Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.)
Turnip, a subspecies of <i>Brassica rapa</i>, is one of the important root vegetable crops in China. Its roots, as significant product organs, possess values for consumption, feed, and medicinal purposes. A previous study localized a gene associated with root swelling traits. In this stu...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/1/33 |
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| Summary: | Turnip, a subspecies of <i>Brassica rapa</i>, is one of the important root vegetable crops in China. Its roots, as significant product organs, possess values for consumption, feed, and medicinal purposes. A previous study localized a gene associated with root swelling traits. In this study, we cloned the turnip root swelling-related gene <i>BraTSD2</i> (<i>TUMOROUS SHOOT DEVELOPMENT 2</i>) and performed a preliminary functional analysis. The bioinformatics analysis of <i>BraTSD2</i> indicated that the protein is likely to possess a transmembrane feature. Additionally, predictions of its secondary and tertiary structures suggested that BraTSD2 shares similarities with the structure of a pectin methyltransferase. Temporal and spatial expression analysis of <i>BraTSD2</i> using qPCR techniques indicated that the <i>BraTSD2</i> gene had the highest expression levels in roots and hypocotyls during the swelling stage. Analysis of the promoter cis-acting elements of the <i>BraTSD2</i> gene revealed that the promoter is regulated by signals associated with light response, abscisic acid, stress, salicylic acid, and MYBHI. The promoter of the turnip <i>BraTSD2</i> gene was expressed throughout the whole plant in <i>Arabidopsis thaliana</i>. Phenotypic observation and analysis of <i>A. thaliana</i> overexpression <i>BraTSD2</i> transgenic lines showed that plants overexpressing <i>BraTSD2</i> had fewer primary and lateral roots compared to the wild type, with lighter leaf color, and delayed development and flowering. A previously reported protein CGR3, associated with pectin methyltransferase function, interacts with protein BraTSD2. When the <i>BraTSD2</i> gene is overexpressed in <i>A. thaliana</i>, it can affect root morphology, which may be the basic structural condition for the generation of turnip root swelling traits. |
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| ISSN: | 2311-7524 |