Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway
Background. Clinically, electroacupuncture (EA) is the most common therapy for aging-related cognitive impairment (CI). However, the underlying pathomechanism remains unidentified. The aims of this study were to observe the effect of EA on cognitive function and explore the potential mechanism by wh...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/8853720 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568531898597376 |
|---|---|
| author | Zhitao Hou Ruijin Qiu Qingshuang Wei Yitian Liu Meng Wang Tingting Mei Yue Zhang Liying Song Xianming Shao Hongcai Shang Jing Chen Zhongren Sun |
| author_facet | Zhitao Hou Ruijin Qiu Qingshuang Wei Yitian Liu Meng Wang Tingting Mei Yue Zhang Liying Song Xianming Shao Hongcai Shang Jing Chen Zhongren Sun |
| author_sort | Zhitao Hou |
| collection | DOAJ |
| description | Background. Clinically, electroacupuncture (EA) is the most common therapy for aging-related cognitive impairment (CI). However, the underlying pathomechanism remains unidentified. The aims of this study were to observe the effect of EA on cognitive function and explore the potential mechanism by which EA acts on the NLRP3/caspase-1 signaling pathway. Main Methods. Thirty male SAMP8 mice were randomly divided into the model, the 2 Hz EA and 10 Hz EA groups. Ten male SAMR1 mice were assigned to the control group. Cognitive function was assessed through the Morris water maze test. Hippocampal morphology and cell death were observed by HE and TUNEL staining, respectively. The serum IL-1β, IL-6, IL-18, and TNF-α levels were measured by ELISA. Hippocampal NLRP3, ASC, caspase-1, GSDM-D, IL-1β, IL-18, Aβ, and tau proteins were detected by Western blotting. Key Findings. Cognitive function, hippocampal morphology, and TUNEL-positive cell counts were improved by both EA frequencies. The serum IL-1β, IL-6, IL-18, and TNF-α levels were decreased by EA treatment. However, 10 Hz EA reduced the number of TUNEL-positive cells in the CA1 region and serum IL-1β and IL-6 levels more effectively than 2 Hz EA. NLRP3/caspase-1 pathway-related proteins were significantly downregulated by EA, but 2 Hz EA did not effectively reduce ASC protein expression. Interestingly, both EA frequencies failed to reduce the expression of Aβ and tau proteins. Significance. The effects of 10 Hz EA at the GV20 and ST36 acupoints on the NLRP3/caspase-1 signaling pathway may be a mechanism by which this treatment relieves aging-related CI in mice. |
| format | Article |
| id | doaj-art-e0dccedc1ce44960861246af08a35f24 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-e0dccedc1ce44960861246af08a35f242025-02-03T00:58:52ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/88537208853720Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 PathwayZhitao Hou0Ruijin Qiu1Qingshuang Wei2Yitian Liu3Meng Wang4Tingting Mei5Yue Zhang6Liying Song7Xianming Shao8Hongcai Shang9Jing Chen10Zhongren Sun11College of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaKey Laboratory of Chinese Internal Medicine of the Ministry of Education, Dongzhimen Hospital Affiliated with Beijing University of Chinese Medicine, Beijing 100700, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, ChinaCollege of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaCollege of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaCollege of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaCollege of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaCollege of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaKey Laboratory of Chinese Internal Medicine of the Ministry of Education, Dongzhimen Hospital Affiliated with Beijing University of Chinese Medicine, Beijing 100700, ChinaCollege of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaCollege of Basic Medical and Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, ChinaBackground. Clinically, electroacupuncture (EA) is the most common therapy for aging-related cognitive impairment (CI). However, the underlying pathomechanism remains unidentified. The aims of this study were to observe the effect of EA on cognitive function and explore the potential mechanism by which EA acts on the NLRP3/caspase-1 signaling pathway. Main Methods. Thirty male SAMP8 mice were randomly divided into the model, the 2 Hz EA and 10 Hz EA groups. Ten male SAMR1 mice were assigned to the control group. Cognitive function was assessed through the Morris water maze test. Hippocampal morphology and cell death were observed by HE and TUNEL staining, respectively. The serum IL-1β, IL-6, IL-18, and TNF-α levels were measured by ELISA. Hippocampal NLRP3, ASC, caspase-1, GSDM-D, IL-1β, IL-18, Aβ, and tau proteins were detected by Western blotting. Key Findings. Cognitive function, hippocampal morphology, and TUNEL-positive cell counts were improved by both EA frequencies. The serum IL-1β, IL-6, IL-18, and TNF-α levels were decreased by EA treatment. However, 10 Hz EA reduced the number of TUNEL-positive cells in the CA1 region and serum IL-1β and IL-6 levels more effectively than 2 Hz EA. NLRP3/caspase-1 pathway-related proteins were significantly downregulated by EA, but 2 Hz EA did not effectively reduce ASC protein expression. Interestingly, both EA frequencies failed to reduce the expression of Aβ and tau proteins. Significance. The effects of 10 Hz EA at the GV20 and ST36 acupoints on the NLRP3/caspase-1 signaling pathway may be a mechanism by which this treatment relieves aging-related CI in mice.http://dx.doi.org/10.1155/2020/8853720 |
| spellingShingle | Zhitao Hou Ruijin Qiu Qingshuang Wei Yitian Liu Meng Wang Tingting Mei Yue Zhang Liying Song Xianming Shao Hongcai Shang Jing Chen Zhongren Sun Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway Neural Plasticity |
| title | Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway |
| title_full | Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway |
| title_fullStr | Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway |
| title_full_unstemmed | Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway |
| title_short | Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway |
| title_sort | electroacupuncture improves cognitive function in senescence accelerated p8 samp8 mice via the nlrp3 caspase 1 pathway |
| url | http://dx.doi.org/10.1155/2020/8853720 |
| work_keys_str_mv | AT zhitaohou electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT ruijinqiu electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT qingshuangwei electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT yitianliu electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT mengwang electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT tingtingmei electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT yuezhang electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT liyingsong electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT xianmingshao electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT hongcaishang electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT jingchen electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway AT zhongrensun electroacupunctureimprovescognitivefunctioninsenescenceacceleratedp8samp8miceviathenlrp3caspase1pathway |