LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives
Movements of plant pathogenic microorganisms in uncontaminated areas occur today at an alarming rate, driven mainly by global trade and climate change. These invaders can trigger new disease outbreaks able to impact the biodiversity and economies of vast territories and affect a variety of ecosystem...
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MDPI AG
2024-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/14/12/1549 |
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| author | Chiara Aglietti Alessandra Benigno Santa Olga Cacciola Salvatore Moricca |
| author_facet | Chiara Aglietti Alessandra Benigno Santa Olga Cacciola Salvatore Moricca |
| author_sort | Chiara Aglietti |
| collection | DOAJ |
| description | Movements of plant pathogenic microorganisms in uncontaminated areas occur today at an alarming rate, driven mainly by global trade and climate change. These invaders can trigger new disease outbreaks able to impact the biodiversity and economies of vast territories and affect a variety of ecosystem services. National and supranational regulatory deficiencies, such as inadequate quarantine measures and ineffective early pathogen detection at ports of entry, exacerbate the issue. Thus, there is an urgent need for accurate and rapid diagnostic tools to intercept invasive and nonindigenous plant pathogens. The LAMP (Loop-mediated isothermal AMPlification) technique is a robust, flexible tool representing a significant advance in point-of-care (POC) diagnostics. Its user-friendliness and sensitivity offer a breakthrough in phytosanitary checks at points of entry (harbors and airports), for disease and pest surveillance at vulnerable sites (e.g., nurseries and wood-processing and storage facilities), and for territorial monitoring of new disease outbreaks. This review highlights the strengths and weaknesses of LAMP, emphasizing its potential to revolutionize modern plant disease diagnostics. |
| format | Article |
| id | doaj-art-e1f539bdb69c4bfcb86ca2fcd0195927 |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-e1f539bdb69c4bfcb86ca2fcd01959272025-08-20T02:57:02ZengMDPI AGLife2075-17292024-11-011412154910.3390/life14121549LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future PerspectivesChiara Aglietti0Alessandra Benigno1Santa Olga Cacciola2Salvatore Moricca3Department of Agricultural, Food, Environmental and Forestry Science and Technology (DAGRI), Plant Pathology and Entomology Section, University of Florence, Piazzale delle Cascine 28, 50144 Florence, ItalyDepartment of Agricultural, Food, Environmental and Forestry Science and Technology (DAGRI), Plant Pathology and Entomology Section, University of Florence, Piazzale delle Cascine 28, 50144 Florence, ItalyDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyDepartment of Agricultural, Food, Environmental and Forestry Science and Technology (DAGRI), Plant Pathology and Entomology Section, University of Florence, Piazzale delle Cascine 28, 50144 Florence, ItalyMovements of plant pathogenic microorganisms in uncontaminated areas occur today at an alarming rate, driven mainly by global trade and climate change. These invaders can trigger new disease outbreaks able to impact the biodiversity and economies of vast territories and affect a variety of ecosystem services. National and supranational regulatory deficiencies, such as inadequate quarantine measures and ineffective early pathogen detection at ports of entry, exacerbate the issue. Thus, there is an urgent need for accurate and rapid diagnostic tools to intercept invasive and nonindigenous plant pathogens. The LAMP (Loop-mediated isothermal AMPlification) technique is a robust, flexible tool representing a significant advance in point-of-care (POC) diagnostics. Its user-friendliness and sensitivity offer a breakthrough in phytosanitary checks at points of entry (harbors and airports), for disease and pest surveillance at vulnerable sites (e.g., nurseries and wood-processing and storage facilities), and for territorial monitoring of new disease outbreaks. This review highlights the strengths and weaknesses of LAMP, emphasizing its potential to revolutionize modern plant disease diagnostics.https://www.mdpi.com/2075-1729/14/12/1549loop-mediated isothermal amplificationphytosanitary checkspathogen detectionglobal tradeclimate changeinvasive species |
| spellingShingle | Chiara Aglietti Alessandra Benigno Santa Olga Cacciola Salvatore Moricca LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives Life loop-mediated isothermal amplification phytosanitary checks pathogen detection global trade climate change invasive species |
| title | LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives |
| title_full | LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives |
| title_fullStr | LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives |
| title_full_unstemmed | LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives |
| title_short | LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives |
| title_sort | lamp reaction in plant disease surveillance applications challenges and future perspectives |
| topic | loop-mediated isothermal amplification phytosanitary checks pathogen detection global trade climate change invasive species |
| url | https://www.mdpi.com/2075-1729/14/12/1549 |
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