Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean
The present study was initiated to determine whether isolates from soil and roots of soybean plants can express nitrogenase activity when grown in the absence of plant host. The study was conducted to answer the question “can benefit gained by” the interaction between Actinomycetes and Rhizobium sym...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | International Journal of Agronomy |
| Online Access: | http://dx.doi.org/10.1155/2018/4371623 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546167215357952 |
|---|---|
| author | Asmiaty Sahur Ambo Ala Baharuddin Patandjengi Elkawakib Syam’un |
| author_facet | Asmiaty Sahur Ambo Ala Baharuddin Patandjengi Elkawakib Syam’un |
| author_sort | Asmiaty Sahur |
| collection | DOAJ |
| description | The present study was initiated to determine whether isolates from soil and roots of soybean plants can express nitrogenase activity when grown in the absence of plant host. The study was conducted to answer the question “can benefit gained by” the interaction between Actinomycetes and Rhizobium symbiosis with legume. Thirty-five isolates identified as Rhizobium and twenty-one Actinomycetes were isolated from the rhizosphere of soybean plants and identified by morphological character, biochemical content identified. Fifty-six isolates were tested for their capabilities of N2 fixation and siderophore production. The isolated rhizobacteria were grown in N-free media, and twelve of them showed a good growth on the Burk’s N-free media. Almost all strains produced siderophores; however, the production level was very low, and only the strain AK 10 released considerable amounts of this metabolite. One strain of Actinomycetes was selected to test their interactions with Rhizobium. Coinoculation of Actinomycetes and Rhizobium produced synergic benefits on plant growth and get protection from the production of siderophore. |
| format | Article |
| id | doaj-art-e0f0e47992e34a77855b7eb681198a03 |
| institution | Kabale University |
| issn | 1687-8159 1687-8167 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Agronomy |
| spelling | doaj-art-e0f0e47992e34a77855b7eb681198a032025-02-03T07:23:48ZengWileyInternational Journal of Agronomy1687-81591687-81672018-01-01201810.1155/2018/43716234371623Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of SoybeanAsmiaty Sahur0Ambo Ala1Baharuddin Patandjengi2Elkawakib Syam’un3Department of Agronomy, Faculty of Agriculture, Hasanuddin University, Makassar, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Hasanuddin University, Makassar, IndonesiaResearch Center (PKP), Hasanuddin University, Makassar, South Sulawesi 90245, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Hasanuddin University, Makassar, IndonesiaThe present study was initiated to determine whether isolates from soil and roots of soybean plants can express nitrogenase activity when grown in the absence of plant host. The study was conducted to answer the question “can benefit gained by” the interaction between Actinomycetes and Rhizobium symbiosis with legume. Thirty-five isolates identified as Rhizobium and twenty-one Actinomycetes were isolated from the rhizosphere of soybean plants and identified by morphological character, biochemical content identified. Fifty-six isolates were tested for their capabilities of N2 fixation and siderophore production. The isolated rhizobacteria were grown in N-free media, and twelve of them showed a good growth on the Burk’s N-free media. Almost all strains produced siderophores; however, the production level was very low, and only the strain AK 10 released considerable amounts of this metabolite. One strain of Actinomycetes was selected to test their interactions with Rhizobium. Coinoculation of Actinomycetes and Rhizobium produced synergic benefits on plant growth and get protection from the production of siderophore.http://dx.doi.org/10.1155/2018/4371623 |
| spellingShingle | Asmiaty Sahur Ambo Ala Baharuddin Patandjengi Elkawakib Syam’un Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean International Journal of Agronomy |
| title | Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean |
| title_full | Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean |
| title_fullStr | Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean |
| title_full_unstemmed | Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean |
| title_short | Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean |
| title_sort | effect of seed inoculation with actinomycetes and rhizobium isolated from indigenous soybean and rhizosphere on nitrogen fixation growth and yield of soybean |
| url | http://dx.doi.org/10.1155/2018/4371623 |
| work_keys_str_mv | AT asmiatysahur effectofseedinoculationwithactinomycetesandrhizobiumisolatedfromindigenoussoybeanandrhizosphereonnitrogenfixationgrowthandyieldofsoybean AT amboala effectofseedinoculationwithactinomycetesandrhizobiumisolatedfromindigenoussoybeanandrhizosphereonnitrogenfixationgrowthandyieldofsoybean AT baharuddinpatandjengi effectofseedinoculationwithactinomycetesandrhizobiumisolatedfromindigenoussoybeanandrhizosphereonnitrogenfixationgrowthandyieldofsoybean AT elkawakibsyamun effectofseedinoculationwithactinomycetesandrhizobiumisolatedfromindigenoussoybeanandrhizosphereonnitrogenfixationgrowthandyieldofsoybean |