Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry
A novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical formulations by kinetic spectrophotometry has been developed and validated. It is based on the redox reaction where the thiol compound (RSH) redu...
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| Format: | Article |
| Language: | English |
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Sciendo
2021-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2021-0038 |
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| author | Kukoc-Modun Lea Biocic Maja Radić Njegomir |
| author_facet | Kukoc-Modun Lea Biocic Maja Radić Njegomir |
| author_sort | Kukoc-Modun Lea |
| collection | DOAJ |
| description | A novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical formulations by kinetic spectrophotometry has been developed and validated. It is based on the redox reaction where the thiol compound (RSH) reduces CuII-neocuproine complex to CuI-neocuproine complex. The non-steady state signal of the formed CuI- neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were validated. The calibration graph was linear in the concentration range from 8.0 × 10‒7 to 8.0 × 10‒5 mol L−1 for the initial rate method and from 6.0 × 10‒7 to 6.0 × 10−5 mol L−1 for the fixed time method, with the detection limits of 2.4 × 10−7 and 1.4 × 10‒7 mol L−1, resp. Levels of PEN, MPG and GSH in pharmaceutical formulations were successfully assayed by both methods. The advantages of the presented methods include sensitivity, short analysis time, ease of application and low cost. |
| format | Article |
| id | doaj-art-e10c701cf0154bfe842ba5e96b3726e8 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-e10c701cf0154bfe842ba5e96b3726e82025-02-02T00:31:54ZengSciendoActa Pharmaceutica1846-95582021-12-0171461963010.2478/acph-2021-0038Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometryKukoc-Modun Lea0Biocic Maja1Radić Njegomir2Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, 21000Split, CroatiaDepartment of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, 21000Split, CroatiaDepartment of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, 21000Split, CroatiaA novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical formulations by kinetic spectrophotometry has been developed and validated. It is based on the redox reaction where the thiol compound (RSH) reduces CuII-neocuproine complex to CuI-neocuproine complex. The non-steady state signal of the formed CuI- neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were validated. The calibration graph was linear in the concentration range from 8.0 × 10‒7 to 8.0 × 10‒5 mol L−1 for the initial rate method and from 6.0 × 10‒7 to 6.0 × 10−5 mol L−1 for the fixed time method, with the detection limits of 2.4 × 10−7 and 1.4 × 10‒7 mol L−1, resp. Levels of PEN, MPG and GSH in pharmaceutical formulations were successfully assayed by both methods. The advantages of the presented methods include sensitivity, short analysis time, ease of application and low cost.https://doi.org/10.2478/acph-2021-0038penicillaminetioproninglutathionekinetic spectrophotometrypharmaceutical formulations |
| spellingShingle | Kukoc-Modun Lea Biocic Maja Radić Njegomir Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry Acta Pharmaceutica penicillamine tiopronin glutathione kinetic spectrophotometry pharmaceutical formulations |
| title | Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry |
| title_full | Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry |
| title_fullStr | Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry |
| title_full_unstemmed | Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry |
| title_short | Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry |
| title_sort | determination of penicillamine tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry |
| topic | penicillamine tiopronin glutathione kinetic spectrophotometry pharmaceutical formulations |
| url | https://doi.org/10.2478/acph-2021-0038 |
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