Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation
Background The incidence of lumbar disk herniation (LDH) is usually caused by lumbar disk degeneration. Surgery is a common treatment strategy for LDH, but it can recur, resulting in recurrent disk herniation (RDH).Purpose To explore the predictive value of hsa-miR-4741 and LILRB2 in the prognosis o...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/08941939.2025.2458180 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832566648172707840 |
|---|---|
| author | Zhendong He Nan Zheng Xiu-Quan Guo Gang-Gang Wang Mingjian Lin |
| author_facet | Zhendong He Nan Zheng Xiu-Quan Guo Gang-Gang Wang Mingjian Lin |
| author_sort | Zhendong He |
| collection | DOAJ |
| description | Background The incidence of lumbar disk herniation (LDH) is usually caused by lumbar disk degeneration. Surgery is a common treatment strategy for LDH, but it can recur, resulting in recurrent disk herniation (RDH).Purpose To explore the predictive value of hsa-miR-4741 and LILRB2 in the prognosis of LDH surgery and the mechanism of nucleus pulposus senescence.Method The ROC curves of RDH based on hsa-miR-4741 and LILRB2 were constructed to evaluate their predictive values in the prognosis of LDH surgery. Human nucleus pulposus cells (NPC) was treated by TNF-α to construct a cell senescence model, studying the senescence mechanism. Oxidative stress and senescence markers were detected after overexpression of hsa-miR-4741 and LILRB2 to evaluate their effects on the senescence of NPC. Dual luciferase assay and the transfection of hsa-miR-4741 mimics or inhibitor were used to investigate the targeted regulation of it to LILRB2.Results The combination of hsa-miR-4741 and LILRB2 showed higher accuracy in predicting the outcome of RDH (AUC = 0.9367), compared with a single molecule. Overexpression of hsa-miR-4741 enhanced TNF-α-induced oxidative stress and senescence, while LILRB2 overexpression had the opposite effect. Hsa-miR-4741 mimics attenuated the luciferase activity of NPC transfected with wt-LILRB2 vector and significantly down-regulated LILRB2 expression. In addition, the antioxidant NAC reversed the promotion of hsa-miR-4741 on NPC senescence.Conclusion The combination of hsa-miR-4741 and LILRB2 was a good predictor of LDH prognosis. Hsa-miR-4741 promoted oxidative stress-induced NPC senescence by negatively regulating LILRB2. |
| format | Article |
| id | doaj-art-e0e4d6979a0a4fa0a652c1487cae7136 |
| institution | Kabale University |
| issn | 0894-1939 1521-0553 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj-art-e0e4d6979a0a4fa0a652c1487cae71362025-02-03T01:03:43ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532025-12-0138110.1080/08941939.2025.2458180Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc HerniationZhendong He0Nan Zheng1Xiu-Quan Guo2Gang-Gang Wang3Mingjian Lin4Department of Spinal Surgery, The Third People’s Hospital of Gansu Province, Lanzhou, Gansu, ChinaDepartment of Orthopedics, The 983 Hospital of Joint Logistic Support Force of PLA, Tianjin, ChinaDepartment of Spinal Surgery, Zhucheng People’s Hospital, Weifang, Shandong, ChinaDepartment of Hand and Foot Surgery, Zhucheng People’s Hospital, Weifang, Shandong, ChinaDepartment of Neurosurgery, Gaozhou People’s Hospital, Maoming, Guangdong, ChinaBackground The incidence of lumbar disk herniation (LDH) is usually caused by lumbar disk degeneration. Surgery is a common treatment strategy for LDH, but it can recur, resulting in recurrent disk herniation (RDH).Purpose To explore the predictive value of hsa-miR-4741 and LILRB2 in the prognosis of LDH surgery and the mechanism of nucleus pulposus senescence.Method The ROC curves of RDH based on hsa-miR-4741 and LILRB2 were constructed to evaluate their predictive values in the prognosis of LDH surgery. Human nucleus pulposus cells (NPC) was treated by TNF-α to construct a cell senescence model, studying the senescence mechanism. Oxidative stress and senescence markers were detected after overexpression of hsa-miR-4741 and LILRB2 to evaluate their effects on the senescence of NPC. Dual luciferase assay and the transfection of hsa-miR-4741 mimics or inhibitor were used to investigate the targeted regulation of it to LILRB2.Results The combination of hsa-miR-4741 and LILRB2 showed higher accuracy in predicting the outcome of RDH (AUC = 0.9367), compared with a single molecule. Overexpression of hsa-miR-4741 enhanced TNF-α-induced oxidative stress and senescence, while LILRB2 overexpression had the opposite effect. Hsa-miR-4741 mimics attenuated the luciferase activity of NPC transfected with wt-LILRB2 vector and significantly down-regulated LILRB2 expression. In addition, the antioxidant NAC reversed the promotion of hsa-miR-4741 on NPC senescence.Conclusion The combination of hsa-miR-4741 and LILRB2 was a good predictor of LDH prognosis. Hsa-miR-4741 promoted oxidative stress-induced NPC senescence by negatively regulating LILRB2.https://www.tandfonline.com/doi/10.1080/08941939.2025.2458180Lumbar disk herniationhsa-miR-4741LILRB2nucleus pulposus cellssenescence |
| spellingShingle | Zhendong He Nan Zheng Xiu-Quan Guo Gang-Gang Wang Mingjian Lin Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation Journal of Investigative Surgery Lumbar disk herniation hsa-miR-4741 LILRB2 nucleus pulposus cells senescence |
| title | Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation |
| title_full | Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation |
| title_fullStr | Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation |
| title_full_unstemmed | Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation |
| title_short | Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation |
| title_sort | effects of hsa mir 4741 lilrb2 on senescence of nucleus pulposus cells and their prognostic values in lumbar disc herniation |
| topic | Lumbar disk herniation hsa-miR-4741 LILRB2 nucleus pulposus cells senescence |
| url | https://www.tandfonline.com/doi/10.1080/08941939.2025.2458180 |
| work_keys_str_mv | AT zhendonghe effectsofhsamir4741lilrb2onsenescenceofnucleuspulposuscellsandtheirprognosticvaluesinlumbardischerniation AT nanzheng effectsofhsamir4741lilrb2onsenescenceofnucleuspulposuscellsandtheirprognosticvaluesinlumbardischerniation AT xiuquanguo effectsofhsamir4741lilrb2onsenescenceofnucleuspulposuscellsandtheirprognosticvaluesinlumbardischerniation AT ganggangwang effectsofhsamir4741lilrb2onsenescenceofnucleuspulposuscellsandtheirprognosticvaluesinlumbardischerniation AT mingjianlin effectsofhsamir4741lilrb2onsenescenceofnucleuspulposuscellsandtheirprognosticvaluesinlumbardischerniation |