Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.)
Turnip, a subspecies of <i>Brassica rapa</i>, is one of the important root vegetable crops in China. Its roots, as significant product organs, possess values for consumption, feed, and medicinal purposes. A previous study localized a gene associated with root swelling traits. In this stu...
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2025-01-01
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| author | Wenyue Huang Shifan Zhang Fei Li Hui Zhang Rifei Sun Guoliang Li Shujiang Zhang |
| author_facet | Wenyue Huang Shifan Zhang Fei Li Hui Zhang Rifei Sun Guoliang Li Shujiang Zhang |
| author_sort | Wenyue Huang |
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| description | Turnip, a subspecies of <i>Brassica rapa</i>, is one of the important root vegetable crops in China. Its roots, as significant product organs, possess values for consumption, feed, and medicinal purposes. A previous study localized a gene associated with root swelling traits. In this study, we cloned the turnip root swelling-related gene <i>BraTSD2</i> (<i>TUMOROUS SHOOT DEVELOPMENT 2</i>) and performed a preliminary functional analysis. The bioinformatics analysis of <i>BraTSD2</i> indicated that the protein is likely to possess a transmembrane feature. Additionally, predictions of its secondary and tertiary structures suggested that BraTSD2 shares similarities with the structure of a pectin methyltransferase. Temporal and spatial expression analysis of <i>BraTSD2</i> using qPCR techniques indicated that the <i>BraTSD2</i> gene had the highest expression levels in roots and hypocotyls during the swelling stage. Analysis of the promoter cis-acting elements of the <i>BraTSD2</i> gene revealed that the promoter is regulated by signals associated with light response, abscisic acid, stress, salicylic acid, and MYBHI. The promoter of the turnip <i>BraTSD2</i> gene was expressed throughout the whole plant in <i>Arabidopsis thaliana</i>. Phenotypic observation and analysis of <i>A. thaliana</i> overexpression <i>BraTSD2</i> transgenic lines showed that plants overexpressing <i>BraTSD2</i> had fewer primary and lateral roots compared to the wild type, with lighter leaf color, and delayed development and flowering. A previously reported protein CGR3, associated with pectin methyltransferase function, interacts with protein BraTSD2. When the <i>BraTSD2</i> gene is overexpressed in <i>A. thaliana</i>, it can affect root morphology, which may be the basic structural condition for the generation of turnip root swelling traits. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Horticulturae |
| spelling | doaj-art-e15535d7ade84a3195b89a98943ba5df2025-01-24T13:34:32ZengMDPI AGHorticulturae2311-75242025-01-011113310.3390/horticulturae11010033Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.)Wenyue Huang0Shifan Zhang1Fei Li2Hui Zhang3Rifei Sun4Guoliang Li5Shujiang Zhang6State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaTurnip, a subspecies of <i>Brassica rapa</i>, is one of the important root vegetable crops in China. Its roots, as significant product organs, possess values for consumption, feed, and medicinal purposes. A previous study localized a gene associated with root swelling traits. In this study, we cloned the turnip root swelling-related gene <i>BraTSD2</i> (<i>TUMOROUS SHOOT DEVELOPMENT 2</i>) and performed a preliminary functional analysis. The bioinformatics analysis of <i>BraTSD2</i> indicated that the protein is likely to possess a transmembrane feature. Additionally, predictions of its secondary and tertiary structures suggested that BraTSD2 shares similarities with the structure of a pectin methyltransferase. Temporal and spatial expression analysis of <i>BraTSD2</i> using qPCR techniques indicated that the <i>BraTSD2</i> gene had the highest expression levels in roots and hypocotyls during the swelling stage. Analysis of the promoter cis-acting elements of the <i>BraTSD2</i> gene revealed that the promoter is regulated by signals associated with light response, abscisic acid, stress, salicylic acid, and MYBHI. The promoter of the turnip <i>BraTSD2</i> gene was expressed throughout the whole plant in <i>Arabidopsis thaliana</i>. Phenotypic observation and analysis of <i>A. thaliana</i> overexpression <i>BraTSD2</i> transgenic lines showed that plants overexpressing <i>BraTSD2</i> had fewer primary and lateral roots compared to the wild type, with lighter leaf color, and delayed development and flowering. A previously reported protein CGR3, associated with pectin methyltransferase function, interacts with protein BraTSD2. When the <i>BraTSD2</i> gene is overexpressed in <i>A. thaliana</i>, it can affect root morphology, which may be the basic structural condition for the generation of turnip root swelling traits.https://www.mdpi.com/2311-7524/11/1/33turniproot swelling<i>BraTSD2</i>functional analysis |
| spellingShingle | Wenyue Huang Shifan Zhang Fei Li Hui Zhang Rifei Sun Guoliang Li Shujiang Zhang Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) Horticulturae turnip root swelling <i>BraTSD2</i> functional analysis |
| title | Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) |
| title_full | Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) |
| title_fullStr | Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) |
| title_full_unstemmed | Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) |
| title_short | Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) |
| title_sort | cloning and functional analysis of i bratsd2 i associated with root swelling in turnip i brassica rapa i l |
| topic | turnip root swelling <i>BraTSD2</i> functional analysis |
| url | https://www.mdpi.com/2311-7524/11/1/33 |
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