Molecular investigation of Hepatozoon canis in dogs in Mosul city
This study is the first molecular detection of Hepatozoon canis in dogs in Mosul city. One hundred twenty blood samples were collected from stray and household dogs of different ages, sexes, breeds, and breeding, suffering from emaciation, anemia, paleness in mucous membranes and the presence of tic...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_186155_730dc4a7888bd4b86224a31e76a20396.pdf |
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| author | Zeena D. Zaki Wasan A. Alobaidii |
| author_facet | Zeena D. Zaki Wasan A. Alobaidii |
| author_sort | Zeena D. Zaki |
| collection | DOAJ |
| description | This study is the first molecular detection of Hepatozoon canis in dogs in Mosul city. One hundred twenty blood samples were collected from stray and household dogs of different ages, sexes, breeds, and breeding, suffering from emaciation, anemia, paleness in mucous membranes and the presence of ticks in the ear. The DNA was extracted using a commercial kit (Qiagen, Germany). Polymerase Chain Reaction was used to detect the parasite targeting a partial region of 18S rRNA gene. The polymerase chain reaction produced positive results, as bands in electrophoresis at 737 bp. The total positive sample was 33 dogs out of 120 dogs (27.5%) the relationship between the infection with the parasite and the age of animals recorded a high prevalence of infection in dogs less than 6 months while the lowest prevalence of infection in animals more than 2 years old, The male dogs showed a high prevalence of infection when compared with female dog, The large breeds dogs recorded high prevalence of infection than small breeds dogs, The outdoor dogs showed a high prevalence of infection Animals which suffer from paleness in mucous membrane showed high prevalence, while healthy animals recorded lowest prevalence of infection, gene sequence showed there were only three isolates out of the total samples sent for genetic sequencing. |
| format | Article |
| id | doaj-art-e1c138700dff44abad49d0b1503fa270 |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-e1c138700dff44abad49d0b1503fa2702025-02-04T13:11:43ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-01-0139112913410.33899/ijvs.2024.151603.3768186155Molecular investigation of Hepatozoon canis in dogs in Mosul cityZeena D. Zaki0Wasan A. Alobaidii1Department of Biology, College of Education for Pure Science University of Mosul, Mosul, IraqDepartment of Microbiology, College of Veterinary Medicine, Mosul of University, Mosul, IraqThis study is the first molecular detection of Hepatozoon canis in dogs in Mosul city. One hundred twenty blood samples were collected from stray and household dogs of different ages, sexes, breeds, and breeding, suffering from emaciation, anemia, paleness in mucous membranes and the presence of ticks in the ear. The DNA was extracted using a commercial kit (Qiagen, Germany). Polymerase Chain Reaction was used to detect the parasite targeting a partial region of 18S rRNA gene. The polymerase chain reaction produced positive results, as bands in electrophoresis at 737 bp. The total positive sample was 33 dogs out of 120 dogs (27.5%) the relationship between the infection with the parasite and the age of animals recorded a high prevalence of infection in dogs less than 6 months while the lowest prevalence of infection in animals more than 2 years old, The male dogs showed a high prevalence of infection when compared with female dog, The large breeds dogs recorded high prevalence of infection than small breeds dogs, The outdoor dogs showed a high prevalence of infection Animals which suffer from paleness in mucous membrane showed high prevalence, while healthy animals recorded lowest prevalence of infection, gene sequence showed there were only three isolates out of the total samples sent for genetic sequencing.https://www.vetmedmosul.com/article_186155_730dc4a7888bd4b86224a31e76a20396.pdfhepatzoon canispcrdogsmosul |
| spellingShingle | Zeena D. Zaki Wasan A. Alobaidii Molecular investigation of Hepatozoon canis in dogs in Mosul city Iraqi Journal of Veterinary Sciences hepatzoon canis pcr dogs mosul |
| title | Molecular investigation of Hepatozoon canis in dogs in Mosul city |
| title_full | Molecular investigation of Hepatozoon canis in dogs in Mosul city |
| title_fullStr | Molecular investigation of Hepatozoon canis in dogs in Mosul city |
| title_full_unstemmed | Molecular investigation of Hepatozoon canis in dogs in Mosul city |
| title_short | Molecular investigation of Hepatozoon canis in dogs in Mosul city |
| title_sort | molecular investigation of hepatozoon canis in dogs in mosul city |
| topic | hepatzoon canis pcr dogs mosul |
| url | https://www.vetmedmosul.com/article_186155_730dc4a7888bd4b86224a31e76a20396.pdf |
| work_keys_str_mv | AT zeenadzaki molecularinvestigationofhepatozooncanisindogsinmosulcity AT wasanaalobaidii molecularinvestigationofhepatozooncanisindogsinmosulcity |