Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis
Abstract Studies have shown that the prognosis of dental implant treatment in patients with diabetes is not as good as that in the non-diabetes population. The nerve plays a crucial role in bone metabolism, but the role and the mechanism of peripheral nerves in regulating peri-implant osteogenesis u...
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BMC
2025-01-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03160-0 |
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| author | Yingying Wang Fang Qu Yaqin Wu Kengliang Lan Yingyi Shen Ziang Wu Qi Zhong Ximeng Cao Zhen Fan Chun Xu |
| author_facet | Yingying Wang Fang Qu Yaqin Wu Kengliang Lan Yingyi Shen Ziang Wu Qi Zhong Ximeng Cao Zhen Fan Chun Xu |
| author_sort | Yingying Wang |
| collection | DOAJ |
| description | Abstract Studies have shown that the prognosis of dental implant treatment in patients with diabetes is not as good as that in the non-diabetes population. The nerve plays a crucial role in bone metabolism, but the role and the mechanism of peripheral nerves in regulating peri-implant osteogenesis under Type 2 diabetes mellitus (T2DM) situation remains unclear. In this study, it was shown that high glucose-stimulated Schwann cells (SCs) inhibited peri-implant osteogenesis via their exosomes. SCs-derived exosomes were analyzed for their miRNA cargo, identifying miR-15b-5p as significantly downregulated in high glucose conditions. T2DM rats and patients exhibited decreased miR-15b-5p expression, correlating with impaired bone microarchitecture. Luciferase assays and Western blotting confirmed TXNIP as a direct miR-15b-5p target, implicating its involvement in ROS signaling and inflammation-related osteogenesis suppression. Furthermore, normal SCs exosomes improved bone parameters around dental implants in T2DM rats. These findings underscore the therapeutic potential of miR-15b-5p and normal SCs exosomes in mitigating poor peri-implant bone regeneration of T2DM patients, offering insights into the molecular mechanisms of peripheral nerves governing bone regeneration in diabetic conditions. Graphical Abstract |
| format | Article |
| id | doaj-art-e17bb43e063347b792413a0af7795339 |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | Journal of Nanobiotechnology |
| spelling | doaj-art-e17bb43e063347b792413a0af77953392025-02-02T12:41:07ZengBMCJournal of Nanobiotechnology1477-31552025-01-0123111910.1186/s12951-025-03160-0Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axisYingying Wang0Fang Qu1Yaqin Wu2Kengliang Lan3Yingyi Shen4Ziang Wu5Qi Zhong6Ximeng Cao7Zhen Fan8Chun Xu9Department of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineCollege of Stomatology, Shanghai Jiao Tong UniversityDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Implantology, School & Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji UniversityDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineAbstract Studies have shown that the prognosis of dental implant treatment in patients with diabetes is not as good as that in the non-diabetes population. The nerve plays a crucial role in bone metabolism, but the role and the mechanism of peripheral nerves in regulating peri-implant osteogenesis under Type 2 diabetes mellitus (T2DM) situation remains unclear. In this study, it was shown that high glucose-stimulated Schwann cells (SCs) inhibited peri-implant osteogenesis via their exosomes. SCs-derived exosomes were analyzed for their miRNA cargo, identifying miR-15b-5p as significantly downregulated in high glucose conditions. T2DM rats and patients exhibited decreased miR-15b-5p expression, correlating with impaired bone microarchitecture. Luciferase assays and Western blotting confirmed TXNIP as a direct miR-15b-5p target, implicating its involvement in ROS signaling and inflammation-related osteogenesis suppression. Furthermore, normal SCs exosomes improved bone parameters around dental implants in T2DM rats. These findings underscore the therapeutic potential of miR-15b-5p and normal SCs exosomes in mitigating poor peri-implant bone regeneration of T2DM patients, offering insights into the molecular mechanisms of peripheral nerves governing bone regeneration in diabetic conditions. Graphical Abstracthttps://doi.org/10.1186/s12951-025-03160-0Dental implantOsteogenesisType 2 diabetesSchwann cellsExosome |
| spellingShingle | Yingying Wang Fang Qu Yaqin Wu Kengliang Lan Yingyi Shen Ziang Wu Qi Zhong Ximeng Cao Zhen Fan Chun Xu Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis Journal of Nanobiotechnology Dental implant Osteogenesis Type 2 diabetes Schwann cells Exosome |
| title | Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis |
| title_full | Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis |
| title_fullStr | Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis |
| title_full_unstemmed | Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis |
| title_short | Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis |
| title_sort | peripheral nerves modulate the peri implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via mir 15b 5p txnip signaling axis |
| topic | Dental implant Osteogenesis Type 2 diabetes Schwann cells Exosome |
| url | https://doi.org/10.1186/s12951-025-03160-0 |
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