An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum

An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum

Abstract PfAgo, a thermophilic Argonaute nuclease from Pyrococcus furiosus, is widely used in various fields due to its high DNA‐guided DNA cleavage activity. However, its high‐temperature‐dependent cleavage activity largely restricts its applications in moderate‐temperature scenarios. In this study...

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Bibliographic Details
Main Authors: Longyu Wang, Xiaochen Xie, Fuyong Huang, Qiang Wei, Tianxin Cai, Na Yu, Shi Chen, Fei Wang, Wanping Chen, Chin‐yu Chen, Chunhua Li, Lixin Ma
Format: Article
Language:English
Published: Wiley 2025-08-01
Series:Advanced Science
Subjects:
cold adaptation
nucleases
PfAgo
rational engineering
thermophilic archaea
Online Access:https://doi.org/10.1002/advs.202416631
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