Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter
The development of a novel strategy for the measurement of SARS-CoV-2 IgG antibodies is of vital significance for <i>COVID-19</i> diagnosis and effect of vaccination evaluation. In this investigation, an SiO<sub>2</sub>@Au@CDs nanoparticle (NP)-based lateral flow immunoassay...
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MDPI AG
2024-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/12/1568 |
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| author | Rui Wang Junping Xue Guo Wei Yimeng Zhang Chuanliang Wang Jinhua Li Xuhui Geng Abbas Ostovan Lingxin Chen Zhihua Song |
| author_facet | Rui Wang Junping Xue Guo Wei Yimeng Zhang Chuanliang Wang Jinhua Li Xuhui Geng Abbas Ostovan Lingxin Chen Zhihua Song |
| author_sort | Rui Wang |
| collection | DOAJ |
| description | The development of a novel strategy for the measurement of SARS-CoV-2 IgG antibodies is of vital significance for <i>COVID-19</i> diagnosis and effect of vaccination evaluation. In this investigation, an SiO<sub>2</sub>@Au@CDs nanoparticle (NP)-based lateral flow immunoassay (LFIA) strip was fabricated and coupled with a miniaturized fluorimeter. The morphology features and particle sizes of the SiO<sub>2</sub>@Au@CDs NPs were characterized carefully, and the results indicated that the materials possess monodisperse, uniform, and spherical structures. Finally, this system was employed for SARS-CoV-2 IgG antibody test. In this work, the strategy for the SARS-CoV-2 IgG antibody test possesses several merits, such as speed (less than 15 min), high sensitivity (1.2 × 10<sup>−7</sup> mg/mL), broad linearity range (7.4 × 10<sup>−7</sup>~7.4 × 10<sup>−4</sup> mg/mL), accurate results, high selectivity, good stability, and low cost. Additionally, future trends in LFAs using quantum dot-based diagnostics are envisioned. |
| format | Article |
| id | doaj-art-e10e2803db8b4a73bbd7dd64169c6d30 |
| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-e10e2803db8b4a73bbd7dd64169c6d302025-08-20T02:01:04ZengMDPI AGBiomolecules2218-273X2024-12-011412156810.3390/biom14121568Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized FluorimeterRui Wang0Junping Xue1Guo Wei2Yimeng Zhang3Chuanliang Wang4Jinhua Li5Xuhui Geng6Abbas Ostovan7Lingxin Chen8Zhihua Song9School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaSchool of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaSchool of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaSchool of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaDepartment of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Key Laboratory of Deep-Sea Composition Detection Technology of Liaoning Province, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaDepartment of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Key Laboratory of Deep-Sea Composition Detection Technology of Liaoning Province, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaSchool of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaThe development of a novel strategy for the measurement of SARS-CoV-2 IgG antibodies is of vital significance for <i>COVID-19</i> diagnosis and effect of vaccination evaluation. In this investigation, an SiO<sub>2</sub>@Au@CDs nanoparticle (NP)-based lateral flow immunoassay (LFIA) strip was fabricated and coupled with a miniaturized fluorimeter. The morphology features and particle sizes of the SiO<sub>2</sub>@Au@CDs NPs were characterized carefully, and the results indicated that the materials possess monodisperse, uniform, and spherical structures. Finally, this system was employed for SARS-CoV-2 IgG antibody test. In this work, the strategy for the SARS-CoV-2 IgG antibody test possesses several merits, such as speed (less than 15 min), high sensitivity (1.2 × 10<sup>−7</sup> mg/mL), broad linearity range (7.4 × 10<sup>−7</sup>~7.4 × 10<sup>−4</sup> mg/mL), accurate results, high selectivity, good stability, and low cost. Additionally, future trends in LFAs using quantum dot-based diagnostics are envisioned.https://www.mdpi.com/2218-273X/14/12/1568SiO<sub>2</sub>@Au@CDs nanoparticleslateral flow immunoassaySARS-CoV-2 IgG antibodyfluorescence measurementhigh sensitivity and rapidity |
| spellingShingle | Rui Wang Junping Xue Guo Wei Yimeng Zhang Chuanliang Wang Jinhua Li Xuhui Geng Abbas Ostovan Lingxin Chen Zhihua Song Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter Biomolecules SiO<sub>2</sub>@Au@CDs nanoparticles lateral flow immunoassay SARS-CoV-2 IgG antibody fluorescence measurement high sensitivity and rapidity |
| title | Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter |
| title_full | Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter |
| title_fullStr | Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter |
| title_full_unstemmed | Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter |
| title_short | Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter |
| title_sort | fast and sensitive detection of anti sars cov 2 igg using sio sub 2 sub au cds nanoparticle based lateral flow immunoassay strip coupled with miniaturized fluorimeter |
| topic | SiO<sub>2</sub>@Au@CDs nanoparticles lateral flow immunoassay SARS-CoV-2 IgG antibody fluorescence measurement high sensitivity and rapidity |
| url | https://www.mdpi.com/2218-273X/14/12/1568 |
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