Emulation of an ELISA Reader and Quantitative Image Analysis Using the Computer Program Digital-OD
Here, we present a Microsoft® Windows® application that has been written to emulate a microplate reader. After scanning a standard 96-well microplate, color gradations in the slots can be analyzed quantitatively and be used for the estimation of enzyme kinetics, binding assays or concentration deter...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98246bc02 |
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| Summary: | Here, we present a Microsoft® Windows® application that has been written to emulate a microplate reader. After scanning a standard 96-well microplate, color gradations in the slots can be analyzed quantitatively and be used for the estimation of enzyme kinetics, binding assays or concentration determination. In a test, series accuracy of the program was determined. We show that the returned optical densities correlated highly with the true concentrations (r > 0.97). Thus, the software is useful for a wide number of applications. Further, the program is easy to handle and is available free of charge. |
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| ISSN: | 0736-6205 1940-9818 |