A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of Glucose
A colorimetric/fluorescent dual-sensing assay for the detection of glucose was established based on the peroxidase-like activity of fluorescent carbon quantum dots derived from corncob. In the system, glucose oxidase was added to specifically catalyze the oxidation of glucose to produce H2O2, which...
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China Food Publishing Company
2024-12-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-026.pdf |
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| author | GUAN Huanan, ZHANG Mingyi, WANG Dandan, ZHANG Zhuzehui |
| author_facet | GUAN Huanan, ZHANG Mingyi, WANG Dandan, ZHANG Zhuzehui |
| author_sort | GUAN Huanan, ZHANG Mingyi, WANG Dandan, ZHANG Zhuzehui |
| collection | DOAJ |
| description | A colorimetric/fluorescent dual-sensing assay for the detection of glucose was established based on the peroxidase-like activity of fluorescent carbon quantum dots derived from corncob. In the system, glucose oxidase was added to specifically catalyze the oxidation of glucose to produce H2O2, which was then reacted with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) under the catalysis of carbon quantum dots. Under optimized conditions, good linearity was observed between the absorbance (y) of the reaction system and glucose concentration (x) in the range of 0.001–0.5 mmol/L, which was fitted as follows: y = 10.907x + 0.659 5, R2 = 0.993 8, with a detection limit of 1.305 5 μmol/L. Moreover, good linearity was observed between the difference in fluorescence intensity (y) and glucose concentration (x) in the range of 0.001–0.1 mmol/L, which was fitted as follows: y = 9 172x + 4 438, R2 = 0.994 4, with a detection limit of 9.249 7 nmol/L. The method was applied to the detection of glucose in four real samples, with good spiked recovery rates and precision, which indicated that corncob fluorescent carbon quantum dots have the potential for application as an enzyme mimic in the detection of glucose. |
| format | Article |
| id | doaj-art-e212b02eb94f484cbee2e9a171ed4828 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-e212b02eb94f484cbee2e9a171ed48282025-02-05T09:08:01ZengChina Food Publishing CompanyShipin Kexue1002-66302024-12-01452423224110.7506/spkx1002-6630-20240428-262A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of GlucoseGUAN Huanan, ZHANG Mingyi, WANG Dandan, ZHANG Zhuzehui0(1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China; 2. School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212100, China)A colorimetric/fluorescent dual-sensing assay for the detection of glucose was established based on the peroxidase-like activity of fluorescent carbon quantum dots derived from corncob. In the system, glucose oxidase was added to specifically catalyze the oxidation of glucose to produce H2O2, which was then reacted with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) under the catalysis of carbon quantum dots. Under optimized conditions, good linearity was observed between the absorbance (y) of the reaction system and glucose concentration (x) in the range of 0.001–0.5 mmol/L, which was fitted as follows: y = 10.907x + 0.659 5, R2 = 0.993 8, with a detection limit of 1.305 5 μmol/L. Moreover, good linearity was observed between the difference in fluorescence intensity (y) and glucose concentration (x) in the range of 0.001–0.1 mmol/L, which was fitted as follows: y = 9 172x + 4 438, R2 = 0.994 4, with a detection limit of 9.249 7 nmol/L. The method was applied to the detection of glucose in four real samples, with good spiked recovery rates and precision, which indicated that corncob fluorescent carbon quantum dots have the potential for application as an enzyme mimic in the detection of glucose.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-026.pdfcarbon quantum dots; peroxidase mimic; colorimetric sensors; fluorescent sensors; glucose; food analysis |
| spellingShingle | GUAN Huanan, ZHANG Mingyi, WANG Dandan, ZHANG Zhuzehui A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of Glucose Shipin Kexue carbon quantum dots; peroxidase mimic; colorimetric sensors; fluorescent sensors; glucose; food analysis |
| title | A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of Glucose |
| title_full | A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of Glucose |
| title_fullStr | A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of Glucose |
| title_full_unstemmed | A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of Glucose |
| title_short | A Colorimetric/Fluorescent Dual Sensing System Based on the Enzyme-like Activity of Fluorescent Carbon Quantum Dots for Detection of Glucose |
| title_sort | colorimetric fluorescent dual sensing system based on the enzyme like activity of fluorescent carbon quantum dots for detection of glucose |
| topic | carbon quantum dots; peroxidase mimic; colorimetric sensors; fluorescent sensors; glucose; food analysis |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-026.pdf |
| work_keys_str_mv | AT guanhuananzhangmingyiwangdandanzhangzhuzehui acolorimetricfluorescentdualsensingsystembasedontheenzymelikeactivityoffluorescentcarbonquantumdotsfordetectionofglucose AT guanhuananzhangmingyiwangdandanzhangzhuzehui colorimetricfluorescentdualsensingsystembasedontheenzymelikeactivityoffluorescentcarbonquantumdotsfordetectionofglucose |