of great importance in both clinical diagnosis and animal experiments because oxygen is a fundamental source of brain energy and supports brain functional activities. Since small animals such as rats are widely used to study various diseases including cerebral ischemia, cerebrovascular diseases, and neurodegenerative diseases, the development of a noninvasive in vivo measurement method of cerebral oxygen metabolic parameters such as oxygen extraction fraction (OEF) and cerebral metabolic rate of oxygen (CMRO2) as well as cerebral blood flow (CBF) and cerebral blood volume (CBV) has been a priority. Although positron emission tomography (PET) with 15O labeled gas tracers has been recognized as a powerful way to evaluate cerebral oxygen metabolism in humans, this method could not be applied to rats due to technical problems and there were no reports of PET measurement of cerebral oxygen metabolism in rats until an 15O-O2 injection method was developed a decade ago. Herein, we introduce an intravenous administration method using two types of injectable 15O-O2 and an 15O-O2 gas inhalation method through an airway placed in the trachea, which enables oxygen metabolism measurements in rats.http://dx.doi.org/10.1155/2014/159103 |
| spellingShingle | Takashi Temma Kazuhiro Koshino Tetsuaki Moriguchi Jun-ichiro Enmi Hidehiro Iida PET Quantification of Cerebral Oxygen Metabolism in Small Animals The Scientific World Journal |
| title | PET Quantification of Cerebral Oxygen Metabolism in Small Animals |
| title_full | PET Quantification of Cerebral Oxygen Metabolism in Small Animals |
| title_fullStr | PET Quantification of Cerebral Oxygen Metabolism in Small Animals |
| title_full_unstemmed | PET Quantification of Cerebral Oxygen Metabolism in Small Animals |
| title_short | PET Quantification of Cerebral Oxygen Metabolism in Small Animals |
| title_sort | pet quantification of cerebral oxygen metabolism in small animals |
| url | http://dx.doi.org/10.1155/2014/159103 |
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