Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle
This study aimed to provide the first molecular characterization of bovine papillomavirus type 1 (BPV-1) in Iraq. BPV is a widely spread oncogenic virus in Iraqi cattle and is associated with the formation of both benign and malignant lesions, resulting in notable economic losses in dairy and beef c...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2016/2143024 |
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| author | Mohammed A. Hamad Ahmed M. Al-Shammari Shoni M. Odisho Nahi Y. Yaseen |
| author_facet | Mohammed A. Hamad Ahmed M. Al-Shammari Shoni M. Odisho Nahi Y. Yaseen |
| author_sort | Mohammed A. Hamad |
| collection | DOAJ |
| description | This study aimed to provide the first molecular characterization of bovine papillomavirus type 1 (BPV-1) in Iraq. BPV is a widely spread oncogenic virus in Iraqi cattle and is associated with the formation of both benign and malignant lesions, resulting in notable economic losses in dairy and beef cattle. In the current study, 140 cutaneous papilloma specimens were collected from cattle in central Iraq. These samples were submitted to histopathological examination, PCR, and sequencing analysis. The histopathology revealed that the main lesion type among the specimens was fibropapilloma. BPV-1 DNA was detected in 121 of the samples (86.42%) in Iraqi cattle as the main causative agent for the disease. A partial sequence for the E2, L2 genes, and complete sequence for the E5 gene were deposited in GenBank. Phylogenetic analysis confirmed the presence of BPV-1 and showed that the origin of infection may be imported European cattle. Obtaining a complete E5 gene sequence enabled us to perform structural predictions. This study presents the first report of BPV-1 infection in the Iraqi cattle and contributes to extending the knowledge of the origin of the spread of this disease. The results of this study will aid in the development of appropriate control measures and therapeutic strategies. |
| format | Article |
| id | doaj-art-e1963d89507b44b4986aa3830cbc189c |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-e1963d89507b44b4986aa3830cbc189c2025-02-03T06:46:57ZengWileyAdvances in Virology1687-86391687-86472016-01-01201610.1155/2016/21430242143024Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi CattleMohammed A. Hamad0Ahmed M. Al-Shammari1Shoni M. Odisho2Nahi Y. Yaseen3College of Veterinary Medicine, Al-Fallujah University, Al-Anbar 31002, IraqIraqi Center for Cancer and Medical Genetic Research, Al-Mustansiriya University, Al-Qadisiyah, Baghdad 1001, IraqCollege of Veterinary Medicine, Baghdad University, Baghdad 1001, IraqIraqi Center for Cancer and Medical Genetic Research, Al-Mustansiriya University, Al-Qadisiyah, Baghdad 1001, IraqThis study aimed to provide the first molecular characterization of bovine papillomavirus type 1 (BPV-1) in Iraq. BPV is a widely spread oncogenic virus in Iraqi cattle and is associated with the formation of both benign and malignant lesions, resulting in notable economic losses in dairy and beef cattle. In the current study, 140 cutaneous papilloma specimens were collected from cattle in central Iraq. These samples were submitted to histopathological examination, PCR, and sequencing analysis. The histopathology revealed that the main lesion type among the specimens was fibropapilloma. BPV-1 DNA was detected in 121 of the samples (86.42%) in Iraqi cattle as the main causative agent for the disease. A partial sequence for the E2, L2 genes, and complete sequence for the E5 gene were deposited in GenBank. Phylogenetic analysis confirmed the presence of BPV-1 and showed that the origin of infection may be imported European cattle. Obtaining a complete E5 gene sequence enabled us to perform structural predictions. This study presents the first report of BPV-1 infection in the Iraqi cattle and contributes to extending the knowledge of the origin of the spread of this disease. The results of this study will aid in the development of appropriate control measures and therapeutic strategies.http://dx.doi.org/10.1155/2016/2143024 |
| spellingShingle | Mohammed A. Hamad Ahmed M. Al-Shammari Shoni M. Odisho Nahi Y. Yaseen Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle Advances in Virology |
| title | Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle |
| title_full | Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle |
| title_fullStr | Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle |
| title_full_unstemmed | Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle |
| title_short | Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle |
| title_sort | molecular and phylogenetic analysis of bovine papillomavirus type 1 first report in iraqi cattle |
| url | http://dx.doi.org/10.1155/2016/2143024 |
| work_keys_str_mv | AT mohammedahamad molecularandphylogeneticanalysisofbovinepapillomavirustype1firstreportiniraqicattle AT ahmedmalshammari molecularandphylogeneticanalysisofbovinepapillomavirustype1firstreportiniraqicattle AT shonimodisho molecularandphylogeneticanalysisofbovinepapillomavirustype1firstreportiniraqicattle AT nahiyyaseen molecularandphylogeneticanalysisofbovinepapillomavirustype1firstreportiniraqicattle |