Utility and usability of a dengue NS1 rapid diagnostic as a self-test
Abstract Background Early detection of dengue fever is pivotal to help differentiate against other febrile illnesses, especially in the dengue-endemic regions. Currently, febrile patients would have to go to the healthcare facility to get tested for dengue. A diagnostic approach that empowers febril...
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| Language: | English |
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BMC
2025-02-01
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| Series: | Tropical Medicine and Health |
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| Online Access: | https://doi.org/10.1186/s41182-025-00705-9 |
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| author | Nurfatihah Zulkifli Naim Che-Kamaruddin Atiqah Hazan Tan Kim-Kee Sivalingam Rajagopal Sazaly AbuBakar |
| author_facet | Nurfatihah Zulkifli Naim Che-Kamaruddin Atiqah Hazan Tan Kim-Kee Sivalingam Rajagopal Sazaly AbuBakar |
| author_sort | Nurfatihah Zulkifli |
| collection | DOAJ |
| description | Abstract Background Early detection of dengue fever is pivotal to help differentiate against other febrile illnesses, especially in the dengue-endemic regions. Currently, febrile patients would have to go to the healthcare facility to get tested for dengue. A diagnostic approach that empowers febrile persons to perform their own tests is needed. Here, we evaluated the utility and the usability of the dengue NS1 rapid diagnostic test and whether it could be accepted as a home self-test. A lateral flow immunochromatography test (ICT) using DENV NS1 was converted to a possible self-test kit by providing the kit with a test device, an alcohol swab, a lancet, a disposable dropper, an assay buffer, and a test manual. Methods Twenty volunteers were recruited for this study. The participants performed the self-test under the observation of trained observers who recorded if any procedural error was committed. The results of each test were interpreted by the participants using a given interpretation table. Results Ninety-five percent (95%) of the study participants found the instruction manual was easy to follow and 70% felt the test kit was easy to use. Additionally, 80% of the participants successfully obtained the expected results. A majority (80%) would consider using the test kit if needed and would recommend it to family members and friends. Seventy percent (70%) of the participants, most of whom with monthly income of less than USD417, were willing to pay less than USD2 for the test kit. Conclusion Findings from the study suggested that a self-test diagnostic for dengue fever is highly acceptable and, hence, could be a viable approach for the early detection of the infection. Trial registration MRECID.NO: 2022628-11345. |
| format | Article |
| id | doaj-art-e204e64647a84b9e97a1288b2d63dc4e |
| institution | OA Journals |
| issn | 1349-4147 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Tropical Medicine and Health |
| spelling | doaj-art-e204e64647a84b9e97a1288b2d63dc4e2025-08-20T02:01:40ZengBMCTropical Medicine and Health1349-41472025-02-015311910.1186/s41182-025-00705-9Utility and usability of a dengue NS1 rapid diagnostic as a self-testNurfatihah Zulkifli0Naim Che-Kamaruddin1Atiqah Hazan2Tan Kim-Kee3Sivalingam Rajagopal4Sazaly AbuBakar5Tropical Infectious Diseases Research and Education Centre (TIDREC), Level 2, High Impact Research (HIR) Building, Universiti MalayaTropical Infectious Diseases Research and Education Centre (TIDREC), Level 2, High Impact Research (HIR) Building, Universiti MalayaTropical Infectious Diseases Research and Education Centre (TIDREC), Level 2, High Impact Research (HIR) Building, Universiti MalayaTropical Infectious Diseases Research and Education Centre (TIDREC), Level 2, High Impact Research (HIR) Building, Universiti MalayaAccobiotech Sdn. Bhd. Industrial ParkTropical Infectious Diseases Research and Education Centre (TIDREC), Level 2, High Impact Research (HIR) Building, Universiti MalayaAbstract Background Early detection of dengue fever is pivotal to help differentiate against other febrile illnesses, especially in the dengue-endemic regions. Currently, febrile patients would have to go to the healthcare facility to get tested for dengue. A diagnostic approach that empowers febrile persons to perform their own tests is needed. Here, we evaluated the utility and the usability of the dengue NS1 rapid diagnostic test and whether it could be accepted as a home self-test. A lateral flow immunochromatography test (ICT) using DENV NS1 was converted to a possible self-test kit by providing the kit with a test device, an alcohol swab, a lancet, a disposable dropper, an assay buffer, and a test manual. Methods Twenty volunteers were recruited for this study. The participants performed the self-test under the observation of trained observers who recorded if any procedural error was committed. The results of each test were interpreted by the participants using a given interpretation table. Results Ninety-five percent (95%) of the study participants found the instruction manual was easy to follow and 70% felt the test kit was easy to use. Additionally, 80% of the participants successfully obtained the expected results. A majority (80%) would consider using the test kit if needed and would recommend it to family members and friends. Seventy percent (70%) of the participants, most of whom with monthly income of less than USD417, were willing to pay less than USD2 for the test kit. Conclusion Findings from the study suggested that a self-test diagnostic for dengue fever is highly acceptable and, hence, could be a viable approach for the early detection of the infection. Trial registration MRECID.NO: 2022628-11345.https://doi.org/10.1186/s41182-025-00705-9Infectious diseasesArbovirusesDengue virusNS1Self-testUtility |
| spellingShingle | Nurfatihah Zulkifli Naim Che-Kamaruddin Atiqah Hazan Tan Kim-Kee Sivalingam Rajagopal Sazaly AbuBakar Utility and usability of a dengue NS1 rapid diagnostic as a self-test Tropical Medicine and Health Infectious diseases Arboviruses Dengue virus NS1 Self-test Utility |
| title | Utility and usability of a dengue NS1 rapid diagnostic as a self-test |
| title_full | Utility and usability of a dengue NS1 rapid diagnostic as a self-test |
| title_fullStr | Utility and usability of a dengue NS1 rapid diagnostic as a self-test |
| title_full_unstemmed | Utility and usability of a dengue NS1 rapid diagnostic as a self-test |
| title_short | Utility and usability of a dengue NS1 rapid diagnostic as a self-test |
| title_sort | utility and usability of a dengue ns1 rapid diagnostic as a self test |
| topic | Infectious diseases Arboviruses Dengue virus NS1 Self-test Utility |
| url | https://doi.org/10.1186/s41182-025-00705-9 |
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