CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma
Nasopharyngeal carcinoma (NPC) is a kind of malignant tumor with high metastasis. Circular RNAs (circRNAs) are involved in tumor progression, but their functions and mechanisms are not well understood. Vasculogenic mimicry (VM) has been discovered as an alternative way to supply tumor nutrition and...
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | Molecular Oncology |
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| Online Access: | https://doi.org/10.1002/1878-0261.13821 |
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| author | Chunmei Fan Fenghua Tan Jie Wu Zhaoyang Zeng Wenjia Guo He Huang Wei Xiong |
| author_facet | Chunmei Fan Fenghua Tan Jie Wu Zhaoyang Zeng Wenjia Guo He Huang Wei Xiong |
| author_sort | Chunmei Fan |
| collection | DOAJ |
| description | Nasopharyngeal carcinoma (NPC) is a kind of malignant tumor with high metastasis. Circular RNAs (circRNAs) are involved in tumor progression, but their functions and mechanisms are not well understood. Vasculogenic mimicry (VM) has been discovered as an alternative way to supply tumor nutrition and accelerate tumor progression, including NPC. We previously found that circCCNB1 (derived from cyclin B1) could inhibit the migration and invasion of NPC cells by binding to nuclear factor 90 (NF90), however, whether circCCNB1 has additional biological functions is still unclear. In this study, the effects of circCCNB1 binding to NF90 on the generation of miR‐15b‐5p and miR‐7‐1‐3p were detected using qRT‐PCR, western blotting, RNA pulldown, ribonucleoprotein immunoprecipitation and truncated experiments. VM formation assays were used to assess their biological functions. We found that circCCNB1 promoted the processing and generation of miR‐15b‐5p and miR‐7‐1‐3p through competitive binding to NF90, thereby inhibiting the expression of calumenin (CALU), kinesin family member 1B (KIF1B), RNA polymerase III subunit G (POLR3G), ultimately decreasing the VM of NPC cells. This study not only reveals a new function of circCCNB1 in NPC, but also provides new insights for targeting angiogenesis therapy. |
| format | Article |
| id | doaj-art-e0f5d33575b64cb99cd6c3ba8d4b5b68 |
| institution | OA Journals |
| issn | 1574-7891 1878-0261 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Oncology |
| spelling | doaj-art-e0f5d33575b64cb99cd6c3ba8d4b5b682025-08-20T02:32:38ZengWileyMolecular Oncology1574-78911878-02612025-06-011961876189310.1002/1878-0261.13821CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinomaChunmei Fan0Fenghua Tan1Jie Wu2Zhaoyang Zeng3Wenjia Guo4He Huang5Wei Xiong6NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine Central South University Changsha ChinaKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute Central South University Changsha ChinaKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute Central South University Changsha ChinaNHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine Central South University Changsha ChinaDepartment of Cancer Research Institute Affiliated Cancer Hospital of Xinjiang Medical University Urumqi ChinaDepartment of Histology and Embryology, Xiangya School of Medicine Central South University Changsha ChinaNHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine Central South University Changsha ChinaNasopharyngeal carcinoma (NPC) is a kind of malignant tumor with high metastasis. Circular RNAs (circRNAs) are involved in tumor progression, but their functions and mechanisms are not well understood. Vasculogenic mimicry (VM) has been discovered as an alternative way to supply tumor nutrition and accelerate tumor progression, including NPC. We previously found that circCCNB1 (derived from cyclin B1) could inhibit the migration and invasion of NPC cells by binding to nuclear factor 90 (NF90), however, whether circCCNB1 has additional biological functions is still unclear. In this study, the effects of circCCNB1 binding to NF90 on the generation of miR‐15b‐5p and miR‐7‐1‐3p were detected using qRT‐PCR, western blotting, RNA pulldown, ribonucleoprotein immunoprecipitation and truncated experiments. VM formation assays were used to assess their biological functions. We found that circCCNB1 promoted the processing and generation of miR‐15b‐5p and miR‐7‐1‐3p through competitive binding to NF90, thereby inhibiting the expression of calumenin (CALU), kinesin family member 1B (KIF1B), RNA polymerase III subunit G (POLR3G), ultimately decreasing the VM of NPC cells. This study not only reveals a new function of circCCNB1 in NPC, but also provides new insights for targeting angiogenesis therapy.https://doi.org/10.1002/1878-0261.13821circCCNB1miRNA processingnasopharyngeal carcinomaNF90vasculogenic mimicry |
| spellingShingle | Chunmei Fan Fenghua Tan Jie Wu Zhaoyang Zeng Wenjia Guo He Huang Wei Xiong CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma Molecular Oncology circCCNB1 miRNA processing nasopharyngeal carcinoma NF90 vasculogenic mimicry |
| title | CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma |
| title_full | CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma |
| title_fullStr | CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma |
| title_full_unstemmed | CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma |
| title_short | CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma |
| title_sort | circccnb1 inhibits vasculogenic mimicry by sequestering nf90 to promote mir 15b 5p and mir 7 1 3p processing in nasopharyngeal carcinoma |
| topic | circCCNB1 miRNA processing nasopharyngeal carcinoma NF90 vasculogenic mimicry |
| url | https://doi.org/10.1002/1878-0261.13821 |
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