Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV Infection
Introduction: Viral infections cause oxygen deprivation, leading to hypoxia or anoxia in certain tissues. The limitation of mitochondrial respiration is one of the major events during hypoxia that induces alternative metabolic activities and increased levels of certain biomolecules such as nitric ox...
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MDPI AG
2024-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/9/1172 |
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| author | Clara Matei Ilinca Nicolae Madalina Irina Mitran Cristina Iulia Mitran Corina Daniela Ene Gheorghe Nicolae Simona Roxana Georgescu Mircea Tampa |
| author_facet | Clara Matei Ilinca Nicolae Madalina Irina Mitran Cristina Iulia Mitran Corina Daniela Ene Gheorghe Nicolae Simona Roxana Georgescu Mircea Tampa |
| author_sort | Clara Matei |
| collection | DOAJ |
| description | Introduction: Viral infections cause oxygen deprivation, leading to hypoxia or anoxia in certain tissues. The limitation of mitochondrial respiration is one of the major events during hypoxia that induces alternative metabolic activities and increased levels of certain biomolecules such as nitric oxide (NO) metabolites. In this study, we aimed to investigate the role of NO metabolites and hypoxia in HPV infection. Materials and Methods: We included 36 patients with palmoplantar warts and 36 healthy subjects and performed serum determinations of NO metabolites (direct nitrite, total nitrite, nitrate, and 3-nitrotyrosine) and HIF1α, a marker of hypoxia. Results: We found elevated serum levels in NO metabolites and HIF1α, and decreased direct nitrite/nitrate ratios in patients with warts versus controls. Additionally, we identified statistically significant positive correlations between NO metabolites and HIF1α levels, except for 3-nitrotyrosine. Conclusions: Our findings show that HPV infection causes hypoxia and alterations in NO metabolism and suggest a link between wart development and cellular stress. Our research could provide new insights for a comprehensive understanding of the pathogenesis of cutaneous HPV infections. |
| format | Article |
| id | doaj-art-e0efa7aecf8a40b486842099d855fb61 |
| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-e0efa7aecf8a40b486842099d855fb612025-08-20T01:56:05ZengMDPI AGBiomolecules2218-273X2024-09-01149117210.3390/biom14091172Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV InfectionClara Matei0Ilinca Nicolae1Madalina Irina Mitran2Cristina Iulia Mitran3Corina Daniela Ene4Gheorghe Nicolae5Simona Roxana Georgescu6Mircea Tampa7Department of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, ‘Victor Babes’ Clinical Hospital for Infectious Diseases, 030303 Bucharest, RomaniaDepartment of Microbiology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Microbiology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartments of Nephrology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaFaculty of Psychology, Babeș-Bolyai University, 400347 Cluj-Napoca, RomaniaDepartment of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaIntroduction: Viral infections cause oxygen deprivation, leading to hypoxia or anoxia in certain tissues. The limitation of mitochondrial respiration is one of the major events during hypoxia that induces alternative metabolic activities and increased levels of certain biomolecules such as nitric oxide (NO) metabolites. In this study, we aimed to investigate the role of NO metabolites and hypoxia in HPV infection. Materials and Methods: We included 36 patients with palmoplantar warts and 36 healthy subjects and performed serum determinations of NO metabolites (direct nitrite, total nitrite, nitrate, and 3-nitrotyrosine) and HIF1α, a marker of hypoxia. Results: We found elevated serum levels in NO metabolites and HIF1α, and decreased direct nitrite/nitrate ratios in patients with warts versus controls. Additionally, we identified statistically significant positive correlations between NO metabolites and HIF1α levels, except for 3-nitrotyrosine. Conclusions: Our findings show that HPV infection causes hypoxia and alterations in NO metabolism and suggest a link between wart development and cellular stress. Our research could provide new insights for a comprehensive understanding of the pathogenesis of cutaneous HPV infections.https://www.mdpi.com/2218-273X/14/9/1172HPVwartsnitric oxide metaboliteshypoxiaHIF1α |
| spellingShingle | Clara Matei Ilinca Nicolae Madalina Irina Mitran Cristina Iulia Mitran Corina Daniela Ene Gheorghe Nicolae Simona Roxana Georgescu Mircea Tampa Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV Infection Biomolecules HPV warts nitric oxide metabolites hypoxia HIF1α |
| title | Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV Infection |
| title_full | Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV Infection |
| title_fullStr | Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV Infection |
| title_full_unstemmed | Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV Infection |
| title_short | Biomolecular Dynamics of Nitric Oxide Metabolites and HIF1α in HPV Infection |
| title_sort | biomolecular dynamics of nitric oxide metabolites and hif1α in hpv infection |
| topic | HPV warts nitric oxide metabolites hypoxia HIF1α |
| url | https://www.mdpi.com/2218-273X/14/9/1172 |
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